Bioengineering Tracheas Through Targeting Activated CD47

描述（由申请人提供）：气管丧失是一种毁灭性的疾病，气管仍然是剩余的重要器官之一，没有足够的替代品。从合成构建物或脱细胞气管支架工程化气管移植物的尝试已经取得了有限的成功，包括长期和充满并发症的继发性愈合和灾难性的气管管腔塌陷。这项提案将调查新发现的信号通路和它们在限制细胞和组织再生，移植和气管血管生成中的作用。从我们独特的小鼠原位气管移植模型的实验中获得的知识将转化为产生功能性的“铲准备”的人气管。

DESCRIPTION (provided by applicant): Tracheal loss is a devastating condition and the trachea remains one of the remaining vital organs for which there is no adequate substitute. Attempts at engineering tracheal transplants from either synthetic constructs or decellularized tracheal scaffolds have met with limited success including long and complication-filled secondary healing and catastrophic tracheal luminal collapse. This proposal will investigate newly identified signaling pathways and the role that they play in limiting cellular and tissue regeneration, engraftment and tracheal angiogenesis. Knowledge gained from experiments in our unique murine model of orthotopic tracheal transplant will be translated into generating a functional "shovel-ready" human trachea.