Histology Core

组织学核心

• 批准号: 8187077
• 负责人: ROBERT JOHN PIGNOLO
• 金额: $ 12.3万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8187077
• 关键词: Address; Biological Assay; Biomechanics; Collaborations; Collagen; Core Facility; Data; Development; Disadvantaged; Environment; Extracellular Matrix; Faculty; Funding; Growth Factor; Histologic; Histological Techniques; Histology; Injury; Instruction; Mechanics; Methodology; Methods; Musculoskeletal; Musculoskeletal Diseases; Pennsylvania; Proteoglycan; Research; Research Personnel; Services; Structure; Techniques; Tissues; Training; Universities; base; cytokine; injury and repair; meetings; novel strategies; programs

PROJECT SUMMARY (See instructions): The Structure and composition of musculoskeletal tissues are tailored to meet their demanding functions. With injury, the structure and composition of these tissues deteriorate, resulting in a decline or loss of mechanical function. Musculoskeletal tissues each have a wide array of compositional and structural variety with respect to collagen and proteoglycan types, as well as other extracellular matrix constituents and factors. Careful description and quantification of tissue structural organization and composition, as well as localization and identification of growth factors and cytokines, are necessary requirements for elucidation of the biologic mechanisms underlying musculoskeletal integrity, injury, and repair. The overall objective of this Histology Core (HC) is to develop and utilize a wide range of histological and histomorphometric approaches to evaluate musculoskeletal tissue injury and repair, and to provide training and funding for new projects and collaborations utilizing these assays. The Specific Aims are: Aim 1: To provide guidance and training on the capabilities, advantages, and disadvantages of the various methodologies to assess musculoskeletal tissue structure and composition through formal educational enrichment programs and one-on-one interactions. Aim 2: To provide expertise and service for histological and histomorphometric assays of musculoskeletal tissues. Aim 3: To develop new histologically-based techniques that will be applicable to musculoskeletal research. Aim 4: To provide funding for development of new projects and collaborations and to develop preliminary and/or feasibility data for investigators.

项目总结(见说明)： 肌肉骨骼组织的结构和组成是为满足其要求的功能而量身定做的。 损伤后，这些组织的结构和成分会恶化，导致机械功能下降或丧失。每种肌肉骨骼组织在胶原和蛋白多糖类型以及其他细胞外基质成分和因子方面都有广泛的成分和结构变化。对组织结构组织和成分的详细描述和量化，以及生长因子和细胞因子的定位和鉴定，是阐明肌肉骨骼完整性、损伤和修复的生物机制的必要要求。这样做的总体目标是 组织学核心(HC)是开发和利用广泛的组织学和组织形态计量学方法来评估肌肉骨骼组织损伤和修复，并利用这些分析为新的项目和合作提供培训和资金。具体目标是：目标1：通过正式的教育充实计划和一对一的互动，就评估肌肉骨骼组织结构和组成的各种方法的能力、优缺点提供指导和培训。 目的2：为肌肉骨骼组织的组织学和组织形态计量学分析提供专业知识和服务。目的3：开发适用于肌肉骨骼研究的基于组织学的新技术。目标4：为开发新项目和合作提供资金，并为调查人员编制初步和(或)可行性数据。