TIM recognition of phosphatidylserine regulates innate adaptive immunity-Core B

TIM识别磷脂酰丝氨酸调节先天适应性免疫-Core B

• 批准号: 8831795
• 负责人: Gordon James Freeman
• 金额: $ 4.01万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8831795
• 关键词: TIM; recognition; phosphatidylserine; regulates; innate

The Scientific Core (Core B), will coordinate the use of mAbs and Ig fusion proteins for studying the expression and function of TIM proteins in T cell activation and tolerance. These reagents will be an easily accessible means of addressing the functions of these important immunoregulatory pathways. The major goals of the Scientific Core are to generate novel reagents that facilitate the analysis of the expression and function of TIM family members and to serve as a repository for and provide existing reagents for the PPG Projects. The availability of large quantities of mAbs and Ig fusion proteins Is critical to the success of Projects 1-4. MAbs will be used to visualize the expression of TIM family members and as pathway agonists and antagonists. Ig fusion proteins will be used for expression, function, and signaling studies. In addition, Ig fusion proteins may serve as a means to specifically block receptor/ligand interactions. To accomplish these goals, the Scientific Core has two aims: Specific Aim 1: To generate novel monoclonal antibodies and Ig fusion proteins to study the function and expression of TIM family members. Specific Aim 2: To serve as a repository and maintain and produce existing and newly generated TIM mAbs and Ig fusion proteins for PPG investigators. The production of these critical reagents by a core not only will be time and cost efficient, but also provide standardized reagents that will facilitate comparison of data by Investigators in this PPG.

科学核心（核心B）将协调使用mAb和IG融合蛋白研究 TIM蛋白在T细胞活化和耐受中的表达和功能。这些试剂将是解决这些重要的免疫调节途径的功能的容易获得的手段。主要 科学核心的目标是产生新的试剂，促进TIM家族成员的表达和功能的分析，并作为PPG项目的储存库和提供现有的试剂。大量mAb和IG融合蛋白的可获得性是成功的关键。 项目1-4。单克隆抗体将用于可视化TIM家族成员的表达，并作为途径激动剂和拮抗剂。IG融合蛋白将用于表达、功能和信号传导研究。此外，IG融合蛋白可作为特异性阻断受体/配体相互作用的手段。为了实现这些目标，科学核心有两个目标： 具体目的1：制备新型单克隆抗体和IG融合蛋白，研究TIM家族成员的功能和表达。 具体目标2：作为储存库，为PPG研究者维护和生产现有和新生成的TIM mAb和IG融合蛋白。 通过核心生产这些关键试剂不仅具有时间和成本效益，而且还提供了标准化试剂，便于研究者在本PPG中进行数据比较。