Tissue Banks

组织库

• 批准号: 8727981
• 负责人: Christine A Iacobuzio-Donahue
• 金额: $ 11.75万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8727981
• 关键词: Address; Area; Autopsy; Basic Science; Biological; Blood; Blood specimen; Cancer Etiology; Cell Line; Cessation of life; Clinical; Collaborations; Collection; Colon; Colorectal Adenoma; Colorectal Cancer; Colorectal Neoplasms; Consultations; Data; Diagnosis; Disasters; Disease; Excision; Family; Formalin; Fostering; Freezing; Harvest; Histologic; Histopathology; Human; Individual; Institution; Large Intestine Carcinoma; Liquid substance; Malignant Epithelial Cell; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Metastatic Neoplasm to the Liver; National Cancer Institute; National Research Council; Neoplasms; Nude Mice; Online Systems; Outcome; Pancreas; Pancreatic carcinoma; Paraffin Embedding; Participant; Pathologic; Pathologist; Patient Care; Patients; Practice Guidelines; Process; Publications; Recommendation; Recording of previous events; Recovery; Research Personnel; Resource Sharing; Resources; Risk; Sampling; Site; Specialist; Specimen; Staging; Supervision; System; Tissue Banking; Tissue Banks; Tissue Microarray; Tissues; Translational Research; United States; World Health Organization; Xenograft procedure; biobank; clinical material; encryption; human tissue; neoplastic; pancreatic juice; pancreatic neoplasm; tissue preparation; tumor; tumor xenograft; web based interface

The purpose of this shared resource is to provide human tissues, biological fluids, and expert pathologic interpretation for investigators in all of the projects under the direction of expert pathologists. The Histopathology and Tissue/Biologic Fluids Resource has been in existence since 1986 and expanded under the support of the GI SPORE. As of this year, the Resource includes 1698 banked colorectal carcinoma resection specimens, 354 colorectal adenoma samples, 325 hepatic metastases of colorectal carcinoma, 1013 pancreatic carcinoma specimens, 588 xenografts of colorectal carcinoma, 101 pancreatic carcinoma xenografts, 5298 blood specimens from individuals at risk for colorectal carcinoma, and 867 blood specimens from patients with pancreatic carcinoma. Formalin-fixed paraffin-embedded tissues, frozen materials and clinical and outcome data are available for the vast majority of resection specimens. Most recently, this resource has expanded its collections to include high quality snap frozen and formalin fixed samples of end stage pancreatic or colorectal cancer from 120 rapid autopsy participants. 133 tissue microarrays of colorectal and pancreatic neoplasms and diseases have been created. Eight fully characterized pancreatic carcinoma cell lines have been prepared from human tumors and shared with the ATCC. The biospecimens are harvested and banked in accordance with the National Cancer Institute's Best Practice Guidelines for Biorepositories. Distribution of these specimens to SPORE investigators has resulted in over 100 publications. This Core procures additional xenografts, fresh frozen colorectal and pancreatic neoplasms and blood and pancreatic juice samples, and provides expert pathologic consultation to investigators. Specimens are collected under the supervision of pathologists with expertise in colorectal and pancreatic neoplasia in close collaboration with clinical specialists in these areas and in similarly close collaboration with basic research investigators to maximize translational impact of the projects. Clinical and family histories are entered into a password protected web-based tracking system for our pancreatic cases. This Web-based interface follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins GI Cancer SPORE. Biosamples have been shared with investigators at over 50 other institutions. Collaboration with investigators at other centers fosters the accrual of rare tumor types.

该共享资源的目的是在专家病理学家的指导下为所有项目的研究人员提供人体组织，生物液体和专家病理解释。组织学和组织/生物液体资源自1986年以来一直存在，并在GI孢子的支持下扩大。截至今年，该资源包括1698个储存的结直肠癌切除标本，354个结直肠腺瘤标本，325个结直肠癌肝转移，1013个胰腺癌标本，588个结直肠癌异种移植物，101个胰腺癌异种移植物，5298个来自结直肠癌风险个体的血液标本，胰腺癌患者血液标本867例。福尔马林固定石蜡包埋组织、冷冻材料以及临床和结局数据可用于绝大多数切除标本。最近，该资源已扩大其收藏，包括来自120名快速尸检参与者的终末期胰腺癌或结直肠癌的高质量速冻和福尔马林固定样本。已经创建了133个结直肠和胰腺肿瘤和疾病的组织微阵列。已经从人肿瘤中制备了八种完全表征的胰腺癌细胞系，并与ATCC共享。根据国家癌症研究所的生物储存库最佳实践指南收获生物标本并储存。将这些标本分发给SPORE研究人员后，发表了100多篇论文。该中心采购额外的异种移植物、新鲜冷冻的结直肠和胰腺肿瘤以及血液和胰液样本，并为研究者提供专家病理咨询。在具有结直肠和胰腺肿瘤专业知识的病理学家的监督下收集标本，并与这些领域的临床专家密切合作，并与基础研究调查人员密切合作，以最大限度地发挥项目的转化影响。临床和家族史被输入到一个密码保护的基于网络的跟踪系统中，用于我们的胰腺病例。这个基于Web的界面遵循国家研究理事会的建议，包括用户身份验证、加密、审计跟踪和灾难恢复。已建立一种机制，用于优先向约翰霍普金斯GI癌症孢子内外的研究者分配所需资源。已与50多个其他机构的调查人员分享了生物样本。与其他中心的研究者合作促进了罕见肿瘤类型的增加。