Mechanisms of epidermal growth during development, homeostasis, and tumorigenesis

发育、稳态和肿瘤发生过程中表皮生长的机制

• 批准号: 8726283
• 负责人: Slobodan Beronja
• 金额: $ 22.19万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8726283
• 关键词: Academia; Address; Adult; Behavior; Bioinformatics; Biological; Biological Process; Biology; Biometry; Cancer Biology; Candidate Disease Gene; Cells; Clinical Research; Complex; Computational Biology; Development; Developmental Biology; Disease; Educational workshop; Embryo; Ensure; Environment; Epithelial; Equipment; Foundations; Funding Agency; Genes; Goals; Grant; Growth; Health; Homeostasis; Human; Human Development; Hyperplasia; In Vitro; Instruction; Investigation; Laboratories; Malignant Neoplasms; Mediating; Memorial Sloan-Kettering Cancer Center; Methods; Modeling; Molecular; Mus; Mutation; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Nature; Neonatal; Oncogenes; Oncogenic; Organ; Organism; Pathway interactions; Physiological; Positioning Attribute; Process; RNA Interference; Regulation; Research; Research Personnel; Resources; Role; Scientist; Skin; Solid; Staging; System; Tissues; Training; United States National Institutes of Health; Universities; Work; anticancer research; base; career; career development; design; experience; follow-up; gene function; genome wide association study; genome-wide; in vivo; insight; interest; lectures; medical schools; meetings; novel; originality; post-doctoral training; programs; repaired; research and development; screening; self-renewal; skills; therapeutic target; tumor; tumorigenesis

Project Summary Throughout the graduate and postdoctoral stages, my goal has been to get training and experience that will allow me to establish an independent and successful research program in developmental and cancer biology. Specifically, I am interest in the mechanisms that regulate epithelial tissue development and homeostasis, and to changes that occur in disease states such as cancer. My plan for the reminder of the postdoctoral training is to acquire additional skills and develop research material that I will be able to carry into an independent position in academia. The Rockefeller University is an ideal place for the development of my career as an independent researcher. The University is a part of the Tri-Institutional Program, together with Memorial Sloan-Kettering Cancer Center and Weill-Cornell Medical College, which is a premier environment to conduct research, interact with fellow scientists, and attend lectures by leaders in a variety of fields. The Tri-Institutional group also organizes workshops in bioinformatics and biostatistics, and lectures dedicated to clinical and cancer research, both of which are the focus of my training. Furthermore, here I will interact with ollaborators that are experts in computational and cancer biology. Lastly, the resources within the Fuchs laboratory and the Resource Centers present at the Rockefeller University will provide equipment, training, and technical help that will ensure successful completion of the proposed research. In order to meet my research and career development goals I propose to conduct a genome-wide, RNAi-mediated modifier screen in vivo, to uncover regulatory mechanisms that govern epidermal tissue growth and homeostasis during development and in oncogene-induced hyperplasia. I expect that this comprehensive work will result in identification of many candidate genes that specifically operate within the physiological environment. The high-throughput nature and the originality of the approach that I propose, will allow me to complete postdoctoral training, and in the process set up a solid foundation for the future research program. At the start of the independent stage, I will base my research on identifying mechanisms used by selected candidates, to regulate tissue growth in the models of embryonic skin development and epidermal tumorigenesis. I will use these findings in subsequent grant support applications to the NIH (NIAMS and NCI), and other available sources of funding.

项目摘要 在整个研究生和博士后阶段，我的目标是获得培训和经验， 请允许我在发育和癌症生物学方面建立一个独立和成功的研究计划。 具体来说，我对调节上皮组织发育和稳态的机制感兴趣， 发生在疾病状态如癌症中的变化。为了提醒博士后培训，我的计划是获得额外的技能并开发研究材料，以便我能够将其带入学术界的独立职位。 洛克菲勒大学是一个理想的地方，为我的职业发展作为一个独立的 研究员该大学是三机构计划的一部分，连同纪念斯隆-凯特琳癌症中心和威尔-康奈尔医学院，这是一个首要的环境进行研究，与同行的科学家互动，并参加讲座的领导人在各个领域。三机构小组还组织生物信息学和生物统计学的研讨会，以及致力于临床和癌症研究的讲座，这两者都是我培训的重点。此外，在这里，我将与计算和癌症生物学方面的专家进行互动。最后，福克斯实验室和洛克菲勒大学资源中心的资源将提供设备、培训和技术帮助， 成功完成了所提出的研究。 为了满足我的研究和职业发展目标，我建议进行全基因组，RNAi介导的修饰剂筛选体内，揭示调控机制，支配表皮组织生长和稳态在发展过程中，并在癌基因诱导的增生。我希望这项全面的工作 将导致鉴定出许多在生理环境中特异性操作的候选基因。我所提出的方法的高通量性质和独创性将使我能够完成博士后培训，并在此过程中为未来的研究计划奠定坚实的基础。在独立阶段的开始，我将基于我的研究，确定所选择的 候选人，以调节胚胎皮肤发育和表皮肿瘤发生模型中的组织生长。我将在随后向NIH（NIAMS和NCI）和其他可用资金来源申请资助时使用这些发现。