Core B - Skin Pathology Core

核心 B - 皮肤病理学核心

• 批准号: 8540336
• 负责人: Jag Bhawan
• 金额: $ 17.86万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8540336
• 关键词: Academic Medical Centers; Autoantibodies; Biological Markers; Biopsy; Bleomycin; Blood Vessels; Boston; Cell Count; Cessation of life; Clinical; Clinical Data; Clinical Trials; Collagen; Connective Tissue; Correlation Studies; Data Set; Databases; Dermatopathology; Diffuse Scleroderma; Disease; Disease Progression; Evaluation; Evolution; Fibrosis; Formalin; Frozen Sections; Funding; Histocytochemistry; Human; Image Analysis; Immune response; Immunohistochemistry; Immunologic Markers; Individual; Inflammation; Inflammatory; Keloid; Label; Laboratories; Leadership; Methods; Microdissection; Modeling; Mus; Myofibroblast; Normal tissue morphology; Organ; Pathogenesis; Pathologic; Pathologic Processes; Pathology; Pathway interactions; Patients; Pattern; Preparation; Process; Progressive Disease; Proteins; Protocols documentation; Psoriasis; Research; Research Personnel; Resources; Rheumatism; Sampling; Scleroderma; Services; Skin; Skin Tissue; Slide; Specificity; Specimen; Staining method; Stains; Systemic Scleroderma; Testing; Therapeutic; Therapeutic Effect; Time; Tissue Banking; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; United States National Institutes of Health; Vascular Diseases; drug discovery; illness length; new technology; novel; programs; protein expression; trait; wound

Skin samples from patients with systemic sclerosis (SSc) are not widely available to investigators studying pathogenesis. To derive the greatest value from these samples it is important that they are associated with clinical data, in particular the modified Rodnan skin score (MRSS), but also ideally other organ involvement The Boston University Medical Center Scleroderma Program under the leadership of Dr. Lafyatis and through NIH U01 funding has collected and is continuing to collect such well-characterized biopsies. In addition in many cases in patients with early diffuse SSc biopsies are being collected every 6 months, so that the evolution of the disease and the relationship to changes in protein staining in the skin tissues can be compared. Dr. Bhawan, Dermatopathologist and Director of the BUMC Dermatopathology Laboratory, will direct the Skin Pathology Core (Core B). The Core will process these and other biopsies provided by Core Center Investigators into 8-sample tissue blocks containing either 6 different SSc and 2 healthy control biopsies ("Discovery slides"), 6 temporally evolving skin changes in individual patients and 2 healthy control biopsies ("Disease Progression slides"), or 2 SSc and 6 other diseases including wound tissue, psoriasis, keloid and nephrogenic systemic fibrosis (Specificity slides). Murine skin will be collected from murine models of SSc: cGVHD, bleomycin, Tsk and other SSc models, as well as control mice for similar multi-tissue slide preparations. The Core will assist Investigators or stain these slides in the Core as the Investigator requests with proteins under study for new pathological processes implicated in the vasculopathy, fibrotic or immune response in SSc and murine SSc model skin. Core B will also evaluate histochemical or immunohistochemical staining intensity using quantitative (image analysis) or semi-quantitative methods. Sample staining will be correlated with MRSS and other clinical parameters, and past staining patterns in slides from the same block can also be compared. These slides will provide an unparalleled resource for discovery of pathogenic mechanisms in skin pathogenesis of SSc.

来自系统性硬化症（SSc）患者的皮肤样本并没有被广泛用于研究 发病机制为了从这些样本中获得最大价值，重要的是将它们与 临床数据，特别是改良Rodnan皮肤评分（MRSS），但理想情况下还包括其他器官受累 波士顿大学医学中心硬皮病项目在Lafyatis博士的领导下， 通过NIH U01的资助，已经收集并将继续收集这种特征良好的活检。在 此外，在许多早期弥漫性SSc患者中，每6个月收集一次活检， 这种疾病的发展以及与皮肤组织中蛋白质染色变化的关系， 比较了Bhawan博士，皮肤病理学家和BUMC皮肤病理学实验室主任，将 引导皮肤病理学核心（核心B）。核心将处理核心提供的这些和其他活检 中心研究者将8个样本组织块（包含6个不同SSc和2个健康对照） 活检（“Discovery载玻片”），6例个体患者和2例健康对照的时间演变皮肤变化 活检（“疾病进展载玻片”），或2例SSc和6例其他疾病，包括伤口组织，银屑病， 瘢痕疙瘩和肾源性系统性纤维化（特异性切片）。将从小鼠中采集小鼠皮肤 SSc模型：cGVHD、博莱霉素、Tsk等SSc模型，以及相似的对照小鼠 多组织载玻片制备。核心将协助研究者或将核心中的这些载玻片染色， 研究者要求研究中的蛋白质参与新的病理过程， SSc和鼠SSc模型皮肤中的血管病变、纤维化或免疫应答。核心B还将评估 组织化学或免疫组织化学染色强度使用定量（图像分析）或 半定量方法。样本染色将与MRSS和其他临床参数相关， 也可以比较来自同一块的载玻片中的过去染色模式。这些幻灯片将提供 发现SSc皮肤发病机制的无与伦比的资源。