Animal Models, Histology and Tissue Bank Core

动物模型、组织学和组织库核心

• 批准号: 8741888
• 负责人: Robyn S Klein
• 金额: $ 13.7万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-25 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8741888
• 关键词: Active Immunization; Acute; Adoptive Transfer; Animal Model; Animals; Autoantigens; Biological Markers; Biology; Blood - brain barrier anatomy; CNS autoimmunity; Cataloging; Catalogs; Cell Line; Cells; Cerebrospinal Fluid; Chronic; Clinical; Demyelinations; Dimethyl Sulfoxide; Disease; Evaluation; Experimental Autoimmune Encephalomyelitis; Freezing; Future; Generations; Goals; Harvest; Histology; Human; Image; Immune; Immune Cell Activation; Infiltration; Inflammation; Inflammatory; Injury; Investigation; Life; Measurement; Microscopic; Modeling; Mouse Strains; Multiple Sclerosis; Mus; Myelin; Neuraxis; Neuroimmunomodulation; Outcome; Pathogenesis; Patients; Peripheral Blood Mononuclear Cell; Process; Relapse; Research; Research Personnel; Sampling; Serum; Specimen; System; T-Lymphocyte; Tissue Banking; Tissue Banks; Tissues; Transgenic Organisms; Translating; Translations; Work; bench to bedside; human tissue; in vitro Assay; in vivo; novel diagnostics; programs; relating to nervous system; remyelination; screening

The Animal Models, Histology and Tissue Bank Core will maintain availability and assistance in the induction of all murine models of experimental autoimmune encephalomyelitis (EAE) including monophasic and relapsing-remitting models induced by both active immunization and adoptive transfer of encephalitogenic T cells. Towards this latter goal, Core B will generate and maintain encephalitogenic and control T cell lines. Core B will also assist in all outcome measurements, histological analyses and immunohistochemical approaches. To support PPG investigators¿ exploration of alternative approaches and establish future goals for all Projects, Core B will also establish a murine tissue bank for screening purposes. Because the overall goal of all animals work is to translate findings to human tissues and, ultimately, patients, Core B will establish and maintain a human CNS tissue bank with histopathologic analyses from control and MS patients plus banked peripheral blood mononuclear cells, sera and cerebrospinal fluid. These samples will be utilized by all Projects to validate findings in murine models and to further investigate findings in murine systems using human tissues. We recently hired a dedicated technician to process, catalogue and coordinate the evaluation of CNS specimens for inflammation, demyelination, axonal injury and remyelination. In summary, the function of Core B is to provide technical support for a variety of in vivo experimentation using murine MS models and to facilitate studies for the translation of findings to MS patients via use of human specimens.

动物模型、组织学和组织库核心将保持可用性和援助 实验性自身免疫性脑脊髓炎小鼠模型的建立 包括主动免疫和主动免疫诱导的单相和复发-缓解模型 脑源性T细胞过继转移。为了实现后一个目标，核心B将产生和 维持脑源性T细胞系和对照T细胞系。核心B也将协助所有结果 测量、组织学分析和免疫组织化学方法。支持PPG 调查人员探索替代方法，并为所有项目确定未来目标， 核心B还将建立一个小鼠组织库，用于筛查目的。因为总体来说 所有动物工作的目标是将发现转化为人体组织，并最终转化为患者，核心 B将建立和维护一个人类中枢神经系统组织库，其组织病理学分析来自 对照组和多发性硬化症患者外周血单核细胞，血清和 脑脊液。这些样本将被所有项目用来验证在小鼠身上的发现 并进一步研究利用人类组织在小鼠系统中的发现。我们最近 聘请专门的技术人员处理、编目和协调CNS的评估 标本用于炎症、脱髓鞘、轴突损伤和再髓鞘形成。总而言之， 核心B的功能是为各种体内实验提供技术支持，使用 小鼠多发性硬化症模型，并促进研究通过使用 人类标本的样本。