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Role of mechanically activated ion channels in somatosensation

机械激活离子通道在体感中的作用

基本信息

批准号：
8508241
负责人：
Ardem Patapoutian
金额：
$ 55.43万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-12 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8508241
关键词：
Accounting Acute Afferent Neurons Agonist Alleles Arthritis Attention Behavioral Behavioral Assay Biological Assay Blood Vessels Bradykinin Bradykinin Receptor Calcium Cardiovascular system Cations Cell Line Cells Chemicals Chronic Headaches Data Devices Disease Ear Esthesia Filament Glass Hearing Hyperalgesia Image In Vitro Inflammation Mediators Inflammatory Injury Investigation Ion Channel Knockout Mice Lead Link Mammalian Cell Mastication Mechanics Mechanoreceptors Mediator of activation protein Molecular Morphology Mus Names Nerve Nerve Endings Nervous system structure Neurites Neurons Nociception Nociceptors Organ Pain Peripheral Phenotype Physiological Population Preparation Proteins RNA Interference Reader Role Sensory Signal Pathway Signal Transduction Skin Spinal Ganglia Stress Stretching Structure Symptoms System TNF gene TRP channel TRPV1 gene Temperature Testing Thermal Hyperalgesias Tissues Touch sensation Vertebrates Walking Work follow-up in vivo inflammatory pain joint mobilization knock-down nestin protein neuronal excitability novel preference pressure receptor response sensor small molecule libraries somatosensory sound

项目摘要

DESCRIPTION (provided by applicant): The identity of ion channels involved in sensing mechanical force has remained elusive. Mechanically-activated (MA) cation channels function as touch/pain sensors, and are also required for hearing, adjustment of vascular tone and other functions. Indeed, mechanotransduction is the least understood sensory transduction system at the molecular level. We recently identified MA cation channel components in vertebrates named Piezo1 and Piezo2. Expressing Piezos in a variety of mammalian cell lines induce large MA cationic currents. Furthermore, we show that RNAi against Piezo2 in somatosensory neurons specifically downregulates rapidly-adapting MA cation currents. Here, we test the hypothesis that Piezo2 is required for somatosensory mechanotransduction

描述（由申请人提供）：参与感测机械力的离子通道的身份仍然难以捉摸。机械激活（MA）阳离子通道作为触觉/疼痛传感器，也是听力、血管张力调节和其他功能所需的。事实上，机械转导是分子水平上最不了解的感觉转导系统。我们最近确定了MA阳离子通道组件在脊椎动物命名为Piezo1和Piezo2。在多种哺乳动物细胞系中表达Piezos诱导大的MA阳离子电流。此外，我们表明，RNAi对Piezo2在体感神经元特异性下调快速适应MA阳离子电流。在这里，我们测试的假设，Piezo2是所需的体感机械转导