An Instrument for Negative Acoustic Contrast Bead-based Cell Sorting and Staining

基于负声学对比珠的细胞分选和染色仪器

• 批准号: 8757862
• 负责人: Gabriel P. Lopez
• 金额: $ 23.77万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-20 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8757862
• 关键词: Acoustics; Address; Antibodies; Binding; Biological Assay; Biomedical Research; Blood; Cell Line; Cell Separation; Cell Therapy; Cell model; Cells; Chemosensitivity Assay; Clinical Research; Collection; Communicable Diseases; Complex; Complex Mixtures; Computers; Cytolysis; Diagnosis; Diagnostic; Epithelial Cells; Exhibits; Flow Cytometry; Fluorescent Dyes; Food Safety; Fractionation; Generations; Genes; Genotype; Hematopoietic Stem Cell Transplantation; Human Cell Line; Hybrids; Infectious Disease Epidemiology; Injection of therapeutic agent; Label; Laboratories; Leukocytes; Magnetism; Malignant Neoplasms; Medical; Methodology; Methods; Microfluidics; Modeling; Neoplasm Circulating Cells; Outcome; PTPRC gene; Pharmaceutical Preparations; Phenotype; Population; Preparation; Process; Protocols documentation; Public Health; Public Health Applications Research; Pump; Reagent; Recovery; Regenerative Medicine; Research; Sampling; Series; Silicon; Sorting - Cell Movement; Staining method; Stains; Stem cells; System; TACSTD2 gene; Temperature; Therapeutic; Tissues; Transducers; Tumor Debulking; Ultrasonics; Ultrasonography; Whole Blood; Work; base; cell type; clinically relevant; design; innovation; instrument; instrumentation; next generation; particle; pressure; prototype; public health relevance; regenerative; tool; tumor

DESCRIPTION (provided by applicant): The proposed instrumentation for rapid sorting and staining of cellular samples will provide a convenient tool in a number of cellular analyses and manipulations including diagnoses (e.g., for isolation of circulating tumor cells or circulating feal cells) and therapies (e.g., hematopoietic stem cells transplantation, stem cell based regenerative medicine) based on rare cell isolation from blood or other tissues. While this proposal focuses on simple cell sorting and staining protocols and analysis of cells by multicolor flow cytometry, this versatile, modular system will allow the efficient collection and efficient preparation of cellular samples for a wide variety of clinically relevant analyses such as genotyping/phenotyping assays (e.g., tumor genotyping), drug or chemosensitivity assays, drug reactivity profiles, mutational analyses and high-throughput gene sequencing. The proposed instrumentation will thus have potential impact across a wide cross section of medical applications and general relevance to the improvement of public health.

描述（由申请人提供）：拟议的用于细胞样品快速分选和染色的仪器将为许多细胞分析和操作提供方便的工具，包括诊断（例如，用于分离循环肿瘤细胞或循环粪便细胞）和基于从血液或其他组织中分离的稀有细胞的治疗（例如，造血干细胞移植，基于干细胞的再生医学）。虽然该提案侧重于简单的细胞分选和染色方案以及通过多色流式细胞术对细胞进行分析，但这种多功能模块化系统将允许高效收集和高效制备细胞样品，用于各种临床相关分析，如基因分型/表型分析（例如，肿瘤基因分型），药物或化学敏感性分析，药物反应性谱，突变分析和高通量基因测序。因此，拟议的仪器将在广泛的医疗应用领域产生潜在影响，并与改善公众健康具有普遍相关性。