Development of a novel highly effective influenza vaccine
新型高效流感疫苗的研制
基本信息
- 批准号:8781471
- 负责人:
- 金额:$ 30万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-06-15 至 2016-05-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAdultAntibodiesAntibody FormationAttenuated Live Virus VaccineB-LymphocytesBeliefBody Weight decreasedCategoriesCell Culture SystemCellsCessation of lifeChildComplementDataDevelopmentDisadvantagedEffectivenessElderlyEpitopesEvaluationFlu virusGenerationsGenesGoalsHealthHistologyHospitalizationHumanImmune responseImmunityImmunocompromised HostIndividualInfectionInflammatory ResponseInfluenzaInfluenza A Virus, H1N1 SubtypeInfluenza A Virus, H5N1 SubtypeInfluenza A Virus, H7N9 SubtypeLifeLongevityLungMediatingMeta-AnalysisMethodsMucosal Immune ResponsesMusNational Institute of Allergy and Infectious DiseasePathologyPopulationProductionPropertyPublic HealthRiskRoleSafetySeasonsSolidT cell responseT memory cellT-LymphocyteTestingTimeLineUncertaintyVaccinatedVaccinationVaccinesVertebral columnViralViral AntigensViral GenomeViral ProteinsVirionVirusVirus SheddingVulnerable PopulationsWheezingagedbasecell typecytokineeggeosinophilfluin vivoinfluenza virus vaccineinfluenzavirusmeetingsmortalityneutrophilnovelnovel vaccinespandemic influenzapathogenphase 2 studypre-clinicalpublic health relevancerapid techniqueresearch clinical testingresponseseasonal influenzavaccine safety
项目摘要
DESCRIPTION (provided by applicant): Seasonal influenza (flu) virus, an NIAID category C priority pathogen, causes widespread infection, resulting in at least 3-5 million cases of severe illness and 250,000-500,000 deaths worldwide. Young children and elderly or immunocompromised individuals are typically at greater risk of severe illness or death from influenza. Newly emerging strains can result in influenza pandemics with much higher mortality rates, even in young healthy adults. To address this threat to public health, annual universal vaccination is recommended for all individuals aged over 6 months in the US. Current vaccines include inactivated trivalent split or subunit and live attenuated vaccine, both of which have the drawback that they must be grown using laborious methods in eggs and reformulated every year based on the influenza strains predicted to be prevalent in the next flu season. However, the major disadvantage of these vaccines is a surprising lack of effectiveness, which was highlighted in a recent meta analysis of influenza vaccine (live and inactivated) in the US. Even in the recent 2012-13 season in which the vaccine was well-matched to circulating strains, only 59% efficacy across the population and a meager 9% efficacy in the elderly was achieved, casting doubt on the long-standing belief that a close match between the vaccine virus strains and circulating strains results in high effectiveness. There is an urgent need for the development of highly effective and cross-protective influenza vaccines and new rapid methods of manufacturing. To meet this need FluGen has developed a novel vaccine virus (M2SR) based on the deletion of the M2 gene. This deletion in the viral genome allows for single replication of the vaccine virus in the host and production of viral proteins, which induces strong cross-protective immunity without the generation of progeny virions (shedding), a goal unmet by current vaccine strategies. The M2SR is a platform backbone virus that can be modified to encode the viral antigens from any influenza strain and is produced in a novel cell culture system, avoiding the use of eggs. We hypothesize that M2SR will provide safe, highly effective, broad spectrum, long-lasting protection against influenza. Our preliminary data support this hypothesis and show that the vaccine elicits strong systemic and mucosal immune responses and provides effective cross-reactive protection against lethal challenge with influenza. We will test this hypothesis in 3 Specific Aims: Aim 1. To determine the efficacy of protection afforded by the M2SR vaccine. We will further investigate the efficacy and longevity of protection against homologous and heterologous viral challenge. Aim 2. To determine whether M2SR has any pathological effects. Lung histology and the inflammatory response will be assessed after vaccination and challenge. Aim 3. To determine the mechanism of heterologous protection. We will investigate the role of virus-specific T and B cell responses in cross-protection. These studies will provide a comprehensive pre-clinical evaluation of the efficacy and safety of the M2SR vaccine.
