Angiotensin Metabolite Formation by Podocytes: Implications in Glomerular Injury

足细胞形成血管紧张素代谢物：对肾小球损伤的影响

• 批准号: 8628472
• 负责人: Juan Carlos Q. Velez
• 金额: $ 0.11万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8628472
• 关键词: Affect; Agonist; American; Angiotensin II; Angiotensins; Award; Basic Science; Buffers; Cells; Cellular biology; Chronic Kidney Failure; Clinical; Collagen Type IV; Complex; Data; Deposition; Diabetic Nephropathy; Disease; Endothelial Cells; Enhancers; Ensure; Environment; Enzymes; Equilibrium; Extracellular Matrix Proteins; Extramural Activities; FDA approved; Factor V; Fibronectins; Focal Segmental Glomerulosclerosis; Foundations; Functional disorder; Funding; Future; Gene Targeting; Generations; Goals; Health; Hormones; Image Analysis; Immune Complex Glomerulonephritis; Immunoglobulin A; In Vitro; Individual; Injury; Journals; Kidney; Kidney Diseases; Kidney Glomerulus; Knockout Mice; Knowledge; Laboratories; Laboratory Research; Lead; Link; Longevity; Mass Spectrum Analysis; Mediating; Mediator of activation protein; Medical; Mentors; Mentorship; Metabolism; Nephrology; Neprilysin; Nuclear Magnetic Resonance; Organ; Pathway interactions; Patients; Pattern; Peer Review; Peptides; Peptidyl-Dipeptidase A; Plasminogen Activator Inhibitor 1; Play; Process; Publications; Qualifying; Records; Regulation; Renal Circulation; Renin-Angiotensin System; Research; Research Personnel; Research Proposals; Resource Sharing; Resources; Risk; Role; Scientist; Series; Source; South Carolina; Staging; System; Testing; Therapeutic; Time; Training; Transforming Growth Factors; Ultrafiltration; Universities; career; cell injury; cell type; connective tissue growth factor; design; enzyme activity; experience; glutamyl aminopeptidase; hormone regulation; improved; in vivo; insight; meetings; mesangial cell; multidisciplinary; podocyte; professor; programs; receptor; research study

DESCRIPTION (provided by applicant): This is a new K08 application, the purpose of which is to provide training and a mentored research environment for the candidate, Dr. Juan Carlos Q. Velez, at the Medical University of South Carolina. MUSC is part of South Carolina's largest medical complex with 140,000 ft2 dedicated to research laboratory space and a multidisciplinary University Research Resource Facility (URRF) that contains shared resources available to investigators including: Advanced Image Analysis; Gene targeting and Knockout Mouse Facility; Nuclear Magnetic Resonance Facility, and an established Mass Spectrometry Facility. Candidate. The candidate is a new full-time tenure-track assistant professor in the Division of Nephrology, at the MUSC. He is an up-and-coming young clinician-scientist that has a track record of two awards in basic science research during his early career and one recent first-author publication in a prestigious peer-reviewed journal. He possesses professional qualities highly suited for KO8 sponsorship. Dr. Velez has outlined a plan to develop a successful independently funded laboratory and to gain experience necessary to maintain a productive scientific career relevant to kidney research. To ensure these goals are met, Dr. Velez has assembled a highly qualified mentorship committee of scientists who have extensive track records in obtaining extramural funding and maintaining successful research laboratories. Research Proposal. The proposal has been designed to include a focused hypothesis-driven series of experiments that will yield insight into mechanisms of hormonal regulation within the kidney glomerulus and their relationship with pathways that lead to progressive kidney diseases. Specifically, the proposal is focused on examining the balance between intraglomerular angiotensin-II and other angiotensin metabolites and the role that glomerular podocytes play in that system. The goal of the research proposal is to establish a foundation of technical knowledge and expertise by which a long-term research program can be established.

描述（由申请人提供）： 这是一个新的K 08应用程序，其目的是为候选人Juan卡洛斯Q博士提供培训和指导研究环境。Velez说，他在南卡罗来纳州医科大学工作。MUSC是南卡罗来纳州最大的医疗综合体的一部分，拥有140，000平方英尺的研究实验室空间和一个多学科的大学研究资源设施（URRF），其中包含可供研究人员使用的共享资源，包括：高级图像分析;基因靶向和敲除小鼠设施;核磁共振设施和一个已建立的质谱设施。 候选人这位候选人是MUSC肾脏科的一位新的全职终身助理教授。他是一位崭露头角的年轻临床科学家，在他的早期职业生涯中获得了两项基础科学研究奖项，最近在一份着名的同行评审期刊上发表了一篇第一作者论文。他拥有非常适合KO 8赞助的专业素质。Velez博士概述了一项计划，以建立一个成功的独立资助的实验室，并获得必要的经验，以保持与肾脏研究相关的富有成效的科学事业。为了确保实现这些目标，Velez博士组建了一个高素质的指导委员会，由在获得校外资金和维持成功的研究实验室方面有着广泛记录的科学家组成。 研究提案。该提案旨在包括一系列重点假设驱动的实验，这些实验将深入了解肾小球内激素调节的机制及其与导致进行性肾脏疾病的途径的关系。具体而言，该提案的重点是检查肾小球内血管紧张素II和其他血管紧张素代谢产物之间的平衡，以及肾小球足细胞在该系统中发挥的作用。研究计划的目标是建立一个技术知识和专业知识的基础，通过它可以建立一个长期的研究计划。

项目成果

Lack of renoprotective effect of chronic intravenous angiotensin-(1-7) or angiotensin-(2-10) in a rat model of focal segmental glomerulosclerosis.

• DOI: 10.1371/journal.pone.0110083
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Velez JC;Janech MG;Hicks MP;Morinelli TA;Rodgers J;Self SE;Arthur JM;Fitzgibbon WR
• 通讯作者: Fitzgibbon WR

Enzymatic processing of angiotensin peptides by human glomerular endothelial cells.

人肾小球内皮细胞对血管紧张素肽的酶促加工。

• DOI: 10.1152/ajprenal.00087.2012
• 发表时间: 2012
• 期刊: American journal of physiology. Renal physiology
• 作者: Velez,JuanCarlosQ;Ierardi,JessalynL;Bland,AlisonM;Morinelli,ThomasA;Arthur,JohnM;Raymond,JohnR;Janech,MichaelG
• 通讯作者: Janech,MichaelG

Prolyl carboxypeptidase: a forgotten kidney angiotensinase. Focus on "Identification of prolyl carboxypeptidase as an alternative enzyme for processing of renal angiotensin II using mass spectrometry".

脯氨酰羧肽酶：一种被遗忘的肾血管紧张素酶。

• DOI: 10.1152/ajpcell.00081.2013
• 发表时间: 2013
• 期刊: American journal of physiology. Cell physiology
• 作者: Velez,JuanCarlosQ

Angiotensin I is largely converted to angiotensin (1-7) and angiotensin (2-10) by isolated rat glomeruli.

• DOI: 10.1161/hypertensionaha.109.128819
• 发表时间: 2009-05
• 期刊: Hypertension (Dallas, Tex. : 1979)
• 作者: Velez JC;Ryan KJ;Harbeson CE;Bland AM;Budisavljevic MN;Arthur JM;Fitzgibbon WR;Raymond JR;Janech MG
• 通讯作者: Janech MG