Mapping brainstem control of urine storage and voiding in conscious mice

绘制清醒小鼠尿液储存和排尿的脑干控制图

• 批准号: 8772700
• 负责人: Mark L. Zeidel
• 金额: $ 35.78万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8772700
• 关键词: Age; Animals; Area; Benign; Biological Neural Networks; Bladder; Bladder Diseases; Boston; Brain; Brain Stem; Central Nervous System Diseases; Conscious; Designer Drugs; Development; Diabetes Mellitus; Disease; Elderly; Electrodes; Functional Imaging; Future; Genetic; Goals; Health; Human; Individual; Institution; Intervention; Knowledge; Lead; Lesion; Light; Link; Lower urinary tract; Maps; Methods; Modality; Modeling; Mus; Neurons; Neurotransmitters; Pathogenesis; Patients; Pharmaceutical Preparations; Physiology; Pontine structure; Population; Rabies virus; Research; Research Personnel; Resource Development; Role; Running; Site; Spinal Cord; Staging; Stress; Symptoms; Synapses; Testing; Therapeutic; Urethra; Urination; Urine; Urologist; Urology; base; cost; effective therapy; graduate student; human subject; improved; insight; lower urinary tract symptoms; magnetic field; mind control; mouse model; nervous system disorder; neural circuit; neuroregulation; new technology; novel; novel diagnostics; novel therapeutics; optogenetics; patient population; programs; public health relevance; receptor; relating to nervous system; response; symposium; tool

DESCRIPTION (provided by applicant): Lower urinary tract symptoms and related disturbances represent a major health problem, for which there are few reliable and effective treatments. We lack therapies because we do not understand the underlying causes, including the role of disturbances in the brain control of urine storage and voiding. The overall goal of the PI is to develop the mouse as a model of LUTS, exploiting mouse genetics (the PI's current P20) and new technologies for mapping neural circuits in mice (the current proposal). The Center is a Resource Development Project, which seeks to use state of the art neural mapping methods, including optogenetics, Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), and rabies virus synapse tracing, to determine the functional roles of specific pontine micturition center (PMC) neuron subpopulations in urine storage and voiding. The impact of activating or silencing PMC neuron subpopulations on spontaneous voiding and conscious cystometrograms will be determined. Detailed knowledge of the function of PMC neuron subpopulations, along with an understanding of which neurons synapse on them will set the stage in future years for investigators in our group and others to map out in detail the entire neural network controlling urine storage and voiding. The enrichment program will focus on bringing to the field of benign urology world leading neurobiologists, and developing future investigators in CNS control of urine storage and voiding (including the graduate student supported in the proposal. In addition, we will run symposia to help identify urologists at our own institution and others in Boston who can help us to develop human subjects studies which will allow us to extend to patients the insights which we are developing in mice.

描述（由申请人提供）：下尿路症状和相关紊乱是一个主要的健康问题，目前很少有可靠有效的治疗方法。我们缺乏治疗方法，因为我们不了解潜在的原因，包括大脑控制尿液储存和排尿的干扰。总体目标