Animal Core

动物核心将为所有四个项目提供主要的动物模型（条件Kras[G12D]小鼠模型）。杂合LSL-Kras[G12D/+]小鼠将与杂合Pdx-1-Cre小鼠杂交。后代将使用小鼠尾部DNA的PCR分析对突变Kras等位基因和Cre转基因的存在进行基因分型。突变小鼠{LSL-Kras[G12D/+]；Pdx-1-Cre)将被随机分配到对照饮食（AIN-76A）或高脂肪、高热量饮食（HFCD）。然后，每个实验组将被分配到个别项目特定的治疗和干预措施中。动物核心将进行与项目特定动物研究相关的所有方面。根据机构指南，将在3,6,9和12个月时牺牲小鼠或过早终止。在献祭时，包括整个胰腺、其他器官和血液在内的组织将被采集和加工。确切的后处理程序取决于个别项目的要求和需要。一般来说，胰腺的一半将被固定在福尔马林中并包埋在石蜡中（用于标准组织学和免疫组织化学），另一半将被冷冻在液氮中（用于RNA和蛋白质分析）。一小块胰腺将用于基因分型（以检测成功的重组事件）。动物核心包括病理亚核心，它将提供所有小鼠组织的组织病理学评估。动物中心为每个项目提供的服务包括：获得并保持机构批准，在后勤和统计能力计算方面设计动物研究（连同Pi项目和生物统计子核心），维持动物品系并建立繁殖对，对所有动物进行基因分型，将动物随机分配到实验组，准备和管理实验饮食或干预措施，日常监测和动物护理，动物安乐死，在献祭时收获组织和血液，处理组织，例如用福尔马林固定和石蜡包埋，切片（连同病理亚核心），组织切片的组织病理学评估（连同病理亚核心），细胞培养（panln和小鼠胰腺癌）的生成，原始数据的存储，组织和生物样本的中央银行，组织分配到各个项目，与项目负责人讨论结果，对项目调查员进行动物实验方面的培训（如果需要）。

The Animal Core will provide the main animal model (conditional Kras[G12D] mouse model) to all four projects. Heterozygous LSL-Kras[G12D/+] mice will be crossed with heterozygous Pdx-1-Cre mice. Offspring will be genotyped for the presence of the mutated Kras allele and Cre transgene using PCR analysis of mouse tail DNA. Mutant mice {LSL-Kras[G12D/+];Pdx-1-Cre) will be randomly allocated to either the control diet (AIN-76A) or the high fat, high calorie diet (HFCD). Each of the experimental groups will then be allocated to the individual project-specific treatments and interventions. The Animal Core will conduct all aspects related to the Project-specific animal studies. Cohorts of mice will be sacrificed at 3, 6, 9, and 12 months or prematurely terminated according to the institutional guidelines. At sacrifice, tissues, including the entire pancreas, other organs and blood will be harvested and processed. The exact post-processing procedures depend on the individual Project requirements and needs. In general, half of the pancreas will be fixed in formalin and embedded in paraffin (for standard histology and immunohistochemistry), the other half frozen in liquid nitrogen (for RNA and protein analysis). A small piece of the pancreas will be used for genotyping (to detect the successful recombination event). Included in the Animal Core is the Pathology Sub-Core, which will provide the histo-pathological evaluation of all mouse tissues. Services that the Animal Core provides for each Project include: Obtaining and maintaining institutional approval, designing the animal studies in terms of logistics and statistical power calculations (together with Project Pi's and Biostatistics Sub-Core), maintaining animal strains and setting up breeding pairs, genotyping of all animals, randomization of animals to experimental groups, preparation and administration of experimental diets or interventions, daily monitoring and animal care, euthanasia of animals, harvest of tissues and blood at sacrifice, processing tissue, e.g. fixing in formalin and embedding in paraffin, and sectioning (together with the Pathology Sub-Core), histopathological evaluation of tissue sections (together with the Pathology Sub- Core), generation of cell cultures (PanlNs and murine pancreatic cancer), storage of raw data, central banking of tissues and biological samples, distribution of tissues to individual Projects, discussion of results with Project Leaders, training of Project investigators in animal procedures (if desired).