Core D: Analytic Core

烟草烟雾是一种非常复杂的样品，含有4800多种单独的物质(Hoffmann，Hoffmann等人。2001年，Baker，Bishop 2004，Thielena，Klusb等人。2008年)。这些物质的挥发性在很大范围内变化，浓度也是如此。其中一些物质是烟草的添加剂，但大多数是烟草天然成分如纤维素的热解/燃烧产物。相对较高的温度(~950°C)允许发生复杂的反应，产生非常多样化的化合物集合。然而，几乎三分之二的添加剂会经历&lt；5%的分解或反应(Baker，Bishop 2004)。分布在气相之间的化合物是颗粒相(液体气溶胶)。对于有机化合物，约75%在颗粒相，25%在气相。对颗粒进行采样是一项非常具有挑战性的任务。通常将颗粒收集在过滤器上， 提取(有时衍生)，然后用GC/MS或LC/MS分析(图1)。已经知道在这个过程中可以产生人工制品已经有一段时间了。我们已经使用图1所示的设置确认了这一点。我们实验室的研究最近专注于开发新的方法来减少或消除烟雾分析中的收集/提取步骤，以最大限度地减少人工制品的形成。

Tobacco smoke is a very complex sample, containing upwards of 4800 individual substances (Hoffmann, Hoffmann et al. 2001, Baker, Bishop 2004, Thielena, Klusb et al. 2008). The volatility of these substances vanes over a large range as does the concentration. Some of the substances are additives to the tobacco, but most are products of pyrolysis/combustion of natural components of tobacco, e.g. cellulose. The relatively high temperature (~ 950 °C) allows complex reactions to occur, generating a very diverse collection of compounds. However, almost two-thirds of additives undergo <5% decomposition or reaction (Baker, Bishop 2004). The compounds are distributed between the gas phase are particle phase (liquid aerosol). For organic compounds, about 75% are in the particle phase and 25% in the gas phase. Sampling the particles is a very challenging task. Typically particles are collected on filters, extracted (and sometimes derivatized), and then analyzed by GC/MS or LC/MS (Figure 1). It has been known for some time that artifacts can be produced in this procedure. We have confirmed this using the setup shown in Figure 1. Research in our lab has recently been focused on developing new approaches to reduce or eliminate collection/extraction steps in the analysis of smoke, to minimize artifact formation.