描述(申请人提供):季节性流感(流感)病毒是NIAID C类优先病原体,可引起广泛感染,导致全球至少300-500万重症病例和250,000-500,000人死亡。幼儿和老年人或免疫功能受损的人患严重疾病或死于流感的风险通常更大。新出现的毒株可能导致死亡率高得多的流感大流行,即使在年轻的健康成年人中也是如此。为了应对这种对公共健康的威胁,建议美国所有6个月以上的儿童每年普遍接种疫苗。目前的疫苗包括灭活的三价裂解或亚单位疫苗和减毒活疫苗,这两种疫苗的缺点都是必须使用繁琐的方法在鸡蛋中培养,并根据预计将在下一个流感季节流行的流感毒株每年重新配制疫苗。然而,这些疫苗的主要缺点是令人惊讶地缺乏有效性,这在最近对美国流感疫苗(活的和灭活的)的荟萃分析中得到了强调。即使在最近的2012-13年疫苗与流行毒株很好匹配的季节,也只有59%的人群和9%的老年人实现了疗效,这让人对长期以来的信念产生了怀疑,即疫苗病毒毒株和传播毒株之间的紧密匹配会带来高效。迫切需要开发高效和交叉保护的流感疫苗和新的快速制造方法。为了满足这一需求,FluGen开发了一种基于M2基因缺失的新型疫苗病毒(M2SR)。病毒基因组的这种缺失允许疫苗病毒在宿主中的单一复制和病毒蛋白的产生,从而在不产生后代病毒粒子(脱落)的情况下诱导强大的交叉保护免疫,这是当前疫苗策略无法实现的目标。M2SR是一种平台主干病毒,可以修改为编码任何流感病毒株的病毒抗原,并在一种新的细胞培养系统中生产,避免使用鸡蛋。我们假设M2SR将对流感提供安全、高效、广谱、持久的保护。我们的初步数据支持这一假设,并表明该疫苗可引发强烈的系统和粘膜免疫反应,并提供有效的交叉反应保护,以抵御流感的致命攻击。我们将在3个具体目标上验证这一假设:目的1.确定M2SR疫苗提供的保护效果。我们将进一步研究针对同源和异种病毒攻击的保护效果和寿命。目的2.确定M2SR是否具有病理效应。接种和激发后将对肺组织学和炎症反应进行评估。目的3.确定异源保护的机制。我们将研究病毒特异性T和B细胞反应在交叉保护中的作用。这些研究将对M2SR疫苗的有效性和安全性进行全面的临床前评估。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Pamuk Bilsel其他文献
Pamuk Bilsel的其他文献
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{{ truncateString('Pamuk Bilsel', 18)}}的其他基金
Safety and Immunogenicity of H3N2 M2SR monovalent influenza vaccine in older subjects
H3N2 M2SR 单价流感疫苗在老年受试者中的安全性和免疫原性
- 批准号:
10436972 - 财政年份:2020
- 资助金额:
$ 30万 - 项目类别:
Safety and Immunogenicity of H3N2 M2SR monovalent influenza vaccine in older subjects
H3N2 M2SR 单价流感疫苗在老年受试者中的安全性和免疫原性
- 批准号:
10246781 - 财政年份:2020
- 资助金额:
$ 30万 - 项目类别:
IND-enabling studies of an intranasal, single-replication M2SR influenza vaccine
鼻内单复制 M2SR 流感疫苗的 IND 研究
- 批准号:
10697911 - 财政年份:2015
- 资助金额:
$ 30万 - 项目类别:
Restimulating memory T cell responses in elderly by a novel, live influenza vaccine
通过新型活流感疫苗重新刺激老年人的记忆 T 细胞反应
- 批准号:
9408434 - 财政年份:2015
- 资助金额:
$ 30万 - 项目类别:
Development of a novel highly effective influenza vaccine
新型高效流感疫苗的研制
- 批准号:
8868029 - 财政年份:2014
- 资助金额:
$ 30万 - 项目类别:
A high-growth PR8 virus for pandemic vaccine production in ST6-Vero cells
用于在 ST6-Vero 细胞中生产大流行疫苗的高生长 PR8 病毒
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8251012 - 财政年份:2012
- 资助金额:
$ 30万 - 项目类别:
High-Expression, Rapid Production of Influenza Vaccines in Cell-Based Systems
在细胞系统中高表达、快速生产流感疫苗
- 批准号:
8517004 - 财政年份:2011
- 资助金额:
$ 30万 - 项目类别:
High-Expression, Rapid Production of Influenza Vaccines in Cell-Based Systems
在细胞系统中高表达、快速生产流感疫苗
- 批准号:
8075922 - 财政年份:2011
- 资助金额:
$ 30万 - 项目类别:
High-Expression, Rapid Production of Influenza Vaccines in Cell-Based Systems
在细胞系统中高表达、快速生产流感疫苗
- 批准号:
8321463 - 财政年份:2011
- 资助金额:
$ 30万 - 项目类别:
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