Early Life Experience Imprints Gut Microbiome in Preterm Infants

早期生活经历影响早产儿肠道微生物组

• 批准号: 8743310
• 负责人: Xiaomei Sophia Cong
• 金额: $ 13.24万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-27 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8743310
• 关键词: Address; Advisory Committees; Applications Grants; Area; Automobile Driving; Autonomic nervous system; Award; Bacteria; Bacterial Antigens; Base Sequence; Biological; Biological Markers; Biometry; Brain; Caring; Clinical Data; Clinical Investigator; Communication; Data; Data Analyses; Disease; Doctor of Philosophy; Educational workshop; Emotional Stress; Environment; Enzyme-Linked Immunosorbent Assay; Epithelial; Etiology; Family; Gastrointestinal tract structure; Genomics; Goals; Grant; Health; Health protection; Human Microbiome; Immune; Immune system; Infant; Infant Development; Infant Health; Infection; Inflammatory; Inflammatory Response; Informatics; Knowledge; Lead; Learning Module; Leukocyte L1 Antigen Complex; Life; Life Experience; Life Stress; Longitudinal Studies; Measures; Mentored Patient-Oriented Research Career Development Award; Mentors; Methods; Modeling; Modification; Morbidity - disease rate; Neonatal; Neonatal Intensive Care; Neonatal Intensive Care Units; Nervous System Trauma; Neuraxis; Neurologic; Nurses; Outcome; Pain; Pattern; Permeability; Play; Pregnancy; Premature Birth; Premature Infant; Prevention; Procedures; Process; Quality of life; Regulation; Research; Research Training; Role; Sampling; Scientist; Signal Transduction; Specific qualifier value; Strategic Planning; Stress; Survivors; System; Technology; Testing; Time; Training; United States National Institutes of Health; Vulnerable Populations; Work; Writing; adverse outcome; base; career; caregiving; cell motility; cost; cytokine; disorder prevention; early childhood; experience; gut microbiota; heart rate variability; high risk; high risk infant; hypothalamic-pituitary-adrenal axis; imprint; improved; infancy; maternal separation; metagenomic sequencing; microbial; microbiome; model development; neurobehavioral; neurodevelopment; patient oriented; premature; programs; prospective; public health relevance; rRNA Genes; research study; response; skills; stressor

DESCRIPTION (provided by applicant): The candidate, Xiaomei Cong, PhD, RN, proposes a Mentored Patient-Oriented Research Career Development Award project to enhance her ability to achieve her goal of becoming an independent clinical investigator who will lead the field of infant stress and pain. With preterm birth substantial gains in survival, concerns have focused on significant increases in neurological morbidity and long-term adverse outcomes related to the immature neuro-immune systems and stressful early life experience in the neonatal intensive care (NICU) stay. However, how the mechanisms of early life stress alter infant neurodevelopment remains largely unknown. To continue progress towards her goal, the candidate will examine the new models of brain- gut-microbiota signaling mechanism involved in early life stress and prediction of infant neurodevelopment using cutting-edge microbiological analysis (i.e., gut microbiome) and immune-inflammatory biomarker prediction strategies (i.e., fecal cytokines and calprotectin). Three specific research aims are proposed in a prospective longitudinal study to examine: (1) preterm infants' gut microbiome patterns and mucosal immune- inflammatory responses over the first 3 weeks of NICU stay; (2) the linkage of gut microbiome patterns and mucosal immune-inflammatory responses with early life stress; and (3) the linkage of gut microbiome and mucosal immune-inflammatory responses with autonomic nervous and neurobehavioral outcomes. The candidate's previous training and research experience provided her skills to plan, implement, and analyze data from clinical experimental studies in infant stress/pain. Progress toward the career goals requires her to develop expertise in additional content areas including knowledge and technology of brain-gut signaling mechanism and genomic approaches to microbiome analysis. An expert interdisciplinary mentor advisory committee has been selected and the candidate will gain knowledge by hands-on experience of: (1) microbiome and genomics knowledge and technology by Drs. Graf and Gregory; (2) mechanism of brain-gut signaling involved in infant stress by Drs. Henderson and Hussain; (3) biostatistics and informatics by Drs. Walsh and Graf; and (4) dissemination and grant writing by all mentors. Training and mentoring activities will include formal coursework, learning modules, mentored training, workshops, and other professional activities. The project addresses a high priority of the NINR strategic plan of disease prevention and quality of life improvement and also a priority of NIH specified by a recent initiative, the "Human Microbiome Project." The award will enable 75% of her academic time to be devoted to research and training. The candidate will work with mentors in a world-class research environment and successfully reach her goals. Study results will provide the preliminary data for an R01 grant proposal and will further contribute to neonatal care in reducing negative consequences of stress in high-risk infants. .

描述(申请人提供)：候选人丛晓梅，博士，RN，提出了一个以患者为导向的研究职业发展奖指导项目，以增强她的能力，以实现她的目标，成为一名独立的临床调查员，将领导婴儿压力和疼痛领域。随着早产存活率的大幅提高，人们关注的焦点是与未成熟的神经免疫系统和新生儿重症监护(NICU)住院期间应激早期生活经历相关的神经系统发病率和长期不良后果的显著增加。然而，早期生活压力的机制如何改变婴儿的神经发育在很大程度上仍不清楚。为了继续朝着她的目标前进，候选人将使用尖端微生物学分析(即肠道微生物组)和免疫炎症生物标记物预测策略(即粪便细胞因子和钙保护素)，研究与早期生活应激和婴儿神经发育预测有关的脑-肠道-微生物区系信号机制的新模型。在一项前瞻性的纵向研究中提出了三个具体的研究目标：(1)在NICU住院的前3周内早产儿的肠道微生物群和粘膜免疫炎症反应；(2)肠道微生物群和粘膜免疫炎症反应与早期生活应激的联系；(3)肠道微生物群和粘膜免疫炎症反应与自主神经和神经行为结果的联系。应聘者之前的培训和研究经验为她提供了计划、实施和分析婴儿压力/疼痛临床实验研究数据的技能。为了实现职业目标，她需要在其他内容领域发展专业知识，包括大脑-肠道信号机制和微生物组分析基因组方法的知识和技术。已经选出了一个跨学科的专家导师咨询委员会，候选人将通过实践经验获得以下方面的知识：(1)Graf博士和Gregory博士的微生物组和基因组学知识和技术；(2)Henderson博士和Hussain博士的大脑-肠道信号与婴儿应激相关的机制；(3)Walsh博士和Graf博士的生物统计学和信息学；以及(4)所有导师的传播和赠款撰写。培训和辅导活动将包括正式的课程作业、学习模块、辅导培训、研讨会和其他专业活动。该项目涉及NINR疾病预防和生活质量改善战略计划的高度优先事项，也是最近的一项倡议--“人类微生物组项目”--指定的NIH的优先事项。该奖项将使她75%的学术时间投入到研究和培训中。候选人将在世界级的研究环境中与导师合作，并成功实现她的目标。研究结果将为R01赠款提案提供初步数据，并将进一步有助于新生儿护理，减少高危婴儿应激的负面后果。。