CSF-1 Gene Expression in Osteoclast Biology

破骨细胞生物学中的 CSF-1 基因表达

• 批准号: 8741919
• 负责人: SHERRY L ABBOUD-WERNER
• 金额: $ 30.65万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-30 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8741919
• 关键词: Age; Aging; Albers-Schonberg disease; Apoptosis; Biology; Bone Diseases; Bone remodeling; Breeding; Cell surface; Connexin 43; Connexins; Data; Defect; Dominant-Negative Mutation; Enzyme-Linked Immunosorbent Assay; Fracture; Gap Junctions; Gene Expression; Gene Expression Profile; Genes; Genetic; Goals; Health; Histology; Homeostasis; In Situ Hybridization; Knock-out; Lead; Longevity; Macrophage Colony-Stimulating Factor; Mechanics; Mitochondria; Modeling; Morbidity - disease rate; Mus; NADPH Oxidase; Osteoblasts; Osteoclasts; Osteocytes; Osteogenesis; Oxidases; Oxidation-Reduction; Oxidative Stress; Pathway interactions; Pattern; Phenotype; Protein Isoforms; Relative (related person); Reverse Transcriptase Polymerase Chain Reaction; Role; Signal Transduction; Small Interfering RNA; TNFSF11 gene; Testing; Therapeutic; Transgenic Organisms; Work; activity marker; age related; bone; bone loss; bone mass; bone strength; bone turnover; human FRAP1 protein; improved; insight; interest; laser capture microdissection; new therapeutic target; novel; novel therapeutics; overexpression; oxidant stress; prevent; public health relevance; receptor; skeletal; therapeutic target

DESCRIPTION (provided by applicant): Bone loss and fracture are leading causes of morbidity with aging. The mechanisms of age-related bone disease have not been defined. Our studies suggest that CSF-1 deficiency and increased Nox4 oxidase expression in osteocytes are key determinants of oxidant stress that impact osteocyte survival/function that is essential for bone remodeling. The long-term goal of this proposal is to delineate mechanistic pathways by which CSF-1/oxidative stress regulate osteocyte homeostasis and identify therapeutic targets to prevent bone loss with age. CSF-1 and CSF-1R are expressed by osteocytes. The mechanisms by which CSF-1 or its isoforms, soluble (s) and cell-surface (cs) CSF-1, regulate osteocyte survival/function have not been explored. Oxidative stress contributes to osteocyte demise and bone loss with aging. Our findings indicate that, with aging, CSF-1 expression declines in osteocytes. We generated mice with global CSF-1 deficiency (CSF-1KO) that show osteopetrosis with increased fractures and osteocyte defects including apoptosis, associated with increased NADPH oxidase Nox4 expression/activity and reduced Cx43 expression. CSF-1 decreases NADPH oxidase activity in cultured osteocytes and CSF-1KO bone osteocytes show elevated Nox4 and activation of the mTOR pathway compared to WT osteocytes, suggesting that CSF-1 protects from oxidant stress. DMP1Cre-CSF-1cKO mice with conditional knockout (cKO) of CSF-1 in osteocytes/late osteoblasts also show increased Nox4, osteocyte defects, reduced osteoclasts and bone formation, predisposing to bone loss and fracture with age. We hypothesize that: a) osteocyte cKO of CSF-1 increases Nox4 and oxidative stress, impairs osteocytes and accelerates bone defects with age, b) deletion of Nox4 in CSF-1cKO osteocytes decreases oxidative stress, restores osteocyte function/bone remodeling with age, c) expression of sCSF-1 in CSF-1cKO osteocytes promotes osteocyte survival and proper bone remodeling to a greater extent than csCSF-1 during aging. We will test these hypotheses in the following specific aims: 1) Determine the effect of CSF-1cKO in osteocytes on bone phenotype and redox state during aging. WT and CSF-1cKO mice will be examined for bone phenotype and osteocytes will be assessed for apoptosis, Nox4 and gene expression profile; 2) Determine the role of Nox4 in osteocytes of CSF-1cKO mice and mechanisms by which CSF-1 regulates osteocyte survival. To dissect the interplay between CSF-1 and Nox4, mice with cKO of CSF-1 and Nox4 in osteocytes will be generated and signaling mechanisms by which CSF-1 regulates osteocyte survival will be analyzed in cultured osteocytes; 3) Determine the ability of CSF-1 isoforms to rescue bone defects in CSF-1cKO mice. This will be accomplished using a transgenic approach to target sCSF-1 or csCSF-1 in osteocytes of CSF-1cKO mice. These studies will provide new mechanistic insights by which CSF-1 controls osteocyte survival/function and may lead to novel therapeutic strategies for improving osteocyte viability crucial for bone strength and longevity.

