Acquisition of upgrades for 800 MHz NMR console

获得 800 MHz NMR 控制台的升级

• 批准号: 8639753
• 负责人: Jose Rizorey
• 金额: $ 37.57万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-23 至 2015-09-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639753
• 关键词: Biological; Biomolecular Nuclear Magnetic Resonance; Chemicals; Chromosome Structures; Collaborations; Commit; Communities; Data; Elements; Ensure; Environment; Equipment; Funding; Methodology; Mission; Performance; Postdoctoral Fellow; Publications; Research; Research Personnel; Research Training; Resources; Solubility; Solutions; Source; Structure; System; Training; Work; base; biophysical techniques; cryogenics; graduate student; improved; instrument; instrumentation; interest; programs; protein complex; research study; technology development; tool; trafficking

DESCRIPTION (provided by applicant): We request funds to upgrade the console of an existing Varian Innova 800 MHz solution NMR spectrometer to an Agilent DD2 console, extending the capabilities and lifetime of an essential instrument for biophysical research at UT Southwestern. Several key components that are in good working order, including a passively-shielded 18.8 T superconducting magnet and a 1H{13C,15N} coldprobe with cryogenic system, will be retained. The upgrade will take advantage of technology developments that improve the sensitivity and performance of a broad range of macromolecular and chemical NMR experiments. Further, these replacements will improve the long-term durability of this instrumentation, with benefits to its utility and accessibility. Each of the four investigators sering as PI/co-PIs have established internationally-recognized research programs that use solution NMR to study systems of the utmost biomedical interest. Examples include studies of intracellular trafficking, environmental sensing, cytoskeletal structure and chromosome dynamics; all of these are externally-funded by federal, state and private sources. Collaborations by these researchers and the facility manager enable NMR-based research in over 16 other groups, further expanding the impact of this instrumentation. As the highest-field NMR instrument in this portion of the south-central U.S., this equipment has also provided essential data to researcher's off-campus. The equipment requested in this application will enable researchers to have access to the highest sensitivity, most reliable instrumentation that is essential for challenging studies of proteins and complexes that often have limited solubility o abundance, which traditionally complicate NMR analyses on other instruments. We are committed to providing a world-class environment for biophysical research and training at UT Southwestern. From a research standpoint, the PI and co-PIs view solution NMR as one of the most versatile approaches available for studies of biomolecular structure, function and dynamics. All four of groups pursue challenging applications and develop new methodology, leading to publications which advance our specific biological fields and provide new tools to the NMR community. This research also represents a critical element of the educational mission at UT Southwestern, allowing the training of graduate students and postdocs in this powerful biophysical approach. The impact is extended more broadly to other on- and off-campus groups, by collaboration or providing instrument access at affordable rates. All of these activitie are made possible by the UT Southwestern Biomolecular NMR Facility, leading us to propose upgrading one of its central components to ensure the long-term viability of this resource.

描述（由申请人提供）：我们申请资金将现有Varian Innova 800 MHz溶液NMR光谱仪的控制台升级为Agilent DD 2控制台，延长UT Southwestern生物物理研究所需仪器的功能和寿命。几个工作状态良好的关键部件，包括一个被动屏蔽的18.8 T超导磁体和一个带有低温系统的1H{13 C，15 N}冷探头，将被保留。此次升级将利用技术发展，提高大分子和化学NMR实验的灵敏度和性能。此外，这些更换将提高该仪器的长期耐用性，有利于其实用性和可及性。担任PI/co-PI的四名研究人员中的每一位都建立了国际公认的研究计划，这些计划使用溶液NMR来研究具有最大生物医学意义的系统。例子包括细胞内运输，环境传感，细胞骨架结构和染色体动力学的研究;所有这些都是外部资助的联邦，州和私人来源。这些研究人员和设施经理的合作使其他16个小组能够进行基于NMR的研究，进一步扩大了这种仪器的影响。作为美国中南部这一地区最高场的核磁共振仪器，该设备也为校外的研究人员提供了必要的数据。本申请中要求的设备将使研究人员能够获得最高灵敏度，最可靠的仪器，这对于具有挑战性的蛋白质和复合物研究至关重要，这些蛋白质和复合物通常具有有限的溶解度或丰度，传统上使其他仪器上的NMR分析复杂化。我们致力于为UT西南大学的生物物理研究和培训提供世界一流的环境。从研究的角度来看，PI和co-PI将溶液NMR视为研究生物分子结构，功能和动力学的最通用方法之一。所有四个小组都追求具有挑战性的应用，并开发新的方法，从而推动我们特定的生物领域的出版物，并为NMR社区提供新的工具。这项研究也代表了UT西南大学教育使命的一个关键因素，允许研究生和博士后在这种强大的生物物理方法的培训。通过协作或以负担得起的价格提供仪器访问，其影响更广泛地扩展到其他校内和校外团体。所有这些活动都是由UT西南生物分子NMR设施实现的，这使得我们建议升级其核心组件之一，以确保这种资源的长期可行性。

项目成果

Structural insights for selective disruption of Beclin 1 binding to Bcl-2.

• DOI: 10.1038/s42003-023-05467-w
• 发表时间: 2023-10-24
• 期刊: COMMUNICATIONS BIOLOGY
• 影响因子: 5.9
• 作者: Pan, Yun-Zu;Liang, Qiren;Tomchick, Diana R.;De Brabander, Jef K.;Rizo, Josep
• 通讯作者: Rizo, Josep