Acquisition of upgrades for 800 MHz NMR console

获得 800 MHz NMR 控制台的升级

• 批准号: 8639753
• 负责人: Jose Rizorey
• 金额: $ 37.57万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-23 至 2015-09-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639753
• 关键词: Biological; Biomolecular Nuclear Magnetic Resonance; Chemicals; Chromosome Structures; Collaborations; Commit; Communities; Data; Elements; Ensure; Environment; Equipment; Funding; Methodology; Mission; Performance; Postdoctoral Fellow; Publications; Research; Research Personnel; Research Training; Resources; Solubility; Solutions; Source; Structure; System; Training; Work; base; biophysical techniques; cryogenics; graduate student; improved; instrument; instrumentation; interest; programs; protein complex; research study; technology development; tool; trafficking

DESCRIPTION (provided by applicant): We request funds to upgrade the console of an existing Varian Innova 800 MHz solution NMR spectrometer to an Agilent DD2 console, extending the capabilities and lifetime of an essential instrument for biophysical research at UT Southwestern. Several key components that are in good working order, including a passively-shielded 18.8 T superconducting magnet and a 1H{13C,15N} coldprobe with cryogenic system, will be retained. The upgrade will take advantage of technology developments that improve the sensitivity and performance of a broad range of macromolecular and chemical NMR experiments. Further, these replacements will improve the long-term durability of this instrumentation, with benefits to its utility and accessibility. Each of the four investigators sering as PI/co-PIs have established internationally-recognized research programs that use solution NMR to study systems of the utmost biomedical interest. Examples include studies of intracellular trafficking, environmental sensing, cytoskeletal structure and chromosome dynamics; all of these are externally-funded by federal, state and private sources. Collaborations by these researchers and the facility manager enable NMR-based research in over 16 other groups, further expanding the impact of this instrumentation. As the highest-field NMR instrument in this portion of the south-central U.S., this equipment has also provided essential data to researcher's off-campus. The equipment requested in this application will enable researchers to have access to the highest sensitivity, most reliable instrumentation that is essential for challenging studies of proteins and complexes that often have limited solubility o abundance, which traditionally complicate NMR analyses on other instruments. We are committed to providing a world-class environment for biophysical research and training at UT Southwestern. From a research standpoint, the PI and co-PIs view solution NMR as one of the most versatile approaches available for studies of biomolecular structure, function and dynamics. All four of groups pursue challenging applications and develop new methodology, leading to publications which advance our specific biological fields and provide new tools to the NMR community. This research also represents a critical element of the educational mission at UT Southwestern, allowing the training of graduate students and postdocs in this powerful biophysical approach. The impact is extended more broadly to other on- and off-campus groups, by collaboration or providing instrument access at affordable rates. All of these activitie are made possible by the UT Southwestern Biomolecular NMR Facility, leading us to propose upgrading one of its central components to ensure the long-term viability of this resource.

描述（由申请人提供）：我们要求资金升级现有的Varian Innova 800 MHz解决方案NMR光谱仪的控制台到Agilent DD2控制台，从而扩展了UT西南部生物物理研究基本工具的能力和寿命。将保留几个处于良好工作状态的关键组件，包括被动屏蔽的18.8 T超导磁铁和1H {13C，15n}带有低温系统的冷螺旋头。升级将利用技术发展，以提高大量大分子和化学NMR实验的灵敏度和性能。此外，这些替换将提高该仪器的长期耐用性，并获得其效用和可访问性的好处。四个研究人员中的每一个都作为PI/CO-PIS建立了国际认可的研究计划，这些研究计划使用解决方案NMR来研究最大的生物医学兴趣系统。例子包括对细胞内运输，环境感应，细胞骨架结构和染色体动力学的研究；所有这些都是由联邦，州和私人来源的外部资助。这些研究人员和设施经理的合作使基于NMR的16多个小组的研究进一步扩大了该仪器的影响。作为美国中南部这一部分的最高现场NMR仪器，该设备还为研究人员的校园提供了重要数据。本应用程序中要求的设备将使研究人员能够获得最高敏感性，最可靠的仪器，这对于挑战蛋白质和复合物的挑战至关重要，蛋白质和复合物通常具有有限的溶解度，这传统上使NMR分析复杂化。我们致力于在UT西南部提供世界一流的生物物理研究和培训环境。从研究的角度来看，PI和Co-Pis视图解决方案NMR是用于研究生物分子结构，功能和动力学研究最通用的方法之一。所有四个小组都涉及具有挑战性的应用并开发新的方法，从而导致出版物推进了我们的特定生物学领域，并为NMR社区提供了新的工具。这项研究还代表了UT西南部的教育任务的关键要素，从而允许通过这种强大的生物物理方法对研究生和博士后进行培训。通过协作或以负担得起的价格提供工具访问权限，将其更广泛地扩展到其他校园和校外组。 UT西南生物分子NMR设施使所有这些活动都成为可能，这使我们提出升级其中心组件之一，以确保该资源的长期可行性。

项目成果

Structural insights for selective disruption of Beclin 1 binding to Bcl-2.

• DOI: 10.1038/s42003-023-05467-w
• 发表时间: 2023-10-24
• 期刊: COMMUNICATIONS BIOLOGY
• 影响因子: 5.9
• 作者: Pan, Yun-Zu;Liang, Qiren;Tomchick, Diana R.;De Brabander, Jef K.;Rizo, Josep
• 通讯作者: Rizo, Josep