Tissue Banks

这一共享资源的目的是在专家病理学家的指导下，为所有项目的研究人员提供人体组织、生物液体和专家病理解释。组织病理学和组织/生物流体资源自1986年以来一直存在，并在GI孢子的支持下扩大。截至今年，该资源包括1698份结直肠癌切除标本、354份结直肠癌腺瘤标本、325份结直肠癌肝转移标本、1013份胰腺癌标本、588份结直肠癌异种移植标本、101份胰腺癌异种移植标本、5298份结直肠癌高危个体血液标本和867份胰腺癌患者血液标本。福尔马林固定石蜡包埋组织，冷冻材料和临床和结局数据可用于绝大多数切除标本。最近，该资源扩大了其收藏范围，包括来自120名快速尸检参与者的高质量快速冷冻和福尔马林固定的终末期胰腺癌或结直肠癌样本。133个结直肠和胰腺肿瘤和疾病的组织微阵列已经创建。从人类肿瘤中制备了8个完全表征的胰腺癌细胞系，并与ATCC共享。根据国家癌症研究所的生物储存最佳实践指南，收集和储存生物标本。将这些标本分发给孢子调查人员已导致100多篇出版物。该中心获取额外的异种移植物、新鲜冷冻结直肠和胰腺肿瘤以及血液和胰腺液样本，并为研究人员提供专家病理咨询。标本的收集在具有结肠直肠和胰腺肿瘤专业知识的病理学家的监督下，与这些领域的临床专家密切合作，并与基础研究人员密切合作，以最大限度地提高项目的转化影响。临床和家族史被输入到一个密码保护的基于网络的胰腺病例跟踪系统中。这个基于web的界面遵循国家研究委员会的建议，包括用户身份验证、加密、审计跟踪和灾难恢复。一种机制已经到位，以优先分配所要求的资源给约翰霍普金斯大学胃肠道癌症孢子内部和外部的研究人员。生物样本已与其他50多个机构的调查人员共享。与其他中心的研究人员的合作促进了罕见肿瘤类型的积累。

The purpose of this shared resource is to provide human tissues, biological fluids, and expert pathologic interpretation for investigators in all of the projects under the direction of expert pathologists. The Histopathology and Tissue/Biologic Fluids Resource has been in existence since 1986 and expanded under the support of the GI SPORE. As of this year, the Resource includes 1698 banked colorectal carcinoma resection specimens, 354 colorectal adenoma samples, 325 hepatic metastases of colorectal carcinoma, 1013 pancreatic carcinoma specimens, 588 xenografts of colorectal carcinoma, 101 pancreatic carcinoma xenografts, 5298 blood specimens from individuals at risk for colorectal carcinoma, and 867 blood specimens from patients with pancreatic carcinoma. Formalin-fixed paraffin-embedded tissues, frozen materials and clinical and outcome data are available for the vast majority of resection specimens. Most recently, this resource has expanded its collections to include high quality snap frozen and formalin fixed samples of end stage pancreatic or colorectal cancer from 120 rapid autopsy participants. 133 tissue microarrays of colorectal and pancreatic neoplasms and diseases have been created. Eight fully characterized pancreatic carcinoma cell lines have been prepared from human tumors and shared with the ATCC. The biospecimens are harvested and banked in accordance with the National Cancer Institute's Best Practice Guidelines for Biorepositories. Distribution of these specimens to SPORE investigators has resulted in over 100 publications. This Core procures additional xenografts, fresh frozen colorectal and pancreatic neoplasms and blood and pancreatic juice samples, and provides expert pathologic consultation to investigators. Specimens are collected under the supervision of pathologists with expertise in colorectal and pancreatic neoplasia in close collaboration with clinical specialists in these areas and in similarly close collaboration with basic research investigators to maximize translational impact of the projects. Clinical and family histories are entered into a password protected web-based tracking system for our pancreatic cases. This Web-based interface follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins GI Cancer SPORE. Biosamples have been shared with investigators at over 50 other institutions. Collaboration with investigators at other centers fosters the accrual of rare tumor types.