Overcoming Resistance to KIT/PDGFRA Inhibition in GIST

克服 GIST 中对 KIT/PDGFRA 抑制的耐药性

• 批准号: 8933243
• 负责人: JONATHAN Alfred FLETCHER
• 金额: $ 24.03万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-31 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8933243
• 关键词: Basic Science; Biochemical; Biological Assay; Biological Markers; Biopsy; Cell Proliferation; Cells; Clinical; Clinical Research; Clinical Trials; Combined Modality Therapy; Complement; Dana-Farber Cancer Institute; Drug resistance; Event; Exhibits; Exposure to; Extinction (Psychology); Failure; Gene Expression Profile; Gene Mutation; Genes; Genetic; Goals; Human; Imatinib; Imatinib mesylate; Intervention; MAP Kinase Gene; MEK inhibition; MEKs; Malignant Neoplasms; Measurable Disease; Metastatic Lesion; Molecular; Mutation; Oncogenic; Oral; PDGFRA gene; PDGFRB gene; Pathway interactions; Patients; Phase; Phosphoproteins; Phosphotransferases; Property; Receptor Protein-Tyrosine Kinases; Regimen; Research; Resistance; Resistance development; Signal Pathway; Signal Transduction; Specificity; Supporting Cell; Testing; Therapeutic; Translating; Translations; Treatment Efficacy; Validation; Work; Xenograft procedure; anticancer activity; bench to bedside; clinical application; clinically relevant; design; exome sequencing; functional genomics; gain of function; inhibitor/antagonist; insight; kinase inhibitor; mutant; novel; pre-clinical; preclinical study; research clinical testing; resistance mechanism; response; small hairpin RNA; success; therapeutic target; therapy development; tumor

This proposal seeks effective combination therapies that maximize GIST response to KIT/PDGFRA inhibition by concurrently targeting the biologically key MEK/MAPK pathway. Most GISTs express mutant, constitutively activated forms of the KIT or PDGFRA, and we have shown that these formerly untreatable cancers can be palliated in 80% of patients by oral single-agent therapy with imatinib mesylate. However,patients responding to imatinib have persistent measurable disease and generally develop resistance within two years of starting treatment. Therefore, more effective and broader-spectrum therapies are urgently needed. Notably, our preliminary studies show that KIT/PDGFRA imatinib resistance mechanisms vary from patient to patient, and also between metastatic lesions in a given patient, but uniformly rely upon MEK/MAPK signaling to support cell proliferation. In Aim 1, by studying MEK/MAPK signaling and response mechanisms, we will develop clinically-relevant biomarkers and - most importantly - we will identify alternate MEK-dependent therapeutic targets which might have greater specificity, in GIST, compared to MEK. In Aim 2. we will characterize mechanisms of MEKi resistance, since such studies are likely to identify biologically essential regulatory nodes in MEK/MAPK-pathways, which - like those found in Aim 1 - will be candidates as biomakers and therapeutic targets in GIST clinical trials. The collective studies in Aims 1-2, by revealing the scope of MEK/MAPK signaling in GIST, will provide the understanding needed to design more effective and less toxic clinical trials. In Aim 3 we evaluate combination therapies with imatinib and MEKi, as a strategy to inhibit downstream signals from the varied gain-of-function KIT mutations each imatinib-resistant patient, while maintaining imatinib inhibition of nonprogressing GIST subclones. This will be accomplished through a phase l/ll clinical trial of the MEK inhibitor, MEK162, combined with imatinib, in patients showing progression of metastatic GIST on imatinib or sunitinib. Through these studies, we will translate the basic science proposed in this SPORE through to clinical application.

这项建议寻求有效的联合疗法，通过同时靶向生物学上关键的MEK/MAPK途径，最大化GIST对KIT/PDGFRA抑制的反应。大多数GIST表达突变的、成分激活的KIT或PDGFRA，我们已经证明，这些以前无法治疗的癌症可以通过口服甲磺酸伊马替尼单药治疗在80%的患者中得到缓解。然而，对伊马替尼有反应的患者有持续的可测量的疾病，通常在开始治疗的两年内产生耐药性。因此，更有效和更广泛的治疗方法是 急需之物。值得注意的是，我们的初步研究表明KIT/PDGFRA伊马替尼耐药机制 因患者而异，在特定患者的转移灶之间也不同，但统一依赖于 MEK/MAPK信号通路支持细胞增殖。在目标1中，通过研究MEK/MAPK信号和 反应机制，我们将开发与临床相关的生物标记物，最重要的是，我们将 确定替代的MEK依赖治疗靶点，与MEK相比，这些靶点在GIST中可能具有更高的特异性。在Aim 2中，我们将描述Meki耐药的机制，因为这样的研究很可能识别MEK/MAPK通路中生物学上必要的调控节点，这些节点--就像Aim 1中发现的那些一样--将成为GIST临床试验中的生物分子和治疗靶点。AIMS 1-2中的集体研究，通过揭示MEK/MAPK信号在GIST中的范围，将提供 需要理解才能设计出更有效、毒性更低的临床试验。在目标3中，我们评估 与伊马替尼和Meki联合治疗，作为抑制不同来源的下游信号的一种策略 每个伊马替尼耐药患者的功能获得试剂盒突变，同时保持对非进展性伊马替尼的抑制 GIST亚克隆。这将通过在服用伊马替尼或舒尼替尼出现转移性胃肠间质瘤进展的患者中联合应用肌醇激酶抑制剂MEK162和伊马替尼进行L 11期临床试验来实现。通过这些研究，我们将把这一孢子提出的基础科学转化为临床应用。