The properties and evolvability of the cyanobacterial circadian genetic network
The properties and evolvability of the cyanobacterial circadian genetic network
批准号:
8456352
负责人:
Ryan K Shultzaberger
金额:
$5.39万
依托单位国家:
美国
项目类别:
财政年份:
2012
资助国家:
美国
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31
关键词:
AffectBacteriaBiochemicalBiological AssayBiological ProcessCircadian RhythmsCloningComputersCore ProteinCyanobacteriumDNA SequenceGene ExpressionGene Expression RegulationGenesGeneticHourHumanIn VitroIndividualKnock-outLibrariesLightLuciferasesMeasuresMolecularOrganismOther GeneticsOutputPathologyPharmaceutical PreparationsPhenotypePhosphorylationPropertyProteinsRandomizedRegulationRelative (related person)ResistanceSourceSynechococcusSystemVariantcharge coupled device camerafitnessgene interactiongenetic evolutioninsightknockout geneluminescencemutantpressurepromoterreconstitutionresearch study
中文摘要
描述(由申请人提供):我将探索蓝藻长聚球菌PCC 7942中昼夜节律遗传网络的特性和进化,以更好地了解昼夜节律调节的分子机制,其可塑性(可进化性)和基因网络的一般特性。为此,我提出了三组实验。(1)系统地、具体地改变长叶螺昼夜节律遗传网络中的参数(即时钟基因的丰度、稳定性和生化特性),量化昼夜节律的差异。这将提供一种自下而上的方法来确定这些参数对表型的相对贡献,网络对这些参数扰动的鲁棒性,以及通过相对简单的改变可以推动系统具有的表型范围。(2)确定数千个单基因和双基因敲除的昼夜周期,以确定影响昼夜周期的基因数量和程度。(3)在不同的光照-黑暗周期中,通过选择实验进化出具有改变昼夜节律的长尾螺,直接探索昼夜节律遗传网络的可进化性。这里的见解将有助于我们理解
英文摘要
DESCRIPTION (provided by applicant): I will probe the properties and evolution of the genetic network that underlies circadian rhythm in the cyanobacteria Synechococcus elongatus PCC 7942 to better understand the molecular mechanism of circadian regulation, its plasticity (evolvability) and general properties of gene networks. To do this, I propose three sets of experiments. (1) Systematically and specifically vary parameters within the circadian genetic network in S. Elongatus (i.e. abundance, stability and biochemical properties of clock genes) and quantify differences in circadian rhythms. This will provide a bottom-up approach to determine the relative contribution of these parameters to phenotype, the robustness of the network to perturbations in these parameters, and the range of phenotypes that the system can be pushed to have with relatively simple changes. (2) Determine the circadian period of thousands of single and double gene knockouts to determine the number of genes that influence circadian period and to what extent. (3) Experimentally evolve S. elongatus to have altered circadian rhythms by selection in different light-dark cycles to directly probe the evolvability of the circadian genetic network. Insights here will be useful in our understanding of
circadian control and its pathologies in higher organisms, as well as our understanding of other genetic networks.
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会议论文
The properties and evolvability of the cyanobacterial circadian genetic network
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批准号:8254581
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项目类别:
-
资助金额:$5.22万
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财政年份:2012
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负责人:Ryan K Shultzaberger
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依托单位:
国内基金
海外基金
Segmented Filamentous Bacteria激活宿主免疫系统抑制其拮抗菌 Enterobacteriaceae维持菌群平衡及其机制研究
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批准号:81971557
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项目类别:面上项目
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资助金额:65.0万元
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批准年份:2019
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负责人:毛开睿
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依托单位:
电缆细菌(Cable bacteria)对水体沉积物有机污染的响应与调控机制
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批准号:51678163
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项目类别:面上项目
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资助金额:64.0万元
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批准年份:2016
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负责人:许玫英
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依托单位: