Transposons, RNA Interference and Heterochromatin

转座子、RNA 干扰和异染色质

• 批准号: 8720010
• 负责人: ROBERT A MARTIENSSEN
• 金额: $ 34.61万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8720010
• 关键词: Animals; Biogenesis; Cell Nucleus; Cells; Cereals; Companions; DNA; DNA Methylation; DNA Transposable Elements; Defect; Development; Diploidy; Embryo; Environmental Risk Factor; Epigenetic Process; Flowers; Gene Expression; Genes; Genetic; Genome; Germ Cells; Health; Heterochromatin; Histones; Homologous Gene; Human; Inherited; Lead; Meiosis; Methylation; MicroRNAs; Mitotic; Modification; Mus; Nurses; Pathway interactions; Phenotype; Plants; Play; Pollen; Production; RNA Interference; Reproduction; Role; Seeds; Signal Transduction; Small Interfering RNA; Small RNA; Somatic Cell; Structure of primordial sex cell; Supporting Cell; Tumor Suppressor Genes; Variant; cancer genome; chromatin remodeling; egg; flower pollen; imprint; mutant; next generation; pluripotency; sperm cell; transmission process; zygote

DESCRIPTION (provided by applicant): The germlines of plants and animals undergo genome reprogramming in order to reset epigenetic marks that would otherwise interfere with pluripotency of the zygote. Perhaps chief among these marks are epigenetic modifications of transposable elements (TE), which make up a majority of most eukaryotic genomes. We have found that small interfering RNA derived from heterochromatin plays a key role in germline reprogramming in plants, and there is mounting evidence for a similar phenomenon in animals. Germ cells in plants arise from the products of meiosis by mitotic division, and a number of companion cells also differentiate resembling nurse cells and other support cells in animals. We have found that reprogramming of the pollen grain companion cell nucleus (the vegetative nucleus or VN) results in transposon activation, and that a new class of "epigenetically activated" small interfering RNA (easiRNA) accumulate in sperm cells. In ovules, mutants in this same small RNA pathway result in differentiation of additional ameiotic diploid germ cells, as well as TE activation in the egg. Aberrant germ cell specification has profound implications for plant reproduction, including the production of clonal unreduced seeds (apomixis). We will investigate the mechanism of germline reprogramming in plants, and the function of small RNA in specification of germ cell identity. We will also investigate the transgenerational inheritance of epialleles when reprogramming goes awry. We have preliminary evidence that systemically mobile small RNA accumulate in the gametes, from which they are passed into the fertilized zygote, providing a mechanism for transgenerational inheritance.

描述（由申请人提供）：植物和动物的生殖系经历基因组重编程，以重置表观遗传标记，否则表观遗传标记会干扰合子的多能性。这些标记中最主要的可能是转座因子（TE）的表观遗传修饰，它构成了大多数真核生物基因组的大部分。我们已经发现，来自异染色质的小干扰RNA在植物的生殖细胞重编程中起着关键作用，并且有越来越多的证据表明在动物中存在类似的现象。植物中的生殖细胞是由有丝分裂的减数分裂产物产生的，许多伴细胞也分化，类似于动物中的营养细胞和其他支持细胞。我们已经发现，花粉粒伴随细胞核（营养核或VN）的重编程导致转座子激活，并且一类新的“表观遗传激活”小干扰RNA（siRNA）在精细胞中积累。在胚珠中，相同的小RNA途径中的突变体导致额外的非减数分裂二倍体生殖细胞的分化，以及卵中的TE激活。异常生殖细胞特化对植物生殖，包括无性系未减数种子（无融合生殖）的产生具有深远的影响。我们将研究植物生殖细胞重编程的机制，以及小RNA在生殖细胞身份特化中的功能。我们还将研究当重编程出错时表观等位基因的跨代遗传。我们有初步证据表明，系统性移动的小RNA积累在配子中，从那里它们被传递到受精卵，提供了一个跨代遗传的机制。