Molecular Core

分子核心

• 批准号: 9703532
• 负责人: SVEN-ERIC JORDT
• 金额: $ 31.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-20 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9703532
• 关键词: Acupuncture Points; Analgesics; Astrocytes; Behavioral; Biological Markers; Blinded; Blood; Blood Circulation; Brain; Brain Stem; Cell Culture Techniques; Cells; Cerebrospinal Fluid; Chronic; Core Facility; Dependovirus; Detection; Ear; Enzyme-Linked Immunosorbent Assay; Evaluation; Experimental Designs; Feedback; Flow Cytometry; Fracture; Gene Expression; Gene Expression Profiling; Genes; Genetic Transcription; Guidelines; Immunoassay; Immunologics; Individual; Inflammation; Inflammatory; Injury; Intervention; Label; Leukocytes; Liquid substance; Maps; Measures; Methods; Modeling; Molecular; Mus; Neurons; Neuropeptides; Nociceptors; Organ; Pain; Pathway interactions; Peripheral; Persistent pain; Population; Postoperative Pain; Production; RNA; Recovery; Research Personnel; Resolution; Resources; Sampling; Sensory Ganglia; Site; Spinal; Spinal Cord; Stimulus; Stress; Subfamily lentivirinae; Syndrome; Therapeutic; Therapeutic Intervention; Tibial Fractures; Tissue Sample; Tissues; Tracer; Transcript; Trigeminal Pain; Trigeminal System; Variant; Viral; Virus Diseases; Visual; Work; afferent nerve; chemokine; chemotherapy; cytokine; data exchange; injured; lipid mediator; lipoxin A4; nerve injury; neuroinflammation; neuronal circuitry; pain model; pain signal; tool

Molecular Core – Summary The molecular and immunological characterization of inflammation and injury markers is crucial for the evaluation of the analgesic complementary approaches proposed in all four projects of this Center. Preliminary studies have identified sets of pro-inflammatory genes in sensory ganglia, spinal cord, brainstem and brain strongly induced by the pro-algesic stimuli in the models (chemotherapy, tibial fracture, functional pain syndrome, trigeminal pain) suggesting that pain has detrimental effects on the function of the CNS and induces inflammatory states in other organs remote from the site of injury. The Molecular Core will support the Center Projects through the following Specific Aims: Core Specific Aim 1: Analyze the effects of injuries, prior stress and complementary analgesic interventions on gene expression in injured tissues and neuronal tissues of the pain pathway. Core Specific Aim 2: Determine systemic and tissue levels of inflammatory biomarkers and inflammation-resolving lipid mediators. Core Specific Aim 3: Examine the effects of pain stimuli, prior stress and complementary interventions on systemic leukocyte profiles and neuroinflammatory cells in neuronal tissues. Core Specific Aim 4:Provide resources for production of viral tracers for labeling of neuronal circuits engaged in pain models and by bioelectronic complementary analgesic interventions. Methods include quantitative PCR analysis, multiplex immunoassays and ELISAs, flow cytometry and cell culture. The Molecular Core will work closely together with Project Investigators, Behavioral Core, the Statistical Unit of the Administrative Core and external core facilities to establish a workflow for sample tracing, blinded data transfer and feedback for optimization of experimental designs.

分子核心-摘要 炎症和损伤标志物的分子和免疫学特征对于评估是至关重要的 在该中心的所有四个项目中提出的止痛辅助方法。初步研究表明 在感觉神经节、脊髓、脑干和脑中发现强烈诱导的促炎基因集 通过模型中的前痛刺激(化疗、胫骨骨折、功能性疼痛综合征、三叉神经痛) 这表明疼痛对中枢神经系统的功能有不利影响，并在其他部位引起炎症状态 远离受伤部位的器官。分子核心将通过以下方式支持该中心的项目 具体目标：核心具体目标1：分析损伤、先前应激和补充止痛药的影响 对疼痛通路损伤组织和神经元组织基因表达的干预。核心特定 目的2：测定全身和组织中炎症生物标志物和消炎脂的水平 调解人。核心具体目标3：检查疼痛刺激、先前应激和互补的影响 干预对神经组织中系统性白细胞谱和神经炎性细胞的影响。核心特定 目的4：为制作病毒示踪剂提供资源，用于标记参与疼痛模型的神经元回路 并通过生物电子辅助止痛干预。方法包括定量PCR分析， 多重免疫分析和ELISA、流式细胞术和细胞培养。分子核心将密切合作 与项目调查员、行为核心、行政核心统计股和外部 核心设施，以建立样本跟踪、盲目数据传输和反馈的工作流程，以优化 实验设计。