DEVELOPMENT OF INSTRUMENTATION FOR BIOMOLECULE ANALYSIS

这项工作的长期目标是开发低成本的分析 仪器和相关分析方法学的改进 生物大分子的结构测定。基质辅助激光器 解吸电离(MALDI)将与四极离子相结合 陷阱。MALDI技术似乎具有许多特征 这将使其优于标准方法电离方法 今天使用的生物分子，快原子轰击(FAB)。MALDI 有可能比FAB具有更好的敏感度， 不太容易发生基质/样品不兼容，并显著产生 质谱图中的化学噪音较少。四极离子陷阱有 以其敏感度和MS/MS能力而闻名，因此 它与MALDI的结合应该会提供巨大的潜力。在……里面 除了优化MALDI与离子陷阱的接口外，Work 以更好地了解细菌的物理化学方面 从生物分子中提取离子的MS/MS。

The long term goal of this work is to develop lower cost analytical instrumentation and the associated analytical methodology for improved structure determination of biomolecules. Matrix-assisted laser desorption ionization (MALDI) will be combined with a quadrupole ion trap. The MALDI technique appears to have a number of characteristics that will make it superior to the standard method ionization method for biological molecules in use today, Fast Atom Bombardment (FAB). MALDI has the potential to have significantly better sensitivity than FAB, is less prone to matrix/sample incompatibilities, and produces significantly less chemical noise in the mass spectrum. The quadrupole ion trap has become well known for its sensitivity and MS/MS capabilities and thus the combination of it with MALDI should offer tremendous potential. In addition to optimizing the interface of MALDI with the ion trap, work will be performed to better understand the physico-chemical aspects of MS/MS of ions from biological molecules.