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NKT cells activated by apoptotie cells through TIM-1 regulate asthma

凋亡细胞通过 TIM-1 激活 NKT 细胞调节哮喘

基本信息

批准号：
7995551
负责人：
DALE T UMETSU
金额：
$ 26.7万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-09-30 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7995551
关键词：
Acute Hepatitis Affect Apoptosis Apoptotic Asthma Binding Cell Death Cell Line Cell physiology Cells Development Disease Environment Epithelial Cells General Population Genetic Polymorphism Goals Grant Hepatic Hepatitis Hepatitis A Virus Hepatocyte Human Hypersensitivity Immune Inbred BALB C Mice Inflammation Injury Lead Liver Liver diseases Lung Macaca mulatta Mediating Modeling Modification Molecular Monkeys Mouse Strains Mus Oxidative Stress Pathogenesis Pathway interactions Pattern Pattern Recognition Pattern recognition receptor Production Public Health Reagent Regulation Severities Signal Transduction Susceptibility Gene T-Cell Activation Testing Virus Diseases adaptive immunity airway hyperresponsiveness airway inflammation atopy congenic cytokine cytotoxic eosinophil in vitro Model in vivo in vivo Model insight killer T cell novel ozone exposure pathogen phosphatidylserine receptor receptor response

项目摘要

The long-term goals of Project 1 are to determine how TIM-1, which binds phsophatidylserine (PtdSer) on apoptotic cells, regulates the development of asthma. In the previous grant period, we showed that TIMl is an important atopy susceptibility gene interacting with the envirormient, and that TIM-1 is an important receptor for PtdSer. In the next grant period, we will extend these observations, and hypothesize that asthma is regulated by Natural Killer T (NKT) cells expressing TIM-1, and activated by apoptotic bronchial epithelial cells expressing PtdSer. We will show that NKT cells activated by apoptotic cells in the airways can amplify innate and adaptive immunity, and cause airway hyperreactivity (AHR), a cardinal feature of asthma. In Specific Aim 1, we propose to clarify the mechanisms by which TIM-1 costimulates the activation of NKT cells. We will demonstrate that apoptotic cells bind to and activate NKT cells, in a TIM-1 and PtdSer specific manner. Moreover, we will show that such a pathway can occur in vivo, by establishing an in vivo model in which liver cells, made apoptotic by treatment with anti-Fas mAb, activate NKT cells, which are present in large numbers in the liver. In Specific Aim 2, we hypothesize that the hepatitis A virus (HAV), by binding to TIM-1 on human NKT cells, can activate the NKT cells. We will show that that HAV-activated NKT cells are cytotoxic for hepatocytes, and suggest that these HAV-activated NKT cells later protect against the development of asthma. We will also show that TIMl, a susceptibility gene for asthma, is also a susceptibility gene for severe HAV infection and hepatitis. Moreover, by examining a monkey model of HAV infection, we will demonstrate that HAV infection is indeed associated with the activation and expansion of hepatic NKT cells. In Specific Aim 3, we will examine how NKT cells, responding through TIM-1 to apoptotic airway epithelial cells in the lung, mediate AHR. We hypothesize that oxidative stress in the airways causes airway epithelial cell apoptosis, which can then activate NKT cells, resulting in the development of AHR. Our studies will be facilitated by unique reagents, including primary NKT cell lines, activating and blocking anti-TIM-1 mAb, TIM-1 Tg mice, TIM -/- mice, TIM-3-/- mice, Nrf2 -/- mice and Rhesus monkeys. These studies will provide significant insight into an important human atopy susceptibility gene (TIMl). Moreover, we will demonstrate that TIM-1 on NKT cells functions as a pattern recognition receptor that senses PtdSer as a DAMP (damage associated molecular pattern), and that apoptotic bronchial epithelial cells provide a "danger" signal that activates NKT cells, profoundly affecting airway inflammation and asthma.

项目1的长期目标是确定TIM-1如何调节哮喘的发展，TIM-1结合凋亡细胞上的磷脂酰丝氨酸（PtdSer）。在之前的研究中，我们发现TIMl是一个重要的与环境相互作用的特应性易感基因，而TIM-1是PtdSer的一个重要受体。在下一个资助期，我们将扩展这些观察结果，并假设哮喘由表达TIM-1的自然杀伤T细胞（NKT）调节，并由表达PtdSer的凋亡支气管上皮细胞激活。我们将证明气道中凋亡细胞激活的NKT细胞可以增强先天和适应性免疫，并引起气道高反应性（AHR），这是哮喘的主要特征。