Novel wedge-based approach for simultaneous multichannel microscopy

用于同步多通道显微镜的基于楔的新颖方法

• 批准号: 8781277
• 负责人: Samuel Hue-Kay Chung
• 金额: $ 16.03万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8781277
• 关键词: Adherence; Agreement; Area; Benchmarking; Biological; Biological Models; Biological Process; Biomedical Research; Boston; Caenorhabditis elegans; Calcium; Cell Culture Techniques; Color; Contracts; Data; Development; Device or Instrument Development; Devices; Elements; Engineering; Evaluation; Fluorescence Microscopy; Future; Goals; Government; Image; Imaging Device; Investigation; Label; Laboratories; Light; Location; Maintenance; Measurement; Measures; Mental Tests; Microscope; Microscopy; Modeling; Nematoda; Neurons; Optics; Performance; Persons; Phase; Photobleaching; Positioning Attribute; Production; Proteins; Reporting; Research Personnel; Resources; Scientist; Small Business Innovation Research Grant; Spectrum Analysis; Speed; System; Techniques; Technology; Testing; Time; Tissue Engineering; Universities; base; biological research; commercialization; cost; cost effective; design; experience; fluorescence microscope; fluorophore; in vivo; instrumentation; light emission; medical schools; molecular imaging; novel; novel strategies; optical imaging; physical science; prevent; programs; prototype; public health relevance; ratiometric; success; tissue/cell culture; user-friendly

DESCRIPTION (provided by applicant): Physical Sciences Inc. (PSI) and the laboratory of Christopher Gabel at Boston University School of Medicine propose to design, develop, characterize, and demonstrate a novel approach to simultaneous multichannel microscopy that will overcome a number of limitations of existing technologies. Simultaneous multichannel microscopy techniques measure changes in multiple observables that are reported by different wavelengths of light, and these techniques are finding greater application in many areas of biological research as noninvasive measurements become more attainable and desirable. Example applications include in vivo ratiometric neuronal calcium imaging, co-localization of multiple fluorescent proteins and labels, and non-invasive pH measurement. Despite the ubiquity of multichannel imaging and the existence of three current commercial approaches to perform simultaneous multichannel imaging, limitations to each approach prevent full utilization and exploitation of the technique. The long-term objective of this project is to develop a simple, inexpensive commercial device that enables standard single-channel fluorescence microscopes to perform simultaneous multichannel microscopy. This approach can easily scale to higher numbers of channels and reduces photobleaching by simultaneous imaging, advancing the capabilities of fluorescence microscopy. Furthermore, the device contains no moving parts and does not require alignment or specialized expertise to operate, allowing advanced imaging while maintaining user-friendliness. Scientific program areas benefitting directly from the novel approach include molecular imaging, optical imaging and spectroscopy, and tissue engineering. During the Phase I program we propose to fabricate and optically test prototypes of the device to confirm adherence to commercial imaging standards. In particular, prototypes will be constructed for and tested in three locations in conventional microscopes to determine the optimal location and configuration for performance, cost, and convenience. Device performance will be evaluated by imaging standard fluorophores in the nematode worm C. elegans and cell culture and comparing these data to corresponding measurements on conventional multichannel imaging devices. Advanced engineering will be performed in Phase II to reduce device cost, further evaluate it, and develop a path for technology commercialization.

描述（由申请人提供）：Physical Sciences Inc. (PSI)和波士顿大学医学院的Christopher Gabel实验室提出设计，开发，表征和演示一种新的方法来同时进行多通道显微镜检查，该方法将克服现有技术的许多限制。同时多通道显微镜技术测量由不同波长的光报告的多个可观测量的变化，这些技术在生物研究的许多领域中找到了更大的应用，因为非侵入性测量变得更加容易实现和可取。示例应用包括体内比率神经元钙成像、多种荧光蛋白和标记的共定位以及非侵入性pH测量。尽管多通道成像的普遍性和存在三种当前的商业方法来执行同时多通道成像，但是对每种方法的限制阻止了该技术的充分利用和开发。该项目的长期目标是开发一种简单，廉价的商业设备，使标准的单通道荧光显微镜进行同时多通道显微镜。这种方法可以很容易地扩展到更高数量的通道，并通过同时成像减少光漂白，提高荧光显微镜的能力。此外，该设备不包含移动部件，不需要对准或专业知识来操作，允许先进的成像，同时保持用户友好性。直接受益于这种新方法的科学项目领域包括分子成像、光学成像和光谱学以及组织工程。在第一阶段的计划，我们建议制造和光学测试原型的设备，以确认遵守商业成像标准。特别是，原型将在传统显微镜的三个位置构建和测试，以确定性能，成本和便利性的最佳位置和配置。将通过对线虫C中的标准荧光团进行成像来评价器械性能。elegans和细胞培养，并将这些数据与常规多通道成像装置上的相应测量进行比较。第二阶段将进行先进的工程设计，以降低设备成本，进一步评估，并为技术商业化开辟道路。