Histology Core

项目总结（见说明）： 肌肉骨骼组织的结构和组成是定制的，以满足其苛刻的功能。 随着损伤，这些组织的结构和组成恶化，导致机械功能下降或丧失。肌肉骨骼组织各自具有关于胶原蛋白和蛋白聚糖类型以及其他细胞外基质成分和因子的广泛的组成和结构变化。组织结构组织和组成的仔细描述和定量，以及生长因子和细胞因子的定位和鉴定，是阐明肌肉骨骼完整性、损伤和修复的生物学机制的必要条件。本报告的总体目标 组织学核心（HC）是开发和利用广泛的组织学和组织形态学方法来评估肌肉骨骼组织损伤和修复，并为利用这些检测的新项目和合作提供培训和资金。具体目标是：目标1：通过正式的教育充实计划和一对一的互动，就评估肌肉骨骼组织结构和组成的各种方法的能力、优点和缺点提供指导和培训。 目的2：为骨骼肌组织的组织学和组织形态计量学研究提供专业知识和服务。目的3：开发新的基于组织学的技术，将适用于肌肉骨骼研究。目标4：为制定新项目和开展合作提供资金，并为调查人员编制初步和/或可行性数据。

PROJECT SUMMARY (See instructions): The Structure and composition of musculoskeletal tissues are tailored to meet their demanding functions. With injury, the structure and composition of these tissues deteriorate, resulting in a decline or loss of mechanical function. Musculoskeletal tissues each have a wide array of compositional and structural variety with respect to collagen and proteoglycan types, as well as other extracellular matrix constituents and factors. Careful description and quantification of tissue structural organization and composition, as well as localization and identification of growth factors and cytokines, are necessary requirements for elucidation of the biologic mechanisms underlying musculoskeletal integrity, injury, and repair. The overall objective of this Histology Core (HC) is to develop and utilize a wide range of histological and histomorphometric approaches to evaluate musculoskeletal tissue injury and repair, and to provide training and funding for new projects and collaborations utilizing these assays. The Specific Aims are: Aim 1: To provide guidance and training on the capabilities, advantages, and disadvantages of the various methodologies to assess musculoskeletal tissue structure and composition through formal educational enrichment programs and one-on-one interactions. Aim 2: To provide expertise and service for histological and histomorphometric assays of musculoskeletal tissues. Aim 3: To develop new histologically-based techniques that will be applicable to musculoskeletal research. Aim 4: To provide funding for development of new projects and collaborations and to develop preliminary and/or feasibility data for investigators.