Excitation-Contraction Coupling in Normal and Dystrophic Mammalian Muscle

描述（由申请人提供）：本提案的总体目标是深入了解哺乳动物骨骼肌中肌营养不良蛋白糖蛋白复合物（DGC）的改变与兴奋-收缩耦合（ECC）过程损伤之间的机制联系。这种功能表征将在各种人类肌肉萎缩症动物模型的肌纤维中实现。我们发现，在成年mdx小鼠和表型肌跨（SSPN）过表达小鼠（SSPN- tg）的两种模型中，动作电位（APs）（或电压钳脉冲）在肌纤维中引起的Ca2+释放明显小于野生型纤维。我们假设DGC的破坏破坏了横管系统（TTS）和肌浆网（SR）的结构和功能支持，从而减弱了ECC过程。我们将首先研究mdx小鼠Ca2+释放损伤的机制（Aim 1）， mdx是杜氏肌营养不良症（DMD）最常用的动物模型，DGC中缺乏肌营养不良蛋白。然而，由于mdx小鼠的表型改变是相对良性的，可能是由于DGC中的utrophin替代，因此我们还将在双敲除mdx/utrophin （mdx/utr-/-）小鼠中进行实验，其表型与DMD患者更相似（Aim 2）。为了进一步表征DGC完整性与全功能ECC之间的联系，我们将利用我们通过体内电穿孔表达DGC蛋白的能力，并使用具有其他遗传条件改变DGC（例如SSPN-Tg和ut - tet）的转基因动物模型。该提案的最后一个目标是使用双光子激光扫描显微镜（TPLSM）研究代表性DGC蛋白组分的亚细胞分布，以评估它们是否仅与肌膜相关，或者它们是否与z线和TTS相关具有更普遍的分布。这一特征将有助于我们了解DGC在ECC和肌上皮完整性方面的功能（目的3）。这些研究将通过电生理学和最先进的光学方法进行，如F"ster共振能量转移（FRET）和全内反射荧光显微镜（TIRFM），还将评估DGC和ECC蛋白在表面和TTS膜的内部和外部小叶上的纳米级定位。

DESCRIPTION (provided by applicant): The overall goal of this proposal is to obtain an in-depth understanding of the mechanistic links between alterations of the dystrophin glycoprotein complex (DGC) and impairment of the excitation-contraction coupling (ECC) process in mammalian skeletal muscle. This functional characterization will be achieved in muscle fibers from various animal models of human muscular dystrophies. We have found that Ca2+ release evoked by action potentials (APs) (or voltage-clamp pulses) in muscle fibers from two of such models, the adult mdx mouse and the phenotypic sarcospan (SSPN) overexpressing mouse (SSPN-Tg), is significantly smaller than in wild type fibers. We hypothesize that disruption of the DGC undermines the structural and functional support for the transverse tubular system (TTS) and the sarcoplasmic reticulum (SR), thus attenuating the ECC process. We will first investigate the mechanisms responsible for the impairment of Ca2+ release in mdx mice (Aim 1), the most prevalently used animal model for Duchenne Muscular Dystrophy (DMD), which lacks dystrophin in the DGC. However, since the phenotypic alterations in mdx mice are relatively benign, possibly due to utrophin substitution in the DGC, experiments will be also carried out in double knockout mdx/utrophin (mdx/utr-/-) mice that display a phenotype more comparable to that in DMD patients (Aim 2). To further characterize the link between the DGC integrity and a fully functional ECC, we will take advantage of our ability to express DGC proteins by in vivo electroporation and use transgenic animal models with other genetic conditions altering the DGC (e.g. SSPN-Tg, and Utr-TET). The last goal of the proposal is to investigate, using 2-photon laser scanning microscopy (TPLSM) the subcellular distribution of representative DGC protein components in order to assess if they are associated exclusively with the sarcolemma or if they have a more ubiquitous distribution in association with the Z-line and the TTS. This characterization will help us understand the function of the DGC in terms of ECC and sarcolemmal integrity (Aim 3). These investigations will be carried out by using electrophysiological and state-of-the-art optical methods, such as F"ster resonance energy transfer (FRET) and total internal reflection fluorescence microscopy (TIRFM), to also assess the nanoscale localization of DGC and ECC proteins with respect to the internal and external leaflets of the surface and TTS membranes.