Automated High-throughput Platform for Tissue Homogenization and RNA Extraction

总结 研究用核酸样品制备的整体市场规模和食品的四个应用市场 2012年，检测、化妆品、兽医和分子诊断总计57亿美元， 2016年10%的适度复合年增长率预计为97亿美元12-5。从组织中提取核酸， 然而RNA的样品制备和分离仍然严重依赖于手工步骤。 提供用于组织制备和RNA分离的快速、自动化系统是未满足的需求。我们有一个 独特的细胞和组织裂解系统，微匀浆器（mH），这是一种低成本，一次性和快速 有效单位。它由一个一次性电机和连接到移液器吸头的叶片组成。这 便于容易地适应于任何自动化和多路复用的机器人液体处理系统。我们 准备一次多路复用八个样品，以微量滴定形式进行均质化，从而使 自动化随后的RNA提取方案，一次96个样品。100磅的提取系统 单分散的珠粒填充到小柱中有助于提取方案， 离心机。这使得快速自动化的提取方案，在微量滴定格式，多达96个样品， 时间

Summary The overall market size for nucleic acid sample preparation for research and the four applied markets of food testing, cosmetics, veterinary medicine, and molecular diagnostics total $5.7 billion in 2012 and with a moderate CAGR of 10 % is projected to $9.7 billion in 2016 12-5. Extraction of nucleic acids from tissue has become a crowded field yet sample preparation and isolation of RNA still relies heavily on manual steps. Providing a rapid, automated system for tissue preparation and RNA isolation is an unmet need. We have a unique cell and tissue lysing system, the microHomogenizer (mH), which is a low cost, disposable and rapidly effective homogenizing unit. It consists of a disposable motor and vane that attaches to a pipette tip. This facilitates ready adaptation to any robotic liquid handing system for automation and multiplexing. We are poised to multiplex eight samples at a time for homogenization in a microtiter format, thereby enabling automation of the subsequent RNA extraction protocol for 96 samples at a time. The extraction system of 100¿ mono-dispersed beads packed into small columns facilitates extraction protocols that transcend the need of a centrifuge. This enables rapid automation of the extraction protocols in a microtiter format, up 96 samples at time.