(Basic Shared Resources) Comparative Pathology

（基本共享资源）比较病理学

• 批准号: 8633119
• 负责人: Douglas Yee
• 金额: $ 11.22万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2019-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8633119
• 关键词: Advisory Committees; Agreement; Alleles; Animals; Apoptotic; Area; Autopsy; Blood; Brain Neoplasms; Bromodeoxyuridine; CD3 Antigens; Cancer Center; Cancer Center Support Grant; Candidate Disease Gene; Canis familiaris; Carcinogens; Cells; Chemoprevention; Childhood; Clinical Chemistry; Clinical Research; Clinical Trials; Colorectal Cancer; Comparative Pathology; Computer software; Consultations; Data; Detection; Devices; Doctor of Philosophy; Drug Delivery Systems; Dyes; Ensure; Equipment; Evaluation; Formalin; Freezing; Funding; Genes; Genetic; Genetic Screening; Glucagon; Grant; Health; Hematology; Hematoxylin and Eosin Staining Method; Histology; Human; Human Resources; Image; Image Analysis; Immunofluorescence Immunologic; Immunohistochemistry; Immunology; Immunotherapy; Insulin; Journals; Label; Laboratories; Laboratory Animals; Laboratory Research; Leadership; Licensing; Magnetic Resonance Imaging; Malignant Neoplasms; Manuscripts; Measurement; Microscopic; Minnesota; Mission; Monitor; Mus; N' -nitrosonornicotine; Oral; Oral mucous membrane structure; PTPRC gene; Paraffin; Pathologist; Pathology; Patients; Periderm; Phenotype; Predisposition; Preparation; Process; Proliferating; Proteins; Protocols documentation; Publications; Publishing; Rattus; Recruitment Activity; Relative (related person); Reporting; Research; Research Design; Research Personnel; Research Project Grants; Research Support; Resolution; Resource Sharing; Resources; Scanning; Services; Sleeping Beauty; Slide; Smokeless Tobacco; Specimen; Staining method; Stains; TdT-Mediated dUTP Nick End Labeling Assay; Techniques; Time; Tissue Procurements; Tissues; Transgenic Animals; Tyrosine Kinase Inhibitor; Universities; Vaccines; Veterinary Pathology; animal tissue; anticancer research; base; bone; carcinogenesis; carcinogenicity; caspase-3; comparative; cyanine dye 5; digital; digital imaging; experience; gene therapy; impression; improved; in vivo; indexing; interest; meetings; member; morphometry; novel strategies; programs; receptor; soft tissue; tissue fixing; tumor

The mission ofthe Comparative Pathology Shared Resource (CPSR) is to provide full-service pathology support for Masonic Cancer Center (MCC) members who use animal subjects in their laboratory and clinical research. CPSR is a critical resource for projects utilizing animal tissues and is essential for publications involving pathological changes in tissues. M. Gerard O'Sullivan, MVB, PhD, took over the leadership of CSPR in 2009; Dr. O'Sullivan has more than 20 years of experience in comparative and experimental veterinary pathology. Also in 2009, CPSR recruited a very experienced histotechnologist, Paula Overn. Three histotechnicians prepare appropriately processed fixed or frozen tissues for histology and immunohistochemistry. Particular strengths ofthe Shared Resource are in doing non-routine challenging or tedious histological preparations that require special handling, such as sectioning and performing immunohistochemistry on bone. Board-certified veterinary pathologists provide expert gross and histopathologic analyses, generate high-resolution digital images of specimens, and provide intellectual input on study design and manuscript preparation for research projects and grant applications. The CPSR also has considerable experience in developing new protocols and techniques and has developed approximately 60 new immunohistochemistry markers since 2008. In addition, the CPSR has developed double-labeling techniques to simultaneously detect several combinations of proteins. Requests for quantification of marker expression and histomorphometric evaluations have increased, and CSPR has gained access to equipment and software to accommodate these requests. Weekly staff meetings and the oversight of an advisory committee composed of CPSR users ensure that the resource is able to meet the needs of MCC members for veterinary pathology.

比较病理学共享资源（CPSR）的使命是为在实验室和临床研究中使用动物受试者的共济会癌症中心（MCC）成员提供全方位的病理学支持。CPSR是利用动物组织项目的重要资源，对于涉及组织病理变化的出版物至关重要。M. Gerard O'Sullivan， MVB, PhD，于2009年接管CSPR的领导；奥沙利文博士在比较和实验兽医病理学方面拥有20多年的经验。同样在2009年，CPSR聘请了一位非常有经验的组织技术专家Paula Overn。三名组织技术人员准备适当处理的固定或冷冻组织用于组织学和免疫组织化学。共享资源的特殊优势在于进行非常规的具有挑战性或繁琐的组织学准备，这些准备需要特殊处理，例如骨切片和免疫组织化学。委员会认证的兽医病理学家提供专家的大体和组织病理学分析，生成标本的高分辨率数字图像，并为研究项目和拨款申请提供研究设计和手稿准备方面的知识输入。CPSR在开发新方案和技术方面也有相当的经验，自2008年以来已经开发了大约60种新的免疫组织化学标记物。此外，CPSR还开发了双标记技术，可以同时检测多种蛋白质组合。量化标记表达和组织形态计量评估的需求增加了，CSPR已经获得了设备和软件来满足这些需求。每周的工作人员会议和由CPSR用户组成的咨询委员会的监督确保资源能够满足MCC成员对兽医病理学的需求。