Core--Clinical laboratory

核心--临床实验室

• 批准号: 6480790
• 负责人: THOMAS B COOPER
• 金额: $ 17.72万
• 依托单位: NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6480790
• 关键词: biomedical facility; clinical chemistry; drug detection; genotype; human tissue; neurogenetics; neurotransmitters; postmortem; suicide

Clinical Laboratory Core. The CLC comprises an analytical laboratory directed by Mr. T.B. Cooper and a molecular genetic studies laboratory directed by Dr. T. Conrad Gilliam, Head of the Columbia Genome Center. In the latter blood will be collected for genotyping from all Conte Center subjects and DNA will be extracted from postmortem brain tissues of suicides and controls. These samples will be tested for a series of candidate genes related to central serotonin metabolism and include the serotonin transporter, tryptophan hydroxylase and serotonin receptors 5HT/1A, 5HT/1D, 5HT/1E and 5HT2A. The Genome Center will be responsible for development and interpretation of the microarray assays to be run in postmortem brain samples from selected regions to detect differences in gene expression and identify new candidate genes. The promoter region of the 5-HTT from postmortem causes that are phenotypic extremes will be sequenced in a attempt to determine new functional polymorphisms. The analytical laboratory will be responsible for assay of neurotransmitters and metabolites (cortisol, prolactin, HIAA, HVA, MHPG, GABA and glutamate) and neuroendocrine measures (cortisol, prolactin, ACTH, and CRF) in CSF and brain tissue from patients, controls, non-human primates and rodents. Brain tissue samples are being screened for the presence of drugs which effect the serotonergic system. Similarly hair samples are screened for these same drugs to detect long term exposure (months or even years).

临床实验室核心。CLC包括一个由T.B.先生领导的分析实验室。库珀和T.康拉德·吉列姆，哥伦比亚基因组中心主任。在后者中，将从所有Conte中心受试者中收集血液进行基因分型，并从自杀者和对照组的死后脑组织中提取DNA。将对这些样本进行一系列与中枢5-羟色胺代谢相关的候选基因检测，包括5-羟色胺转运蛋白、色氨酸羟化酶和5-羟色胺受体5 HT/1A、5 HT/1D、5 HT/1 E和5 HT 2A。基因组中心将负责开发和解释将在选定区域的死后大脑样本中运行的微阵列分析，以检测基因表达的差异并识别新的候选基因。将对表型极端的死后原因产生的5-HTT启动子区进行测序，以确定新的功能多态性。分析实验室将负责测定患者、对照、非人灵长类动物和啮齿类动物CSF和脑组织中的神经递质和代谢物（皮质醇、催乳素、HIAA、HVA、MHPG、GABA和谷氨酸）以及神经内分泌指标（皮质醇、催乳素、ACTH和CRF）。正在对脑组织样本进行筛选，以确定是否存在影响多巴胺能系统的药物。同样，对头发样本进行这些相同药物的筛查，以检测长期暴露（数月甚至数年）。