Evaluation of the MALDI-TOF-MS for the detection of Listeria monocytogenes in Ready-to-Eat Food Products

MALDI-TOF-MS 对即食食品中单核细胞增生李斯特氏菌检测的评估

• 批准号: 9084884
• 负责人: Andrew C Whelen
• 金额: $ 25.7万
• 依托单位: HAWAII STATE DEPARTMENT OF HEALTH
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9084884
• 关键词: Evaluation; MALDI; TOF; MS; detection

Project Title Evaluation of the Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) for the detection of Listeria monocytogenes in Ready-To-Eat Imported Food Products Abstract Aim: This proposal will evaluate the use of the MALDI-TOF-MS system to detect L. monocytogenes in high-risk foods (HRFs) such as Ready-to-Eat (RTE) imported foods that may be eaten uncooked (e.g., preserved fish, deli meats) or re-heated ( e.g. frankfurters) or eaten raw ( fruits, vegetables like sprouts). L. monocytogenes has the ability to grow in cold storage or refrigerator temperature where RTE food products, imported or locally made (cheese, deli meats, preserved fish, cooked-ready-to-eat crustaceans, etc.) are stored. (3) Method: MALDI-TOF-MS technology will be used to identify L. monocytogenes directly from a selective enrichment broth that has been incubated for 30 hours to allow the organism to grow and multiply in numbers that can be detected without culture and isolation on a plated media. We will demonstrate that the MALDI-TOF-MS system identifies L. monocytogenes through laser desorption ionization of the sample attached to a matrix crystals. This process generates a unique mass spectral pattern or fingerprint that can be used to compare with reference libraries in the MALDI-TOF-MS system database to identify the microorganism. (4) (5) (6) Significance and Impact of the Study: It is critical to have a rapid method to detect L. monocytogenes because of its ability to grow in cold storage or refrigerator temperature where RTE food products, imported or locally made are stored. (3) The HRF designation of RTE foods is based on several factors as defined in Section 204 (d) (2) (A) of the Food Safety Modernization ACT (FSMA). (1) Rapid detection of L. monocytogenes from contaminated RTE imported foods is critical in preventing the distribution of RTE food/food products contaminated with L. monocytogenes that continues to continuous to proliferate/multi[ply while in storage.

项目名称 基质辅助激光解吸电离飞行时间质谱仪的评价 进境即食食品中单核细胞增多性李斯特菌的MALDI-TOF-MS检测 摘要 目的：本提案将评估MALDI-TOF-MS系统在检测单核细胞增多性李斯特菌中的应用。 高危食品(HRF)，例如可生吃的即食(RTE)进口食品(例如， 例如腌制鱼、熟食肉类)或重新加热(例如法兰克福香肠)或生吃(水果、蔬菜，如芽菜)。 单核细胞增多性乳杆菌能够在冷藏或冷藏温度下生长 进口或本地制造的产品(奶酪、熟食肉、腌鱼、即食熟食 甲壳类等)都被储存起来。(3) 方法：将MALDI-TOF-MS技术应用于单核细胞增多性李斯特菌的鉴定 培养30小时的增菌液，以使微生物生长和繁殖 在平板培养基上无需培养和隔离即可检测到的数量。我们会 证明MALDI-TOF-MS系统通过激光解吸鉴定单核细胞增多性李斯特菌 电离附着在基质晶体上的样品。这个过程产生了一个独特的质谱图 可用于与MALDI-TOF-MS系统中的参考库进行比较的图案或指纹 数据库来鉴定微生物。(4)(5)(6) 这项研究的意义和影响：拥有一种快速检测单核细胞增多性李斯特菌的方法至关重要 因为它能够在冷藏或冰箱温度下生长， 进口的或本地制造的都储存起来。(3)RTE食品的HRF指定是基于几个因素 如《食品安全现代化法》(FSMA)第204(D)(2)(A)节所定义。(1)快速 检测受污染的RTE进口食品中的单核细胞增多性李斯特菌对预防 受单核细胞增多性李斯特菌污染的RTE食品/食品的分布情况继续 在储存过程中不断地增殖/复盖。