Multiplex Screening Panel for the Detection of Clostridium botulinum Toxin Genes

项目名称 毒素检测多重筛选小组(肉毒梭菌毒素基因； 食品中葡萄球菌肠毒素基因)：液体微阵列xTAG技术 摘要 食源性疾病暴发和食源性疾病给卫生保健带来了巨大的负担 在美国每年都有这样的系统。而食源性疾病暴发的数量(FBDO) 2008年向疾病预防控制中心报告的人数从1270人(2006年)下降到1034人(2008年)， 住院人数增加，23,152人患病，22人死亡。(1) 由于其自我限制的性质，金黄色葡萄球菌食物中毒大多没有报告。虽然它是 很少有危及生命的、传统上易受伤害的人群，如幼儿和老年人 受影响不成比例，死亡率为4.4%(2)。然而，它的丧失能力的效果是 吸入B型(葡萄球菌肠毒素B-SEB)，一种B类选择剂， 是最令人担忧的。肉毒杆菌神经毒素快速分子筛查工具的研制 基因A和B(机器人A、机器人B)用于诊断和监测目的可能提供更好的 对疾病流行病学的了解；它可以被用来预测是否存在 食物中的功能性毒素可能会导致治疗成功，减少 减少住院治疗，降低肉毒杆菌中毒的死亡风险。 此外，追踪代理人和来源越快，公众的信心就越高， 相信那些负责保障我们食品安全的机构。更重要的是，它将使食物受益 监测和疫情调查不仅在夏威夷进行，而且还为美国提供支持 附属于太平洋岛屿。

Project Title Multiplex Screening Panel for the Detection of Toxins (Clostridium botulinum Toxin Genes ; Staphylococcal Enterotoxin genes) in Food: Liquid MicroArray xTAG Technology Abstract Food borne disease outbreaks and food borne illnesses place a significant load on the health care system in the United States each year. While the number of food borne disease outbreaks (FBDO) reported to the CDC in 2008 have decreased from 1,270 (2006) to 1,034 (2008), the number of hospitalizations have increased where 23,152 became ill and there were 22 reported deaths. (1) Due to its self-limiting nature, Staphyloccocal food-intoxication goes mostly unreported. While it is rarely life-threatening, traditionally vulnerable populations, such as young children and the elderly, are disproportionately affected with a 4.4% mortality rate (2). However, it is the incapacitating effects of inhalational exposure to serotype B (Staphylococcal Enterotoxin B-SEB), a Category B select agent, that is of utmost concern. The development of a rapid molecular tool to screen for botulinum neurotoxin genes A and B ( bot A, bot B) for diagnostic purposes and surveillance may provide a better understanding of the epidemiology of the disease; it may be used as a predictor for the presence of the functional toxins in the food that may lead to successful treatment, reduce the number of hospitalizations and reduce the risk of deaths from botulism. Additionally, the faster an agent and source can be traced, the higher the public's confidence and trust in those agencies responsible for keeping our food safe. More importantly, it will benefit food surveillance and outbreak investigations not only in Hawaii but also to provide support for the US Affiliated Pacific Islands.