Animal Models, Histology and Tissue Bank Core

动物模型、组织学和组织库核心

基本信息

批准号：
8741888
负责人：
Robyn S Klein
金额：
$ 13.7万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-25 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8741888
关键词：
Active Immunization Acute Adoptive Transfer Animal Model Animals Autoantigens Biological Markers Biology Blood - brain barrier anatomy CNS autoimmunity Cataloging Catalogs Cell Line Cells Cerebrospinal Fluid Chronic Clinical Demyelinations Dimethyl Sulfoxide Disease Evaluation Experimental Autoimmune Encephalomyelitis Freezing Future Generations Goals Harvest Histology Human Image Immune Immune Cell Activation Infiltration Inflammation Inflammatory Injury Investigation Life Measurement Microscopic Modeling Mouse Strains Multiple Sclerosis Mus Myelin Neuraxis Neuroimmunomodulation Outcome Pathogenesis Patients Peripheral Blood Mononuclear Cell Process Relapse Research Research Personnel Sampling Serum Specimen System T-Lymphocyte Tissue Banking Tissue Banks Tissues Transgenic Organisms Translating Translations Work bench to bedside human tissue in vitro Assay in vivo novel diagnostics programs relating to nervous system remyelination screening

项目摘要

The Animal Models, Histology and Tissue Bank Core will maintain availability and assistance in the induction of all murine models of experimental autoimmune encephalomyelitis (EAE) including monophasic and relapsing-remitting models induced by both active immunization and adoptive transfer of encephalitogenic T cells. Towards this latter goal, Core B will generate and maintain encephalitogenic and control T cell lines. Core B will also assist in all outcome measurements, histological analyses and immunohistochemical approaches. To support PPG investigators¿ exploration of alternative approaches and establish future goals for all Projects, Core B will also establish a murine tissue bank for screening purposes. Because the overall goal of all animals work is to translate findings to human tissues and, ultimately, patients, Core B will establish and maintain a human CNS tissue bank with histopathologic analyses from control and MS patients plus banked peripheral blood mononuclear cells, sera and cerebrospinal fluid. These samples will be utilized by all Projects to validate findings in murine models and to further investigate findings in murine systems using human tissues. We recently hired a dedicated technician to process, catalogue and coordinate the evaluation of CNS specimens for inflammation, demyelination, axonal injury and remyelination. In summary, the function of Core B is to provide technical support for a variety of in vivo experimentation using murine MS models and to facilitate studies for the translation of findings to MS patients via use of human specimens.

动物模型，组织学和组织库核心将保持可用性和帮助实验性自身免疫性脑脊髓炎（EAE）的所有鼠模型的诱导包括由主动免疫和脑源T细胞的过继转移。为了以后的目标，核心B将产生和维持脑生成和控制T细胞系。核心B也将有助于所有结果测量，组织学分析和免疫组织化学方法。支持PPG 调查人员探索替代方法并为所有项目建立未来目标，核心B还将建立一个用于筛查目的的鼠组织库。因为总体所有动物工作的目标是将发现转化为人体组织，最终是患者，核心 B将通过组织病理学分析来建立和维护人类中枢神经系统组织库对照和MS患者加上银行的外周血单核细胞，血清和脑脊液。所有项目都将利用这些样本来验证鼠的发现模型并进一步研究使用人体组织中鼠系统中的发现。我们最近雇用了专门的技术来处理，编目和协调CNS的评估用于感染，脱髓鞘，轴突损伤和透明的标本。总而言之，核心B的功能是为多种体内实验提供技术支持鼠MS模型，并促进研究将发现转化给MS患者的研究人类标本。