A Differential Dielectric Affinity Microsensor for Stable and Accurate Glucose Mo

一种稳定、准确测量血糖的差分介电亲和微传感器

• 批准号: 8642995
• 负责人: Qiao Lin
• 金额: $ 110万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-30 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8642995
• 关键词: Abdomen; Acute; Address; Adsorption; Adverse effects; Affinity; Animals; Artificial Pancreas; Behavior; Binding; Biocompatible; Biocompatible Materials; Biological Assay; Blood Glucose; Blood capillaries; Boronic Acids; Calibration; Complications of Diabetes Mellitus; Consumption; Detection; Device Designs; Device or Instrument Development; Devices; Diabetes Mellitus; Electrodes; Electronics; Encapsulated; Enzymes; Equilibrium; Foreign-Body Reaction; Future; Glucose; Goals; Gold; Hydrogels; Hypoglycemia; Implant; In Situ; In Vitro; Inflammatory; Insulin; Intercellular Fluid; Lead; Measurement; Measures; Mechanics; Membrane; Methods; Monitor; Needles; Patients; Pattern; Perforation; Physiological; Polymers; Population; Property; Proteins; Public Health; Reaction; Reaction Time; Resistance; Risk; Signal Transduction; Silicon; Skin; Solutions; Subcutaneous Tissue; Surface; System; Technology; Temperature; Testing; Time; Tissues; base; biomaterial compatibility; blood glucose regulation; capillary; chemical reaction; design; dielectric property; electric impedance; experience; flexibility; glucose monitor; glucose sensor; implantable device; implantation; implanted sensor; improved; in vitro testing; in vivo; innovation; miniaturize; minimally invasive; operation; parylene; public health relevance; receptor; response; sensor; surface coating

Continuous glucose monitoring (CGM) involves repetitive measurement of physiological glucose concentration to enable close monitoring and timely correction of problematic blood sugar patterns of patients with diabetes mellitus, thereby reducing the risk of diabetes-related complications and ultimately allowing closed-loop blood sugar monitoring and insulin administration. Commercially available CGM sensors that use electrochemical methods are currently hindered by limitations such as low accuracy (especially at hypoglycemic glucose concentrations), poor stability, and long lag times. We aim to address these issues by developing a subcutaneously implantable affinity microsensor for continuous monitoring of glucose in interstitial fluid. The microsensor, created using microelectromechanical systems (MEMS) technology, will have a miniaturized, flexible design to realize differential measurement of affinity binding of glucose to a synthetic, biocompatible hydrogel. Affinity binding, in which glucose binds specifically and reversibly to the hydrogel without glucose- consuming chemical reactions commonly found in existing glucose sensors, affords high stability. MEMS- based differential measurement of affinity binding enables rapid, accurate determination of glucose concentration, in particular in the hypoglycemic range, in the face of nonspecific disturbances. These functions are realized in a miniaturized, flexible design, which minimizes the effects of device-tissue interactions. In design, the microsensor resides on a flexible substrate and is integrated with a glucose-binding (sensing) hydrogel and a glucose-insensitive (reference) hydrogel. With an active sensing region hundreds of micrometers in size, the device is implanted (via a small needle) beneath the skin in the abdominal region. During operation, glucose molecules in tissue rapidly enter the microsensor and bind reversibly to the sensing hydrogel, whose dielectric properties change accordingly. Meanwhile, the reference hydrogel's dielectric properties change only with nonspecific disturbances (e.g., temperature). Thus, differential dielectric measurement allows accurate determination of the glucose concentration in the interstitial fluid. The direct goal of this project is to develop the differential affinity microsensor for percutaneously implanted operation over a period of 5-7 days with a high level of stability and accuracy. The specific aims include (1) functional hydrogel synthesis, (2) device design and fabrication, and (3) hydrogel and device characterization in vitro and in vivo. The device will in the future be further developed to allow long-term (months or longer) implanted operation, and be included in an artificial pancreas to enable closed-loop glucose control.

连续血糖监测（CGM）包括重复测量生理葡萄糖浓度

项目成果

A hydrogel-based glucose affinity microsensor.

• DOI: 10.1016/j.snb.2016.03.146
• 发表时间: 2016-12
• 期刊: SENSORS AND ACTUATORS B-CHEMICAL
• 影响因子: 8.4
• 作者: Shang, Junyi;Yan, Jing;Zhang, Zhixing;Huang, Xian;Maturavongsadit, Panita;Song, Bing;Jia, Yuan;Ma, Tieying;Li, Dachao;Xu, Kexin;Wang, Qian;Lin, Qiao
• 通讯作者: Lin, Qiao

A graphene-based affinity nanosensor for detection of low-charge and low-molecular-weight molecules.

• DOI: 10.1039/c5nr08866f
• 发表时间: 2016-03-21
• 期刊: Nanoscale
• 影响因子: 6.7
• 作者: Zhu Y;Hao Y;Adogla EA;Yan J;Li D;Xu K;Wang Q;Hone J;Lin Q
• 通讯作者: Lin Q