Yeast 2 Hybrid

酵母2杂交

• 批准号: 9335896
• 负责人: Sergio R. Padilla-Lopez
• 金额: $ 16.81万
• 依托单位: SANFORD RESEARCH/USD
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9335896
• 关键词: Biomedical Research; Centers of Research Excellence; Child health care; Communities; Complex; Consult; Custom; Detection; Disease; Equipment; Faculty; Fee-for-Service Plans; Fees; Funding; Goals; Homeostasis; Human Resources; Hybrids; Individual; Institutes; Laboratories; Laboratory Research; Maintenance; Manuscripts; Membrane Proteins; Methods; Pathogenicity; Pediatric Research; Preparation; Production; Protein Binding Domain; Proteins; Protocols documentation; Regulation; Research; Research Institute; Research Personnel; Research Project Grants; Resource Sharing; Resources; Scientist; Services; Signaling Protein; Site; Structure; System; Technical Expertise; Techniques; Training; Translational Research; United States National Institutes of Health; Yeasts; cDNA Library; cost; design; experimental study; follow-up; insight; interest; novel; protein protein interaction; response; screening; therapeutic target; web site; yeast genetics

PROJECT SUMMARY (See instructions): The Yeast 2-Hybrid Core will provide support and expertise in detection of novel protein-protein interactions through Yeast 2-Hybrid screening for the COBRE research Project Leaders, as well as other faculty both at Sanford Research/USD and at regional research institutes. The core will provide several different approaches for Yeast 2-Hybrid screening, allowing for the design of a unique, customized method for screening protein-protein interactions that best meet the needs of each individual project. By centralizing these Yeast 2-Hybrid services, the core will minimize labor and cost to individual laboratories interested in performing screens as well as bring forth the yeast genetics expertise of the core director to expedite troubleshooting or manipulation of screening systems to better meet their needs. The purpose of this core is to support the goals of the COBRE Project Leaders and other research scientist seeking to uncover novel protein-protein signaling complexes.

项目总结（见说明）： 酵母双杂交核心将通过酵母双杂交筛选为COBRE研究项目负责人以及Sanford Research/USD和区域研究机构的其他教师提供检测新型蛋白质-蛋白质相互作用的支持和专业知识。核心将为酵母双杂交筛选提供几种不同的方法，允许设计一种独特的，定制的方法来筛选最能满足每个项目需求的蛋白质-蛋白质相互作用。通过集中这些Yeast 2-Hybrid服务，核心将最大限度地减少对执行筛选感兴趣的各个实验室的劳动力和成本，并带来核心总监的酵母遗传学专业知识，以加快筛选系统的故障排除或操作，以更好地满足他们的需求。该核心的目的是支持COBRE项目领导人和其他研究科学家寻求发现新的蛋白质-蛋白质信号复合物的目标。