Early Life Experience Imprints Gut Microbiome in Preterm Infants

早期生活经历影响早产儿肠道微生物组

• 批准号: 8618770
• 负责人: Xiaomei Sophia Cong
• 金额: $ 13.15万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-27 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8618770
• 关键词: Address; Advisory Committees; Applications Grants; Area; Automobile Driving; Autonomic nervous system; Award; Bacteria; Bacterial Antigens; Base Sequence; Biological; Biological Markers; Biometry; Brain; Caring; Clinical Data; Clinical Investigator; Communication; Data; Data Analyses; Disease; Doctor of Philosophy; Educational workshop; Emotional Stress; Environment; Enzyme-Linked Immunosorbent Assay; Epithelial; Etiology; Family; Gastrointestinal tract structure; Genomics; Goals; Grant; Health; Health protection; Human Microbiome; Immune; Immune system; Infant; Infant Development; Infant Health; Infection; Inflammatory; Inflammatory Response; Informatics; Knowledge; Lead; Learning; Leukocyte L1 Antigen Complex; Life; Life Experience; Life Stress; Longitudinal Studies; Measures; Mentored Patient-Oriented Research Career Development Award; Mentors; Methods; Modeling; Modification; Morbidity - disease rate; Neonatal; Neonatal Intensive Care; Neonatal Intensive Care Units; Nervous System Trauma; Neuraxis; Neurologic; Nurses; Outcome; Pain; Pattern; Permeability; Play; Pregnancy; Premature Birth; Premature Infant; Prevention; Procedures; Process; Quality of life; Regulation; Research; Research Training; Role; Sampling; Scientist; Signal Transduction; Specific qualifier value; Strategic Planning; Stress; Survivors; System; Technology; Testing; Time; Training; United States National Institutes of Health; Vulnerable Populations; Work; Writing; adverse outcome; base; career; caregiving; cell motility; cost; cytokine; disorder prevention; early childhood; experience; gut microbiota; heart rate variability; high risk; high risk infant; hypothalamic-pituitary-adrenal axis; imprint; improved; infancy; maternal separation; metagenomic sequencing; microbial; microbiome; model development; neurobehavioral; neurodevelopment; patient oriented; premature; programs; prospective; public health relevance; rRNA Genes; research study; response; skills; stressor

Abstract The candidate, Xiaomei Cong, PhD, RN, proposes a Mentored Patient-Oriented Research Career Development Award project to enhance her ability to achieve her goal of becoming an independent clinical investigator who will lead the field of infant stress and pain. With preterm birth substantial gains in survival, concerns have focused on significant increases in neurological morbidity and long-term adverse outcomes related to the immature neuro-immune systems and stressful early life experience in the neonatal intensive care (NICU) stay. However, how the mechanisms of early life stress alter infant neurodevelopment remains largely unknown. To continue progress towards her goal, the candidate will examine the new models of brain- gut-microbiota signaling mechanism involved in early life stress and prediction of infant neurodevelopment using cutting-edge microbiological analysis (i.e., gut microbiome) and immune-inflammatory biomarker prediction strategies (i.e., fecal cytokines and calprotectin). Three specific research aims are proposed in a prospective longitudinal study to examine: (1) preterm infants' gut microbiome patterns and mucosal immune- inflammatory responses over the first 3 weeks of NICU stay; (2) the linkage of gut microbiome patterns and mucosal immune-inflammatory responses with early life stress; and (3) the linkage of gut microbiome and mucosal immune-inflammatory responses with autonomic nervous and neurobehavioral outcomes. The candidate's previous training and research experience provided her skills to plan, implement, and analyze data from clinical experimental studies in infant stress/pain. Progress toward the career goals requires her to develop expertise in additional content areas including knowledge and technology of brain-gut signaling mechanism and genomic approaches to microbiome analysis. An expert interdisciplinary mentor advisory committee has been selected and the candidate will gain knowledge by hands-on experience of: (1) microbiome and genomics knowledge and technology by Drs. Graf and Gregory; (2) mechanism of brain-gut signaling involved in infant stress by Drs. Henderson and Hussain; (3) biostatistics and informatics by Drs. Walsh and Graf; and (4) dissemination and grant writing by all mentors. Training and mentoring activities will include formal coursework, learning modules, mentored training, workshops, and other professional activities. The project addresses a high priority of the NINR strategic plan of disease prevention and quality of life improvement and also a priority of NIH specified by a recent initiative, the "Human Microbiome Project." The award will enable 75% of her academic time to be devoted to research and training. The candidate will work with mentors in a world-class research environment and successfully reach her goals. Study results will provide the preliminary data for an R01 grant proposal and will further contribute to neonatal care in reducing negative consequences of stress in high-risk infants. .

抽象的 候选人丛晓梅（Xiaomei Cong）博士、注册护士提出了以患者为导向的指导性研究职业 发展奖项目旨在增强她实现成为独立临床医生的目标的能力 研究人员将领导婴儿压力和疼痛领域。随着早产的生存率大幅提高， 人们的担忧集中在神经发病率和长期不良后果的显着增加 与神经免疫系统不成熟和新生儿重症监护室早期生活压力经历有关 护理 (NICU) 住院。然而，早期生活压力如何改变婴儿神经发育的机制仍然存在 很大程度上不为人知。为了继续实现她的目标，候选人将检查新的大脑模型 肠道微生物信号传导机制参与早期生活压力和婴儿神经发育的预测 使用尖端微生物分析（即肠道微生物组）和免疫炎症生物标志物 预测策略（即粪便细胞因子和钙卫蛋白）。报告中提出了三个具体研究目标 前瞻性纵向研究旨在检查：（1）早产儿肠道微生物组模式和粘膜免疫 NICU 住院前 3 周的炎症反应； (2) 肠道微生物组模式与 早期生活压力引起的粘膜免疫炎症反应； (3) 肠道微生物组与 粘膜免疫炎症反应与自主神经和神经行为结果。 候选人之前的培训和研究经验为她提供了规划、实施和实施的技能。 分析婴儿压力/疼痛的临床实验研究数据。实现职业目标需要取得进展 她将发展其他内容领域的专业知识，包括脑肠信号传导的知识和技术 微生物组分析的机制和基因组方法。专家跨学科导师咨询 委员会已选定，候选人将通过以下方面的实践经验获得知识：(1) 博士的微生物组和基因组学知识和技术格拉夫和格雷戈里； (2)脑肠机制 博士研究涉及婴儿压力的信号传导。亨德森和侯赛因； (3) 博士的生物统计学和信息学。 沃尔什和格拉夫； (4) 所有导师的传播和资助撰写。培训和指导活动将 包括正式课程、学习模块、指导培训、研讨会和其他专业活动。 该项目解决了 NINR 疾病预防和生活质量战略计划的高度优先事项 改进也是 NIH 最近的一项倡议“人类微生物组项目”指定的优先事项。这 该奖项将使她 75% 的学术时间能够投入到研究和培训中。候选人将工作 在世界一流的研究环境中与导师一起并成功实现她的目标。研究结果将 为 R01 拨款提案提供初步数据，并将进一步为新生儿护理做出贡献，减少 高危婴儿压力的负面后果。 。