Effect of Age on Antibody Diversity

对于小鼠的抗体反应，与雄性相比，对34只雌性小鼠进行灭活流感疫苗接种，可以产生更多的类别转换，身体超突变抗体和更强大的生发中心B细胞反应，从而导致更多样化的H1N1抗原识别和更好的保护，以抵御突变型甲型流感病毒。在开发通用流感疫苗时，应考虑利用方法改善GC B细胞反应和体细胞超突变的频率，特别是在男性中。对于阿尔茨海默病，我们计划对AD小鼠大脑中的B细胞进行系统分析。使用单细胞RNAseq和免疫球蛋白库分析，B细胞将被检查以确定与认知能力下降有关的数量和功能的变化。

For antibody responses in mice, compared with males, inactivated influenza vaccination of 34 female mice causes greater production of class-switched, somatically-hypermutated antibodies and a more robust germinal center B cell response, leading to more diverse H1N1 antigen recognition and better protection against mutant influenza A viruses. Harnessing methods to improve GC B cell responses and frequencies of somatic hypermutations, especially in males, should be considered in the development of universal influenza vaccines. For Alzheimer's disease, we plan to perform a systematic analysis of B cells in the brains of AD mice. Using single-cell RNAseq and immunoglobulin repertoire analysis, B cells will be examined to identify changes in quantity and function in relation to cognitive decline.