Molecular Core

分子核心

• 批准号: 9703532
• 负责人: SVEN-ERIC JORDT
• 金额: $ 31.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-20 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9703532
• 关键词: Acupuncture Points; Analgesics; Astrocytes; Behavioral; Biological Markers; Blinded; Blood; Blood Circulation; Brain; Brain Stem; Cell Culture Techniques; Cells; Cerebrospinal Fluid; Chronic; Core Facility; Dependovirus; Detection; Ear; Enzyme-Linked Immunosorbent Assay; Evaluation; Experimental Designs; Feedback; Flow Cytometry; Fracture; Gene Expression; Gene Expression Profiling; Genes; Genetic Transcription; Guidelines; Immunoassay; Immunologics; Individual; Inflammation; Inflammatory; Injury; Intervention; Label; Leukocytes; Liquid substance; Maps; Measures; Methods; Modeling; Molecular; Mus; Neurons; Neuropeptides; Nociceptors; Organ; Pain; Pathway interactions; Peripheral; Persistent pain; Population; Postoperative Pain; Production; RNA; Recovery; Research Personnel; Resolution; Resources; Sampling; Sensory Ganglia; Site; Spinal; Spinal Cord; Stimulus; Stress; Subfamily lentivirinae; Syndrome; Therapeutic; Therapeutic Intervention; Tibial Fractures; Tissue Sample; Tissues; Tracer; Transcript; Trigeminal Pain; Trigeminal System; Variant; Viral; Virus Diseases; Visual; Work; afferent nerve; chemokine; chemotherapy; cytokine; data exchange; injured; lipid mediator; lipoxin A4; nerve injury; neuroinflammation; neuronal circuitry; pain model; pain signal; tool

Molecular Core – Summary The molecular and immunological characterization of inflammation and injury markers is crucial for the evaluation of the analgesic complementary approaches proposed in all four projects of this Center. Preliminary studies have identified sets of pro-inflammatory genes in sensory ganglia, spinal cord, brainstem and brain strongly induced by the pro-algesic stimuli in the models (chemotherapy, tibial fracture, functional pain syndrome, trigeminal pain) suggesting that pain has detrimental effects on the function of the CNS and induces inflammatory states in other organs remote from the site of injury. The Molecular Core will support the Center Projects through the following Specific Aims: Core Specific Aim 1: Analyze the effects of injuries, prior stress and complementary analgesic interventions on gene expression in injured tissues and neuronal tissues of the pain pathway. Core Specific Aim 2: Determine systemic and tissue levels of inflammatory biomarkers and inflammation-resolving lipid mediators. Core Specific Aim 3: Examine the effects of pain stimuli, prior stress and complementary interventions on systemic leukocyte profiles and neuroinflammatory cells in neuronal tissues. Core Specific Aim 4:Provide resources for production of viral tracers for labeling of neuronal circuits engaged in pain models and by bioelectronic complementary analgesic interventions. Methods include quantitative PCR analysis, multiplex immunoassays and ELISAs, flow cytometry and cell culture. The Molecular Core will work closely together with Project Investigators, Behavioral Core, the Statistical Unit of the Administrative Core and external core facilities to establish a workflow for sample tracing, blinded data transfer and feedback for optimization of experimental designs.

分子核心——总结 炎症和损伤标志物的分子和免疫学特征对于评估至关重要 该中心所有四个项目中提出的镇痛补充方法。初步研究有 确定了感觉神经节、脊髓、脑干和脑强诱导的促炎基因组 通过模型中的促痛刺激（化疗、胫骨骨折、功能性疼痛综合征、三叉神经痛） 表明疼痛对中枢神经系统功能有不利影响，并会引起其他部位的炎症状态 远离受伤部位的器官。分子核心将通过以下方式支持中心项目 具体目标： 核心具体目标 1：分析受伤、先前压力和补充镇痛药的影响 对疼痛通路受损组织和神经元组织的基因表达进行干预。核心特定 目标 2：确定炎症生物标志物和炎症缓解脂质的全身和组织水平 调解员。核心具体目标 3：检查疼痛刺激、先前压力和补充的影响 对神经组织中全身白细胞谱和神经炎症细胞的干预。核心特定 目标 4：为病毒示踪剂的生产提供资源，用于标记参与疼痛模型的神经元回路 以及生物电子补充镇痛干预措施。方法包括定量 PCR 分析、 多重免疫测定和 ELISA、流式细胞术和细胞培养。分子核心将紧密合作 与项目调查员、行为核心、行政核心统计单位和外部 建立样本追踪、盲数据传输和反馈优化工作流程的核心设施 实验设计。