Automated High-throughput Platform for Tissue Homogenization and RNA Extraction

摘要 研究用核酸样品制备的总体市场规模和食品的四个应用市场 2012年，检测、化妆品、兽医和分子诊断行业的总销售额为57亿美元， 预计2016年12-5年的适度复合年增长率为10%，达到97亿美元。从组织中提取核酸具有 然而，RNA的样品制备和分离仍然严重依赖于人工步骤。 提供一种快速、自动化的组织准备和RNA分离系统是一个尚未得到满足的需求。我们有一个 独特的细胞和组织裂解系统，微均质器(MH)，低成本，一次性和快速 有效的均化单元。它由一个一次性马达和连接到吸管尖端的叶片组成。这 便于随时适应任何自动化和多路传输的机器人液体处理系统。我们是 准备一次多路传输八个样本，以便以微滴定格式均化，从而实现 一次对96个样本的后续RNA提取协议的自动化。100？的提取系统 填充在小柱子中的单分散珠子有助于提取协议，而不需要 离心机。这使得微量滴定格式的提取协议能够快速自动化，最多96个样品 时间到了。

Summary The overall market size for nucleic acid sample preparation for research and the four applied markets of food testing, cosmetics, veterinary medicine, and molecular diagnostics total $5.7 billion in 2012 and with a moderate CAGR of 10 % is projected to $9.7 billion in 2016 12-5. Extraction of nucleic acids from tissue has become a crowded field yet sample preparation and isolation of RNA still relies heavily on manual steps. Providing a rapid, automated system for tissue preparation and RNA isolation is an unmet need. We have a unique cell and tissue lysing system, the microHomogenizer (mH), which is a low cost, disposable and rapidly effective homogenizing unit. It consists of a disposable motor and vane that attaches to a pipette tip. This facilitates ready adaptation to any robotic liquid handing system for automation and multiplexing. We are poised to multiplex eight samples at a time for homogenization in a microtiter format, thereby enabling automation of the subsequent RNA extraction protocol for 96 samples at a time. The extraction system of 100¿ mono-dispersed beads packed into small columns facilitates extraction protocols that transcend the need of a centrifuge. This enables rapid automation of the extraction protocols in a microtiter format, up 96 samples at time.