Esophageal cancer genetic project

食管癌基因工程

• 批准号: 8938284
• 负责人: Philip Taylor
• 金额: $ 81.39万
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8938284
• 关键词: Area; Biological Specimen Banks; Blood; Blood specimen; Cancer-Predisposing Gene; Case-Control Studies; Cells; County; DNA; Disease; Etiology; Family; Family Study; Fingers; Genetic; Genetic Markers; Genetic Polymorphism; Genomics; Goals; Hot Spot; Individual; Lesion; Malignant Neoplasms; Malignant neoplasm of esophagus; Microsatellite Repeats; Molecular; Normal Range; Population; Premalignant; Risk; Role; Sampling; Specimen; Testing; Venous; cancer genetics; candidate marker; density; esophageal cancer prevention; gene environment interaction; genome wide association study; high risk; malignant stomach neoplasm; stomach cardia; tumor

The overall goal of this project is to understand the role of genetics in the etiology and prevention of esophageal cancer and gastric cancer. Five different but complementary approaches have been used to identify esophageal cancer susceptibility genes. First, a tumor/nontumor study has been conducted in which a biological specimen bank consisting of samples (tumor, nontumor, venous blood, finger stick blood, and buccal cells) from several hundred cases of esophageal cancer and gastric cancer (both cardia and body) was developed in Taiyuan for the identification of esophageal cancer and gastric cancer susceptibility genes and potential early genetic markers of these cancers. High-density genome-wide scans with microsatellite markers have been conducted to identify potential hot spots, and further testing of these hot spots and other candidate markers in additional tumor/nontumor samples is in progress. Premalignant morphologic lesions will also be examined. Second, blood samples for DNA have been collected from several hundred healthy individuals from high-risk (Yangcheng County) and low-risk (Beijing) areas to examine potential population differences in polymorphisms for selected genomic markers. Third, a large case-control study with cases of esophageal cancer and gastric cancer (both cardia and body) has been conducted and is being used to evaluate polymorphisms in the candidate markers identified in other components of this project, and to evaluate gene-environment interactions. Fourth, a family study is in progress which will permit linkage of candidate markers with cancer in families having 2 or more cases with esophageal cancer or gastric cancer. Finally, an endoscopic study is being conducted to obtain specimens from a morphologic spectrum of disease ranging from normal to early invasive esophageal cancer in order to characterize molecular progression.

该项目的总体目标是了解遗传学在食管癌和胃癌的病因和预防中的作用。五种不同但互补的方法已用于鉴定食道癌易感性基因。首先，已经进行了一项肿瘤/非肿瘤研究，其中由几百例食管癌和胃癌（Cardia and Body）的生物标本库（肿瘤，非肿瘤，静脉血液，手指粘血和颊细胞和颊细胞）组成，在台湾的病毒癌症患者和抗癌癌症的标志性疾病中开发了台湾癌症和胃癌的标识。已经进行了高密度的全基因组扫描，以鉴定潜在的热点，并进一步测试这些热点和其他肿瘤/非肿瘤样品中其他候选标记。还将检查前形态病变。其次，已从高危（扬昌县）和低风险（北京）地区的数百个健康个体收集了DNA的血液样本，以检查选定基因组标记的多态性的潜在人群差异。第三，已经进行了一项针对食管癌和胃癌病例（Cardia和身体）的大型病例对照研究，并用于评估该项目其他组成部分中鉴定出的候选标记中的多态性，并评估基因 - 环境相互作用。第四，一项家庭研究正在进行中，该研究将允许在患有2个或更多病例患有食管癌或胃癌病例的家庭中与癌症的候选标记联系。最后，正在进行一项内窥镜研究，以从正常到早期浸润性食管癌的疾病的形态谱中获得标本，以表征分子进展。