Lung-on-a-chip enables dynamic imaging of pulmonary lung tissue in response to aerosolized nanoparticles

项目摘要 纳米颗粒正逐渐用作消费品和治疗剂， 作为环境毒素正在流行。人们越来越担心， 纳米颗粒对人类健康的影响，特别是肺部。肺部是最易受感染的区域 与环境微粒相互作用。纳米粒子相互作用的研究现状 对导致炎症和细胞死亡的机制缺乏了解。 静态单一培养中的纳米颗粒暴露可能无法预测复杂的生化反应 在人的肺里触发。昂贵的，道德上受到挑战的动物模型，缺乏能力 进行机械研究。然而，肺芯片微流体平台提供越来越多的 复杂的方法来研究体内肺反应。这些平台通过应用 对内皮细胞和分化上皮细胞共培养物的流体动力和机械力 以模拟肺泡-毛细血管界面内的生理环境。有一个 越来越需要使用这些动态平台来研究纳米颗粒对肺的相互作用 组织.为此，我制造的肺芯片平台能够监测细胞间液 渗漏和活细胞成像。有了这些工具，项目 试图使用动态成像来研究雾化纳米颗粒对肺组织的影响。 将监测组织的炎症进展和纳米颗粒迁移。

Project Summary Nanoparticles are moving towards use as consumer products and therapeutic agents, and are gaining prevalence as environmental toxins. There is increasing concern on the impact that nanoparticles have on human health, specifically the lung. The lung is the most susceptible region of the body to interact with environmental particulates. Current studies of nanoparticle interaction with cells lacks insight on the mechanisms that results in inflammation and cell death. Nanoparticle exposure in static monocultures may not predict the complex biochemical responses triggered in the human lung. Expensive, ethically challenged animal models, lack the capability for mechanistic studies. Whereas, lung-on-a-chip microfluidic platforms offer increasingly sophisticated methods to study the in vivo lung response. These platforms do so by applying hydrodynamic and mechanical forces to cocultures of endothelial and differentiated epithelial cells to mimic the physiological environment within the alveolar-capillary interface. There is an increasing need to use these dynamic platforms to study the interaction of nanoparticles on lung tissue. To this end, my fabricated lung-on-a-chip platform enables monitoring intercellular fluid leakage and live cell imaging in response to induced inflammation. With these tools, the project seeks to use dynamic imaging to study the impact of aerosolized nanoparticles on lung tissue. The tissue will be monitored for the progression of inflammation and nanoparticle migration.