Multiplex Screening Panel for the Detection of Clostridium botulinum Toxin Genes

用于检测肉毒杆菌毒素基因的多重筛选小组

• 批准号: 8502396
• 负责人: Andrew C Whelen
• 金额: $ 25万
• 依托单位: HAWAII STATE DEPARTMENT OF HEALTH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-18 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8502396
• 关键词: Multiplex; Screening; Panel; Detection; Clostridium

Project Title Multiplex Screening Panel for the Detection of Toxins (Clostridium botulinum Toxin Genes ; Staphylococcal Enterotoxin genes) in Food: Liquid MicroArray xTAG Technology Abstract Food borne disease outbreaks and food borne illnesses place a significant load on the health care system in the United States each year. While the number of food borne disease outbreaks (FBDO) reported to the CDC in 2008 have decreased from 1,270 (2006) to 1,034 (2008), the number of hospitalizations have increased where 23,152 became ill and there were 22 reported deaths. (1) Due to its self-limiting nature, Staphyloccocal food-intoxication goes mostly unreported. While it is rarely life-threatening, traditionally vulnerable populations, such as young children and the elderly, are disproportionately affected with a 4.4% mortality rate (2). However, it is the incapacitating effects of inhalational exposure to serotype B (Staphylococcal Enterotoxin B-SEB), a Category B select agent, that is of utmost concern. The development of a rapid molecular tool to screen for botulinum neurotoxin genes A and B ( bot A, bot B) for diagnostic purposes and surveillance may provide a better understanding of the epidemiology of the disease; it may be used as a predictor for the presence of the functional toxins in the food that may lead to successful treatment, reduce the number of hospitalizations and reduce the risk of deaths from botulism. Additionally, the faster an agent and source can be traced, the higher the public's confidence and trust in those agencies responsible for keeping our food safe. More importantly, it will benefit food surveillance and outbreak investigations not only in Hawaii but also to provide support for the US Affiliated Pacific Islands.

项目名称 用于检测毒素（肉毒梭菌毒素基因）的多重筛选板; 食品中的葡萄球菌肠毒素基因：液相微阵列xTAG技术 摘要 食源性疾病的爆发和食源性疾病给卫生保健带来了沉重的负担 美国每年的系统。虽然食源性疾病爆发的数量（FBDO） 2008年向疾病预防控制中心报告的病例从1，270例（2006年）减少到1，034例（2008年）， 住院人数增加，有23 152人患病，据报有22人死亡。（一） 由于其自限性，葡萄球菌食物中毒大多未报告。虽然 很少危及生命的传统弱势群体，如幼儿和老年人， 不成比例地受到影响，死亡率为4.4%（2）。然而，这是无能的影响， 吸入暴露于血清型B（葡萄球菌肠毒素B-SE B），一种B类选择因子， 是最令人担忧的肉毒神经毒素快速分子检测方法的建立 基因A和B（bot A，bot B）用于诊断目的和监测可以提供更好的 对疾病流行病学的了解;它可以作为一个预测的存在， 食物中的功能性毒素可能导致成功治疗，减少 住院治疗和减少肉毒杆菌中毒死亡的风险。 此外，追踪代理人和来源的速度越快，公众的信心就越高， 信任那些负责保证我们食品安全的机构。更重要的是， 不仅在夏威夷进行监测和疫情调查，而且还为美国提供支持 附属太平洋岛屿。