MECHANISMS OF BRAIN DYSFUNCTION IN TUBEROUS SCLEROSIS

结节性硬化症脑功能障碍的机制

• 批准号: 8636498
• 负责人: MICHAEL WONG
• 金额: $ 32.26万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-21 至 2015-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8636498
• 关键词: Accounting; Acute; Affect; Antiepileptogenic; Astrocytes; Behavioral; Binding; Brain; Buffers; Cessation of life; Chronic; Data; Development; Epilepsy; Epileptogenesis; Functional disorder; Funding; Gene Silencing; General Population; Genes; Genetic; Grant; Guanosine Triphosphate Phosphohydrolases; High Prevalence; Homologous Gene; Human; Lead; Learning Disabilities; Mediating; Memory; Mental Retardation; Modeling; Molecular; Molecular Abnormality; Morbidity - disease rate; Mus; Mutation; Neuroglia; Neurologic; Neurons; Neurotransmitters; Pathway interactions; Patients; Pentylenetetrazole; Phenotype; Population Research; Potassium Glutamate; Protein Biosynthesis; Proteins; Public Health; Regulation; Research; Seizures; Signal Pathway; Signal Transduction; Staging; Status Epilepticus; TSC1 gene; TSC2 gene; Testing; Therapeutic; Tuberous Sclerosis; Tuberous sclerosis protein complex; Work; cell growth; human TSC1 protein; human TSC2 protein; improved; kainate; mTOR protein; mortality; mouse model; novel; nucleophosmin; potassium ion; prevent; public health relevance; ras Proteins

DESCRIPTION (provided by applicant): Tuberous Sclerosis Complex (TSC) is one of the most common genetic causes of epilepsy. In addition, epilepsy in TSC is typically very severe and intractable to available therapies. Most current treatments for epilepsy are simply symptomatic therapies that may suppress seizures but do not necessarily correct the underlying brain abnormalities causing the epilepsy. Thus, understanding the brain mechanisms causing epilepsy ("epileptogenesis") is necessary to develop more effective, "anti-epileptogenic" treatments for both TSC and non-TSC-related epilepsy. We have previously described a mouse model of TSC that recapitulates many features of human TSC (Tsc1GFAPCKO mice), including severe epilepsy. In the first funding period of this grant, we have described a number of cellular and molecular abnormalities in glia and neurons that contribute to epileptogenesis in these mice, such as astrocyte proliferation, neuronal death, impaired glial buffering of neurotransmitters and potassium ions, and abnormal regulation of specific cell signaling pathways. Most remarkably, we showed that pharmacological inhibition of one of these signaling pathways, the mammalian target of rapamycin (mTOR) pathway, completely prevented the development of epilepsy in Tsc1GFAPCKO mice, representing one of the first demonstrations of a robust anti-epileptogenic effect in any epilepsy model. In this grant renewal application, we propose to extend our previous work, now further characterizing specific TSC-regulated signaling pathways involved in epileptogenesis. Our general hypothesis is that epileptogenesis results primarily from initial abnormalities in specific cell signaling pathways and correction of these signaling abnormalities may prevent epileptogenesis in Tsc1GFAPCKO mice, as well as in other epilepsy models. Findings from this grant should help identify novel mechanisms of epileptogenesis and identify new anti- epileptogenic therapeutic approaches not only for epilepsy in TSC, but potentially for all epilepsy in general.

描述（由申请人提供）：结节性硬化症（TSC）是癫痫最常见的遗传原因之一。此外，TSC患者的癫痫通常非常严重，现有治疗方法难以治疗。目前大多数治疗癫痫的方法都是简单的对症治疗，可能会抑制癫痫发作，但不一定能纠正导致癫痫的潜在大脑异常。因此，了解引起癫痫的大脑机制（“癫痫发生”）对于开发更有效的“抗癫痫”治疗TSC和非TSC相关癫痫是必要的。我们之前已经描述了一种TSC小鼠模型，它概括了人类TSC （Tsc1GFAPCKO小鼠）的许多特征，包括严重癫痫。在该基金的第一个资助期，我们描述了一些胶质细胞和神经元中的细胞和分子异常，这些异常有助于这些小鼠的癫痫发生，例如星形胶质细胞增殖，神经元死亡，神经递质和钾离子的胶质缓冲受损，以及特定细胞信号通路的异常调节。最值得注意的是，我们发现药物抑制其中一条信号通路，即哺乳动物雷帕霉素靶点（mTOR）通路，完全阻止了Tsc1GFAPCKO小鼠癫痫的发展，这是首次证明在任何癫痫模型中具有强大的抗癫痫作用之一。在本次拨款续期申请中，我们建议扩展我们之前的工作，现在进一步表征参与癫痫发生的特定tsc调节信号通路。我们的一般假设是，癫痫发生主要是由特定细胞信号通路的初始异常引起的，纠正这些信号通路异常可能会阻止Tsc1GFAPCKO小鼠以及其他癫痫模型中的癫痫发生。这项资助的研究结果将有助于确定癫痫发生的新机制，并确定新的抗癫痫治疗方法，不仅适用于TSC癫痫，而且可能适用于所有癫痫。