Seahorse XF96e Metabolic Analyzer purchase

海马XF96e代谢分析仪购买

• 批准号: 8639941
• 负责人: CAROLINE M ALEXANDER
• 金额: $ 15.99万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-15 至 2015-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639941
• 关键词: Biological Assay; Biomedical Research; Cancer Center; Cells; Complement; Equilibrium; Funding; Genus Hippocampus; Glycolysis; Growth; Hospitals; Housing; Institutes; Life; Medical Research; Metabolic; Metabolism; Oxidative Phosphorylation; Pharmacy facility; Phenotype; Public Health; Relative (related person); Request for Proposals; Research Personnel; Running; System; Tumor Stem Cells; Universities; Wisconsin; instrument; medical schools; member; screening; small molecule; stem cell population

DESCRIPTION (provided by applicant): This proposal requests funds for a Seahorse XF96e Metabolic Analyzer. The system will be housed in the Small Molecules Screening and Synthesis Facility at the Wisconsin Institutes for Medical Research in the University of Wisconsin Carbone Cancer Center (UWCCC). It will be available for members of the UWCCC, and more widely, for researchers in the UW Hospital and Pharmacy buildings. Although there have been some 600 Analyzers sold nation-wide to date, this will be the first analyzer to come to the University of Wisconsin School of Medicine and Public Health campus. It represents a unique instrument with the potential to complement or replace the assays that are currently run in our biomedical research labs. This analyzer generates detailed information about the relative rates of glycolysis and oxidative phosphorylation, either at equilibrium, or after perturbation of live cell systems. With the re-emergence of metabolism as a key regulator of growth, not only of tumors, but stem cell populations, this instrument will be key to the interpretation of their phenotypes.

描述（由申请人提供）：该提案要求为 Seahorse XF96e 代谢分析仪提供资金。该系统将安装在威斯康星大学卡本癌症​​中心 (UWCCC) 威斯康星医学研究所的小分子筛选和合成设施中。它将供 UWCCC 成员使用，更广泛地供威斯康星大学医院和药房大楼的研究人员使用。尽管迄今为止已在全国范围内销售了约 600 台分析仪，但这将是威斯康星大学医学与公共卫生学院校园的第一台分析仪。它代表了一种独特的仪器，有可能补充或取代我们生物医学研究实验室目前运行的检测方法。该分析仪可生成有关平衡时或活细胞系统扰动后糖酵解和氧化磷酸化相对速率的详细信息。随着新陈代谢重新成为肿瘤和干细胞群生长的关键调节因子，该仪器将成为解释其表型的关键。