Yeast 2 Hybrid

酵母2杂交

• 批准号: 8725205
• 负责人: Sergio R. Padilla-Lopez
• 金额: $ 16.81万
• 依托单位: SANFORD RESEARCH/USD
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8725205
• 关键词: Biomedical Research; Centers of Research Excellence; Child health care; Communities; Complex; Consult; Custom; Detection; Disease; Equipment; Faculty; Fee-for-Service Plans; Fees; Funding; Goals; Homeostasis; Housing; Human Resources; Hybrids; Individual; Institutes; Instruction; Laboratories; Laboratory Research; Maintenance; Manuscripts; Membrane Proteins; Methods; Pediatric Research; Preparation; Production; Protein Binding Domain; Proteins; Protocols documentation; Regulation; Research; Research Institute; Research Personnel; Research Project Grants; Resource Sharing; Resources; Scientist; Services; Signaling Protein; Site; Structure; System; Technical Expertise; Techniques; Training; Translational Research; United States National Institutes of Health; Work; Yeasts; base; cDNA Library; cost; design; follow-up; insight; interest; meetings; novel; protein protein interaction; research study; response; screening; therapeutic target; web site; yeast genetics

PROJECT SUMMARY (See instructions): The Yeast 2-Hybrid Core will provide support and expertise in detection of novel protein-protein interactions through Yeast 2-Hybrid screening for the COBRE research Project Leaders, as well as other faculty both at Sanford Research/USD and at regional research institutes. The core will provide several different approaches for Yeast 2-Hybrid screening, allowing for the design of a unique, customized method for screening protein-protein interactions that best meet the needs of each individual project. By centralizing these Yeast 2-Hybrid services, the core will minimize labor and cost to individual laboratories interested in performing screens as well as bring forth the yeast genetics expertise of the core director to expedite troubleshooting or manipulation of screening systems to better meet their needs. The purpose of this core is to support the goals of the COBRE Project Leaders and other research scientist seeking to uncover novel protein-protein signaling complexes.

项目摘要（参见说明）： Yeast 2-Hybrid Core 将通过 Yeast 2-Hybrid 筛选为 COBRE 研究项目负责人以及 Sanford Research/USD 和地区研究机构的其他教员提供支持和专业知识，以检测新型蛋白质-蛋白质相互作用。该核心将为酵母 2-Hybrid 筛选提供几种不同的方法，允许设计独特的定制方法来筛选最能满足每个项目需求的蛋白质-蛋白质相互作用。通过集中这些酵母 2-Hybrid 服务，该核心将最大限度地减少有兴趣进行筛选的各个实验室的劳动力和成本，并发挥核心主任的酵母遗传学专业知识，以加快筛选系统的故障排除或操作，以更好地满足他们的需求。该核心的目的是支持 COBRE 项目领导者和其他研究科学家寻求发现新型蛋白质-蛋白质信号复合物的目标。