Bioengineering Research Partnership in Total Joint Replacements

项目摘要 这项建议是为了响应项目公告(PAR-07-352)，标题为“生物工程研究 在这个产学研合作伙伴关系中，我们专注于开发和测试 用于最大限度地减少关节间磨损的金刚石表面和聚乙烯表面 矫形外科设备中的部件。磨损引起的并发症包括部件松动， 有害的生物反应，骨溶解，机械不稳定，关节活动度降低，疼痛增加， 最终植入失败。在这个项目中，UAB和Smith&Nephew，Inc.合作开发和测试 金属(CoCrMo或Ti-6Al-4V)矫形装置上独特的纳米结构多层金刚石涂层 降低铰接部件中摩擦和磨损的目标。我们建议进行一项为期五年的研究，重点是 以下具体目标：具体目标1：开发减少摩擦和磨损的纳米技术 用化学气相沉积法(CVD)制备髋关节和膝关节金属植入物的承重部件 一种具有纳米和微结构层交替的多层结构的金刚石涂层 组件。具体目标2：证明多层金刚石涂层(用于钻石上的涂层) 钻石或聚乙烯上的钻石铰接)与单一的- 层钻石对钻石铰接或金属对聚乙烯或金属对金属对目前 用于商业整形外科设备。具体目标3：进行与钻石有关的磨损模拟器研究 使用行业标准多轴髋关节和膝关节模拟器的涂层髋关节和膝关节关节组件 位于Smith&Nephew，Inc.。具体目标4：评估细胞对钻石磨屑颗粒的反应， 与聚乙烯和钴铬碎屑颗粒相比。具体目标5：进行活体研究 表征对照和钻石生物材料的初始短期有机和细胞响应 整体的生物相容性特征。从摩擦学研究中收集的磨屑将被植入(注射) 在成年大鼠的滑膜样气囊模型中短期(几小时到几天)，以评估临床， 细胞、组织学和细胞因子生物相容性概况。小梁骨区植入间盘 兔将评估最初的组织液和血液与对照和涂层植入物表面的相互作用时间 从几个小时到几个月不等。这项拟议的BRP的总体临床影响将是改善的。 将全部关节更换的使用寿命延长到30年以上，从而大大缩短了 需要反复进行多次外科手术。

Project Summary This proposal is in response to program announcement (PAR-07-352) entitled "Bioengineering Research Partnerships (BRP)". In this University-Industry Partnership, we are focused on development and testing of diamond-on-diamond and diamond-on-polyethylene surfaces for minimizing wear between articulation components in orthopaedic devices. Complications arising from wear include component loosening, deleterious biological responses, osteolysis, mechanical instability, decreased joint mobility, increased pain, and ultimately implant failure. In this project, UAB and Smith & Nephew, Inc. team up to develop and test unique nanostructured multilayer diamond coatings on metal (CoCrMo or Ti-6Al-4V) orthopaedic devices with the goal of reducing friction and wear in articulation components. We propose a five-year study to focus on the following specific aims: Specific Aim 1: Develop nanotechnology for reducing friction and wear on the articulating, load-bearing components of hip and knee metallic implants by chemical vapor deposition (CVD) of a diamond coating having a multilayer structure with alternating nano- and micro-structural layered components. Specific Aim 2: Demonstrate that the multilayer diamond coatings (intended for diamond-on- diamond or diamond-on-polyethylene articulation) exhibit improved wear resistance when compared to single- layer diamond-on-diamond articulation or to the metal-on-polyethylene or metal-on-metal couples currently used in commercial orthopaedic devices. Specific Aim 3: Perform wear simulator studies involving diamond coated hip and knee articulation components using the industry-standard multi-axis hip and knee simulators located at Smith & Nephew, Inc. Specific Aim 4: Evaluate cellular response to diamond wear debris particles, as compared to polyethylene and cobalt chrome debris particles. Specific Aim 5: Perform in vivo investigations to characterize the initial short term organic and cellular responses of control and diamond biomaterials to overall biocompatibility profiles. Wear debris collected from the tribological studies will be implanted (injected) into the synovial-like air pouch model in the adult rat for short term (hours to days) in order to assess clinical, cellular, histological and cytokine biocompatibility profiles. Implants of discs into trabecular bone regions of rabbits will assess initial tissue fluid and blood interactions with control and coated implant surfaces for time points ranging from hours to months. The overall clinical impact of this proposed BRP would be in improving the service life time of total joint replacements to more than thirty years and hence dramatically reducing the need for recurrent multiple surgical procedures.