transgenic and targeted mutagenesis

西北大学转基因和靶向突变（TTML）是一个共享资源，旨在 为罗伯特·H·卢里综合研究所的研究人员的研究项目生产基因工程小鼠 西北大学范伯格医学院癌症中心（RHLCCC），埃文斯顿 西北医院（ENH）和儿童纪念研究中心（CMRC）。转基因实验室 1989年，作为发育生物学马基计划的一部分，首次在CMRC组织， 通过原核显微注射产生转基因小鼠的资源。2003年，实验室搬迁至 NUFSM在Warren Tourtelevision博士的新领导下，并得到了大量大学的支持，包括 新设计/翻新的空间和新设备。今天，该设施已发展成为一个高度利用 该实验室为NU研究人员提供广泛的服务，包括转基因小鼠的产生， 胚胎干（ES）细胞的基因靶向、ES细胞显微注射到胚泡中、冷冻保存和 小鼠胚胎的恢复和无病原体小鼠品系的再衍生。自2003年以来，TTML一直是 在重组和扩大服务方面非常成功，并在产生 转基因和靶向突变小鼠。2005年，服务范围扩大到提供胚胎 冷冻保存/恢复、再衍生和体外受精。新的服务定位NU和癌症 中心的研究人员利用世界各地产生的突变小鼠的新兴储存库， 它们作为冷冻保存的胚胎储存。TTML提供了必要的基础设施， 研究人员获得转基因技术，通常需要昂贵的显微注射/组织培养 在显微注射、显微外科手术、胚胎操作、动物实验和动物实验方面拥有专业知识的设备和高技能的工作人员。 饲养和ES细胞培养。TTML工作人员提供转基因相关的各个方面的咨询 技术范围从动物方案批准到育种，筛选和分析新创建的 转基因或嵌合小鼠。此外，TTML的工作人员也可以提供有关目标选择的指导 载体设计、合适的筛选策略和基因靶向项目的DNA分离方法。的 TTML由教师监督委员会管理，该委员会每季度召开一次会议，提供有关以下方面的指导： 预算和业务问题。在过去的五年里，癌症中心的研究人员已经成为 使用TTML的NU研究人员的主要小组，强调其在整个研究使命中的关键作用 关于RHLCCC

The Northwestern University Transgenic and Targeted Mutagenesis (TTML) is a shared resource designed to produce genetically engineered mice for research projects of investigators of the Robert H Lurie Comprehensive Cancer Center (RHLCCC), Northwestern University's Feinberg School of Medicine (NUFSM), Evanston Northwestern Hospital (ENH) and Children's Memorial Research Center (CMRC). The transgenic laboratory was first organized at the CMRC in 1989 as part of the Markey Program in Developmental Biology, to provide a resource for generating transgenic mice by pronuclear microinjection. In 2003, the laboratory was moved to NUFSM under new leadership by Dr. Warren Tourtellotte and with substantial university support, including newly designed/renovated space and new equipment. Today, the facility has evolved into a highly utilized laboratory that provides a broad range of services to NU investigators, including generation of transgenic mice, gene targeting of embryonic stem (ES) cells, ES cell microinjection into blastocysts, cryopreservation and recovery of mouse embryos, and rederivation of pathogen free mouse strains. Since 2003, the TTML has been highly successful in reorganizing and expanding services, and has been highly successful in generating transgenic and targeted mutant mice. In 2005, services were expanded to offer embryo cryopreservation/recovery, rederivation and in vitro fertilization. The new services position NU and Cancer Center investigators to capitalize on emerging repositories of mutant mice generated around the world, most of which are stored as cryopreserved embryos. The TTML provides the necessary infrastructure to allow most investigators to access transgenic technology that normally requires expensive microinjection/tissue culture equipment and highly skilled staff with expertise in microinjection, microsurgeries, embryo manipulation, animal husbandry, and ES cell culture. TTML staff provide consultation on all aspects of transgenic related technologies ranging from animal protocol approval to breeding, screening and analysis of newly created transgenic or chimeric mice. Moreover, TTML staff are also available to provide guidance regarding targeting vector design, appropriate screening strategies, and DNA isolation methods for gene targeting projects. The TTML is governed by a faculty oversight committee which meets quarterly to provide guidance related to budgetary and operational issues. Over the last five years, Cancer Center investigators have become the primary group of NU investigators utilizing the TTML, emphasizing its pivotal role in the overall research mission of the RHLCCC.