(Basic Shared Resources) Comparative Pathology

比较病理学共享资源(CPSR)的使命是为共济会癌症中心(MCC)在实验室和临床研究中使用动物对象的成员提供全方位的病理学支持。CPSR是利用动物组织的项目的关键资源，对于涉及组织病理变化的出版物也是必不可少的。M.Gerard O‘Sullivan，MVB，博士，于2009年接任CSPR的领导；O’Sullivan博士在比较和实验兽医病理学方面拥有20多年的经验。同样在2009年，CPSR聘请了一位非常有经验的组织技术学家Paula Overn。三名组织技术人员准备经过适当处理的固定或冷冻组织，用于组织学和免疫组织化学。共享资源的特殊优势在于进行需要特殊处理的非常规、具有挑战性或繁琐的组织学准备，例如对骨骼进行切片和执行免疫组织化学。委员会认证的兽医病理学家提供专家大体和组织病理学分析，生成高分辨率的标本数字图像，并为研究项目和拨款申请提供研究设计和手稿准备方面的智力投入。CPSR在开发新的方案和技术方面也有相当的经验，自2008年以来已经开发了大约60个新的免疫组织化学标记物。此外，CPSR还开发了双标记技术，以同时检测几种蛋白质组合。对标记物表达和组织形态计量学评估的量化要求有所增加，CSPR已获得满足这些要求的设备和软件。每周的工作人员会议和由CPSR用户组成的咨询委员会的监督确保资源能够满足MCC成员对兽医病理学的需求。

The mission ofthe Comparative Pathology Shared Resource (CPSR) is to provide full-service pathology support for Masonic Cancer Center (MCC) members who use animal subjects in their laboratory and clinical research. CPSR is a critical resource for projects utilizing animal tissues and is essential for publications involving pathological changes in tissues. M. Gerard O'Sullivan, MVB, PhD, took over the leadership of CSPR in 2009; Dr. O'Sullivan has more than 20 years of experience in comparative and experimental veterinary pathology. Also in 2009, CPSR recruited a very experienced histotechnologist, Paula Overn. Three histotechnicians prepare appropriately processed fixed or frozen tissues for histology and immunohistochemistry. Particular strengths ofthe Shared Resource are in doing non-routine challenging or tedious histological preparations that require special handling, such as sectioning and performing immunohistochemistry on bone. Board-certified veterinary pathologists provide expert gross and histopathologic analyses, generate high-resolution digital images of specimens, and provide intellectual input on study design and manuscript preparation for research projects and grant applications. The CPSR also has considerable experience in developing new protocols and techniques and has developed approximately 60 new immunohistochemistry markers since 2008. In addition, the CPSR has developed double-labeling techniques to simultaneously detect several combinations of proteins. Requests for quantification of marker expression and histomorphometric evaluations have increased, and CSPR has gained access to equipment and software to accommodate these requests. Weekly staff meetings and the oversight of an advisory committee composed of CPSR users ensure that the resource is able to meet the needs of MCC members for veterinary pathology.