描述（由申请人提供）：骨丢失和骨折是随年龄增长而发病的主要原因。年龄相关性骨病的机制尚未确定。我们的研究表明，CSF-1缺乏和骨细胞中Nox 4氧化酶表达增加是氧化应激的关键决定因素，其影响骨细胞存活/功能，这对骨重建至关重要。该提案的长期目标是描绘CSF-1/氧化应激调节骨细胞稳态的机制途径，并确定治疗靶点以防止随年龄的骨丢失。CSF-1和CSF-1 R由骨细胞表达。CSF-1或其同种型，可溶性和细胞表面CSF-1，调节骨细胞存活/功能的机制尚未研究。随着年龄的增长，氧化应激导致骨细胞死亡和骨丢失。我们的研究结果表明，随着年龄的增长，CSF-1的表达在骨细胞下降。我们产生了具有全局CSF-1缺陷（CSF-1 KO）的小鼠，其显示骨硬化症，伴有骨折和骨细胞缺陷（包括凋亡）增加，与NADPH氧化酶Nox 4表达/活性增加和Cx43表达减少相关。CSF-1降低了培养的骨细胞中的NADPH氧化酶活性，并且与WT骨细胞相比，CSF-1 KO骨细胞显示出升高的Nox 4和mTOR途径的活化，表明CSF-1保护免受氧化应激。在骨细胞/晚期成骨细胞中条件性敲除（cKO）CSF-1的DMP 1Cre-CSF-1cKO小鼠也显示出增加的Nox 4、骨细胞缺陷、减少的破骨细胞和骨形成，随着年龄的增长易发生骨丢失和骨折。我们假设：a）CSF-1的骨细胞cKO增加Nox 4和氧化应激，损害骨细胞并加速随年龄的骨缺损，B）CSF-1 cKO骨细胞中Nox 4的缺失降低氧化应激，恢复随年龄的骨细胞功能/骨重建，c）在老化期间，sCSF-1在CSF-1 cKO骨细胞中的表达比csCSF-1更大程度地促进骨细胞存活和适当的骨重建。我们将在以下具体目标中检验这些假设：1）确定骨细胞中CSF-1cKO对衰老过程中骨表型和氧化还原状态的影响。将检查WT和CSF-1cKO小鼠的骨表型，并评估骨细胞的凋亡、Nox 4和基因表达谱; 2）确定Nox 4在CSF-1cKO小鼠的骨细胞中的作用以及CSF-1调节骨细胞存活的机制。为了剖析CSF-1和Nox 4之间的相互作用，将产生在骨细胞中具有CSF-1和Nox 4的cKO的小鼠，并将在培养的骨细胞中分析CSF-1调节骨细胞存活的信号传导机制; 3）确定CSF-1同种型挽救CSF-1cKO小鼠中的骨缺损的能力。这将使用靶向CSF-1cKO小鼠骨细胞中sCSF-1或csCSF-1的转基因方法来实现。这些研究将提供CSF-1控制骨细胞存活/功能的新机制见解，并可能导致改善骨细胞活力的新治疗策略，这对骨强度和寿命至关重要。