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Clinical Research Laboratory

临床研究实验室

基本信息

批准号：
8495685
负责人：
Anon Srikiatkhachorn
金额：
$ 69.72万
依托单位：
UNIVERSITY OF RHODE ISLAND
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8495685
关键词：
Acute Address Antigens B-Lymphocytes Biochemistry Biological Assay Biological Process Blood Blood specimen Body Fluids Cell Separation Clinical Clinical Management Clinical Research Complex Culicidae Dengue Dengue Virus Detection Diagnosis Diagnostic Doctor of Philosophy Ensure Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay Epidemiologic Studies Equipment Equipment and supply inventories Flavivirus Flavivirus Infections Flow Cytometry Genes Hemagglutination Hematology Human Resources Immunologics Infection Information Technology Instruction Laboratories Laboratory Research Laboratory Study Logistics Measurement Microbiology Molecular Diagnostic Testing Multiparametric Analysis Pathogenesis Peripheral Blood Mononuclear Cell Philippines Plasma Polymerase Chain Reaction Prevention Procedures Protocols documentation Quality Control Reader Research Research Activity Research Personnel Safety Sampling Secure Serodiagnoses Serological Serotyping Services Shipping Ships Site Southeastern Asia Specimen Specimen Handling Study Subject T-Lymphocyte Temperature Testing Thailand Time Training Vaccines Viral Viral Load result Virus Diseases cold temperature instrument laboratory facility neutralizing antibody operation programs rapid diagnosis viral RNA virology

项目摘要

Clinical Laboratory Core will provide centralized, quality controlled services for specimen processing and management, clinical and virologic laboratory testing, and study-specific tests. The specific functions of the Core are: I. To process biological samples collected in the clinical studies (Project 1, 2) and from dengue vaccine study subjects (Project 3). Core Laboratory will maintain and manage the sample inventory and sample distribution among collaborating laboratories. II. To perform diagnostic serologic and virologic assays for Projects 1, 2 and 3 a. serological diagnosis of acute dengue virus infections using enzyme immunoassay (EIA) and hemagglutination inhibition assay (HAI). b. measurement of dengue serotype-specific neutralizing antibodies by plaque reduction neutralization assay. c. rapid diagnosis of acute dengue virus infections by qualitative dengue virus RT PCR assay. d. isolation of dengue viruses from plasma samples using Toxorrhynchites splendens mosquitoes. III. To quantify the viral burden. a. measurement of dengue virus RNA levels using a fluorogenic RT PCR assay in plasma/serum samples from acutely infected subjects for Projects 1, 2, and 3. b. measurement of NSI antigen levels by serotype-specific ELISA in plasma/serum of acutely ill subjects for Project 1, 2, and 3. IV. To perform flow cytometry analyses on fresh blood samples for Projects 1 e.g. detection of activated T cells and functional characterization of dengue-specific T and B cells during an acute infection.

临床实验室中心将提供集中的，质量控制的服务，标本处理和