Transcriptional Genomics Core

这位PPG建议使用基于深度测序的方法来定义转录机制， 调节炎症和胰岛素抵抗。这三个项目的一个基本方面将是定义 调节蛋白功能的获得和丧失对巨噬细胞和 使用大规模平行RNA测序技术的脂肪细胞基因表达。这三个项目也将 利用ChlP测序技术确定野生型和突变型PPARγ和 NCoR/SMRT共抑制物复合体的组成。最后，项目2和3将使用最近开发的 三维基因组相互作用分析(3D-DSL)研究核与核之间的关系 建筑和基因表达。PPG深度测序核心将提供两项补充服务 为推进这些工作；为准备和测序所需的特定文库提供技术支持 每项化验和生物信息学/计算支持，以实现有效、有效的实验设计和 结果数据的利用率。

This PPG proposes to use deep sequencing-based approaches to define transcriptional mechanisms that regulate inflammation and insulin resistance. An essential aspect of all three projects will be to define the consequences of gain and loss of function of regulatory proteins on global programs of macrophage and adipocyte gene expression using massively parallel RNA sequencing technologies. All three projects will also employ ChlP-Sequencing technologies to define the cistromes of wild type and mutant PPARγ and components of NCoR/SMRT co-repressor complexes. Finally, Projects 2 and 3 will use recently developed assays of three-dimensional genomic interactions (3D-DSL) to interrogate the relationship between nuclear architecture and gene expression. The PPG Deep Sequencing Core will provide two complementary services to advance these efforts; technical support for preparation and sequencing of the specific libraries required for each assay and bioinformatics/computational support to enable effective effective experimental design and utilization of the resulting data.