Neurobiology & Genetics Laboratory Core

神经生物学

• 批准号: 8704221
• 负责人: THOMAS B COOPER
• 金额: $ 20.77万
• 依托单位: NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8704221
• 关键词: Amino Acids; Animals; Autopsy; Biological; Biological Assay; Biological Process; Biological Psychiatry; Body Fluids; Brain; Corticosterone; Data; Data Set; Dexamethasone; Epigenetic Process; Equipment; Exposure to; Gene Expression; Genetic; Hair; Human; Hydrocortisone; Image; Instruction; Laboratories; Measures; Methylation; Neurobiology; Neuropeptides; Neurosecretory Systems; Neurotransmitters; Pharmaceutical Preparations; Plasma; Prolactin; Protocols documentation; Psychotropic Drugs; Resources; Running; Saliva; Sampling; Time; Tissues; Toxicology; Urine; brain tissue; drug of abuse; falls; genetic variant; interest; response; salivary assay; square foot; suicidal behavior

The Neurobiology and Genetics Laboratory Core (NGLC) will carry out specialized assays for multiple projects and thereby insure optimal and comparable reliability across projects. It offers an economy of scale and moves resources as study priorities change. The assays fall into several major domains. Toxicology assays such as ones using brain tissue provide a longer time frame on most recent exposure to psychotropic drugs than body fluids since brain levels tend to be 10-40 times higher. This will be valuable for the postmortem brain studies in Project 1 where drugs may alter the results. The second domain is genetics and epigenetics. Here assays will include gene variants, methylation and gene expression assays. The latter domain is applicable to Projects 1 and 2. The core will assay salivary Cortisol in Project 5 and the responses will also be correlated with findings from Projects 3 and 4. Since Project 6 is looking at how suicidal behavior may be predicted by the large imaging data sets from Projects 3 and 4, it will also consider the impact of the laboratory data from Project 5. Thus, findings from this core are relevant directly or indirectly to every project.

神经生物学和遗传学实验室核心（NGLC）将对多个 项目，从而确保跨项目的最佳和可比的可靠性。它提供了规模经济 并随着研究优先级的变化而移动资源。这些测定分为几个主要领域。毒理学 诸如使用脑组织的测定提供了最近暴露于精神药物的较长时间范围， 药物比体液高，因为大脑水平往往高出10-40倍。这将是有价值的 项目1中的死后大脑研究，药物可能会改变结果。第二个领域是遗传学， 表观遗传学这里的测定将包括基因变异、甲基化和基因表达测定。后者 域适用于项目1和2。核心将在项目5中测定唾液皮质醇， 还将与项目3和项目4的调查结果相关联。既然第六项目是研究自杀行为 可以通过项目3和4的大型成像数据集预测，它还将考虑 项目5的实验室数据。因此，这一核心的调查结果与每个项目直接或间接相关。