Mechanisms of epidermal growth during development, homeostasis, and tumorigenesis

项目摘要 在整个研究生和博士后阶段，我的目标一直是获得培训和经验，这将 请允许我在发育和癌症生物学方面建立一个独立和成功的研究计划。 具体地说，我对调节上皮组织发育和动态平衡的机制感兴趣，以及 在癌症等疾病状态下发生的变化。我的博士后培训计划是为了获得更多的技能，并开发研究材料，使我能够在学术界担任独立的职位。 洛克菲勒大学是我发展独立职业生涯的理想场所 研究员。该大学与纪念斯隆-凯特琳癌症中心和威尔-康奈尔医学院一起，是三所院校计划的一部分，这是进行研究、与科学家同行互动并参加不同领域领导人讲座的主要环境。三机构小组还组织生物信息学和生物统计学的研讨会，以及致力于临床和癌症研究的讲座，这两个都是我培训的重点。此外，在这里，我将与计算和癌症生物学方面的专家进行互动。最后，Fuchs实验室和洛克菲勒大学的资源中心将提供设备、培训和技术帮助，以确保 圆满完成了拟议的研究。 为了实现我的研究和职业发展目标，我提议在体内进行全基因组RNAi介导的修饰物筛选，以揭示在发育过程中和癌基因诱导的增殖过程中控制表皮组织生长和动态平衡的调节机制。我期待着这项全面的工作 将导致识别许多在生理环境中特定操作的候选基因。我提出的方法的高通量性质和独创性，将使我能够完成博士后培训，并在此过程中为未来的研究计划奠定坚实的基础。在独立阶段的开始阶段，我的研究将基于确定选定的 候选，在胚胎皮肤发育和表皮肿瘤形成的模型中调节组织生长。我将在随后向NIH(NIAMS和NCI)以及其他可用的资金来源提出的赠款支持申请中使用这些发现。

Project Summary Throughout the graduate and postdoctoral stages, my goal has been to get training and experience that will allow me to establish an independent and successful research program in developmental and cancer biology. Specifically, I am interest in the mechanisms that regulate epithelial tissue development and homeostasis, and to changes that occur in disease states such as cancer. My plan for the reminder of the postdoctoral training is to acquire additional skills and develop research material that I will be able to carry into an independent position in academia. The Rockefeller University is an ideal place for the development of my career as an independent researcher. The University is a part of the Tri-Institutional Program, together with Memorial Sloan-Kettering Cancer Center and Weill-Cornell Medical College, which is a premier environment to conduct research, interact with fellow scientists, and attend lectures by leaders in a variety of fields. The Tri-Institutional group also organizes workshops in bioinformatics and biostatistics, and lectures dedicated to clinical and cancer research, both of which are the focus of my training. Furthermore, here I will interact with ollaborators that are experts in computational and cancer biology. Lastly, the resources within the Fuchs laboratory and the Resource Centers present at the Rockefeller University will provide equipment, training, and technical help that will ensure successful completion of the proposed research. In order to meet my research and career development goals I propose to conduct a genome-wide, RNAi-mediated modifier screen in vivo, to uncover regulatory mechanisms that govern epidermal tissue growth and homeostasis during development and in oncogene-induced hyperplasia. I expect that this comprehensive work will result in identification of many candidate genes that specifically operate within the physiological environment. The high-throughput nature and the originality of the approach that I propose, will allow me to complete postdoctoral training, and in the process set up a solid foundation for the future research program. At the start of the independent stage, I will base my research on identifying mechanisms used by selected candidates, to regulate tissue growth in the models of embryonic skin development and epidermal tumorigenesis. I will use these findings in subsequent grant support applications to the NIH (NIAMS and NCI), and other available sources of funding.