Effect of Age on Antibody Diversity

在小鼠的抗体反应方面，与雄性小鼠相比，34只雌性小鼠接种灭活流感疫苗后，会产生更多类别转换、体细胞高度突变的抗体，以及更强大的生发中心B细胞反应，从而导致更多样化的H1N1抗原识别和更好的预防甲型流感变异病毒。在开发通用流感疫苗时，应该考虑利用各种方法来改善GC-B细胞反应和体细胞超突变的频率，特别是在男性中。对于阿尔茨海默病，我们计划对阿尔茨海默病小鼠大脑中的B细胞进行系统分析。使用单细胞RNAseq和免疫球蛋白谱分析，将检查B细胞，以确定与认知能力下降有关的数量和功能的变化。

For antibody responses in mice, compared with males, inactivated influenza vaccination of 34 female mice causes greater production of class-switched, somatically-hypermutated antibodies and a more robust germinal center B cell response, leading to more diverse H1N1 antigen recognition and better protection against mutant influenza A viruses. Harnessing methods to improve GC B cell responses and frequencies of somatic hypermutations, especially in males, should be considered in the development of universal influenza vaccines. For Alzheimer's disease, we plan to perform a systematic analysis of B cells in the brains of AD mice. Using single-cell RNAseq and immunoglobulin repertoire analysis, B cells will be examined to identify changes in quantity and function in relation to cognitive decline.