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A Pixel Array Detector System for Small Angle X-ray Scattering

小角X射线散射像素阵列探测器系统

基本信息

批准号：
8734887
负责人：
Scott Classen
金额：
$ 99.76万
依托单位：
UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-18 至 2016-09-17
项目状态：
已结题

项目摘要

Project Summary Your protein doesn't crystallize, and your best postdocs can't concentrate it greater than ~1 mg/mL. Structural details are key to unlocking the protein's complex role in human biology. What do you do? Like thousands of other biology researchers have each year, you turn to Small Angle X-ray Scattering (SAXS). Unfortunately, 1 mg/mL is too dilute to achieve a reasonable level of detail from SAXS experiments. Fortunately, new zero-background pixel array photon- counting X-ray detectors are pushing these boundaries lower. If such a detector were available at the SIBYLS beamline, an accessible synchrotron SAXS facility, your troublesome protein could fuel your next manuscript, grant, or presentation. The SIBYLS beamline is a world class synchrotron resource for thousands of structural biologists located at the Advanced Light Source (ALS), Lawrence Berkeley National Lab. The beamline consists of two interchangeable endstations that enable researchers to perform both conventional macromolecular crystallography and SAXS experiments. SIBYLS has the only biological SAXS endstation at the ALS and is heavily subscribed, supporting a wide variety of scientists who study DNA repair, cancer, aging, immunology, virology, photosynthesis, and structural genomics. Scientists and staff at SIBYLS have implemented high throughput capabilities for SAXS data collection that have greatly impacted productivity (as measured by publication number and quality). The scientists and staff of SIBYLS have successfully provided not only a top-notch SAXS facility, but also developed new software and methods to improve SAXS data collection, processing, and analysis. As those achievements prove, our original MarCCD 165 detector (Rayonix) has performed admirably over the past seven years, but it is no longer under a service contract and is ineligible for renewal. Repairs are difficult as Rayonix no longer manufactures spare parts. Moreover, our current CCD-based detector has been rendered obsolete with the advent of modern zero- background photon counting detector technology that is better suited for SAXS data collection. We therefore propose to replace our current system with a PILATUS3 2M detector manufactured by DECTRIS. This proposal justifies our choice of detector, its integration with the existing data collection software, and the importance of the NIH-funded science that it will support. A modern detector for the SIBYLS SAXS endstation will leverage the significant investment already made in biological solution scattering at the ALS and, as a result, will rejuvenate this valuable resource for all NIH-funded researchers for years to come.

项目概要你的蛋白质不会结晶，你最好的博士后也不能将其浓缩到大于~1 毫克/毫升。结构细节是解开该蛋白质在人类生物学中复杂作用的关键。你做什么工作？就像每年成千上万的其他生物学研究人员一样，您求助于 Small 角 X 射线散射 (SAXS)。不幸的是，1 mg/mL 太稀，无法达到合理的浓度 SAXS 实验的详细程度。幸运的是，新的零背景像素阵列光子计算 X 射线探测器正在降低这些界限。如果有这样的探测器在 SIBYLS 光束线，一个易于使用的同步加速器 SAXS 设施，您的麻烦蛋白质可以为您的下一份手稿、资助或演示提供动力。 SIBYLS 光束线是世界一流的同步加速器资源，可用于数千个结构位于劳伦斯伯克利国家实验室先进光源 (ALS) 的生物学家。这光束线由两个可互换的终端站组成，使研究人员能够执行常规大分子晶体学和 SAXS 实验。 SIBYLS 拥有唯一 ALS 上的生物 SAXS 终端站得到了大量订阅，支持多种研究 DNA 修复、癌症、衰老、免疫学、病毒学、光合作用和结构基因组学。 SIBYLS 的科学家和工作人员实现了高通量 SAXS 数据收集功能极大地影响了生产力（通过测量出版数量和质量）。 SIBYLS 的科学家和工作人员已成功提供不仅拥有一流的 SAXS 设施，还开发了新的软件和方法来改进 SAXS 数据收集、处理和分析。正如这些成就所证明的那样，我们原创的 MarCCD 165 探测器 (Rayonix) 已经实现了过去七年表现出色，但已不再符合服务合同且不符合资格用于续订。由于 Rayonix 不再生产备件，因此维修很困难。此外，我们的随着现代零探测器的出现，当前基于 CCD 的探测器已被淘汰。背景光子计数探测器技术更适合 SAXS 数据收集。因此，我们建议用 PILATUS3 2M 探测器替换我们当前的系统由 DECTRIS 制造。该提案证明了我们选择探测器及其与现有的数据收集软件，以及 NIH 资助的科学的重要性，它将支持。 SIBYLS SAXS 终端站的现代探测器将利用重要的已经对 ALS 的生物溶液分散进行了投资，因此，将在未来几年为所有 NIH 资助的研究人员恢复这一宝贵资源。

项目成果

期刊论文数量（17）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Supramolecular Peptoid Structure Strengthens Complexation with Polyacrylic Acid Microgels.

超分子肽结构增强与聚丙烯酸微凝胶的络合。

DOI：
10.1021/acs.biomac.3c01242
发表时间：
2024
期刊：
Biomacromolecules
影响因子：
6.2
作者：
Zhao,Wenhan;Lin,JenniferS;Nielsen,JosefineEilsø;Sørensen,Kristian;Wadurkar,AnandSunil;Ji,Jingjing;Barron,AnneliseE;Nangia,Shikha;Libera,MatthewR
通讯作者：
Libera,MatthewR

A potent and durable malaria transmission-blocking vaccine designed from a single-component 60-copy Pfs230D1 nanoparticle.

DOI：
10.1038/s41541-023-00709-8
发表时间：
2023-08-18
期刊：
NPJ VACCINES
影响因子：
9.2
作者：
Salinas, Nichole D.;Ma, Rui;Dickey, Thayne H.;McAleese, Holly;Ouahes, Tarik;Long, Carole A.;Miura, Kazutoyo;Lambert, Lynn E.;Tolia, Niraj H.
通讯作者：
Tolia, Niraj H.

The BAM7 gene in Zea mays encodes a protein with similar structural and catalytic properties to Arabidopsis BAM2

DOI：
10.1107/s2059798322002169
发表时间：
2022-05-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
影响因子：
2.2
作者：
Ravenburg, Claire M.;Riney, McKayla B.;Berndsen, Christopher E.
通讯作者：
Berndsen, Christopher E.

Self-assembly and regulation of protein cages from pre-organised coiled-coil modules.

DOI：
10.1038/s41467-021-21184-6
发表时间：
2021-02-11
期刊：
Nature communications
影响因子：
16.6
作者：
Lapenta F;Aupič J;Vezzoli M;Strmšek Ž;Da Vela S;Svergun DI;Carazo JM;Melero R;Jerala R
通讯作者：
Jerala R

LRET-derived HADDOCK structural models describe the conformational heterogeneity required for DNA cleavage by the Mre11-Rad50 DNA damage repair complex.