Seahorse XF96e Metabolic Analyzer purchase

描述（由申请人提供）：本提案要求为Seahorse XF96e代谢分析仪提供资金。该系统将被安置在威斯康星州大学Carbone癌症中心（UWCCC）的威斯康星州医学研究所的小分子筛选和合成设施中。它将提供给UWCCC的成员，更广泛地说，在UW医院和药房大楼的研究人员。虽然迄今为止全国范围内已经销售了大约600台分析仪，但这将是第一台进入威斯康星州大学医学和公共卫生学院的分析仪。它代表了一种独特的仪器，有可能补充或取代目前在我们的生物医学研究实验室中运行的测定。该分析仪生成有关糖酵解和氧化磷酸化的相对速率的详细信息，无论是在平衡状态下，还是在活细胞系统扰动后。随着新陈代谢作为生长的关键调节因子的重新出现，不仅是肿瘤，而且是干细胞群，这种仪器将成为解释其表型的关键。

DESCRIPTION (provided by applicant): This proposal requests funds for a Seahorse XF96e Metabolic Analyzer. The system will be housed in the Small Molecules Screening and Synthesis Facility at the Wisconsin Institutes for Medical Research in the University of Wisconsin Carbone Cancer Center (UWCCC). It will be available for members of the UWCCC, and more widely, for researchers in the UW Hospital and Pharmacy buildings. Although there have been some 600 Analyzers sold nation-wide to date, this will be the first analyzer to come to the University of Wisconsin School of Medicine and Public Health campus. It represents a unique instrument with the potential to complement or replace the assays that are currently run in our biomedical research labs. This analyzer generates detailed information about the relative rates of glycolysis and oxidative phosphorylation, either at equilibrium, or after perturbation of live cell systems. With the re-emergence of metabolism as a key regulator of growth, not only of tumors, but stem cell populations, this instrument will be key to the interpretation of their phenotypes.