14.1 T magnet with +/-1280 G Field Regulation and Integrated MAS Cryogenic System

14.1 T 磁铁，带 /-1280 G 磁场调节和集成 MAS 低温系统

• 批准号: 8734553
• 负责人: TIMOTHY A CROSS
• 金额: $ 137.66万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2017-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8734553
• 关键词: 2,4-Dinitrophenol; Amyloid Fibrils; Capsid; Devices; Electrons; Frequencies; Funding; Funding Applicant; Generations; Investigation; Laboratories; Magic; Microbial Biofilms; Preparation; Regulation; Research Project Grants; Sampling; System; Techniques; United States National Institutes of Health; Viral Matrix Proteins; base; cold temperature; cryogenics; instrument; magnetic field; programs; research study; solid state nuclear magnetic resonance; stem; structural biology; tool

PROJECT SUMMARY Funds are requested for the purchase of a 14.1 T magnet with an 89 mm bore and integrated field regulation to sweep the main field ¿1280 G and to purchase an integrated magic angle spinning (MAS) cryogenic system. The magnet and cryogenics will be vital parts of a 600 MHz DNP NMR system for DNP-enhanced solid state NMR spectroscopic studies of biomolecular systems and materials. This instrument will be part of the first large scale DNP NMR user facility within the U.S., and will be operated as part of the highly successful user program of the National High Magnetic Field Laboratory. This instrument will also serve as the focal point for developing RF probes to enhance the characterization of sensitivity-limited biomolecular systems such as membrane proteins, viral capsid assemblies, amyloid fibrils, and biofilms relevant to NIH funding. In addition, optimized techniques for sample preparation of these delicate samples will be refined. The need for a sweepable widebore 14.1 T magnet, as opposed to the conventional fixed-field magnet used in conventional ssNMR spectrometers, stems from a special need of the gyrotron-based DNP NMR experiment: being narrow-band devices, the sole way that the current generation of gyrotrons can accommodate the multitude of radicals that are demanded for DNP-enhanced biomolecular investigations is to have a capability to change the Larmor frequency of the electrons involved in the experiment. The cryogenic system is required to enable the lowest temperatures possible for both magic angle spinning and oriented biomolecular samples, as the DNP enhancement scales linearly with lower temperatures. The NMR user facilities of the NHMFL already support a broad range of NIH-funded research projects and in this proposal we describe sixteen NIH-funded projects that are at the forefront of developing and utilizing new ssNMR tools for structural biology and will directly benefit from the proposed instrument.

项目概要 需要资金购买 14.1 T 磁铁，孔径为 89 毫米，并配有集成磁场调节装置 横扫主战场 ¿1280 G 并购买集成魔角旋转 (MAS) 低温系统。 磁铁和低温技术将成为 DNP 增强固态 600 MHz DNP NMR 系统的重要组成部分 生物分子系统和材料的核磁共振波谱研究。该仪器将成为第一个大型仪器的一部分 美国境内规模化的 DNP NMR 用户设施，并将作为非常成功的用户计划的一部分进行运营 国家强磁场实验室.该文书还将作为发展的焦点 射频探针可增强灵敏度有限的生物分子系统（例如膜）的表征 与 NIH 资助相关的蛋白质、病毒衣壳组件、淀粉样原纤维和生物膜。此外，还优化了 这些精致样品的样品制备技术将得到改进。需要可扫地 宽口径 14.1 T 磁体，与传统 ssNMR 中使用的传统固定磁场磁体不同 光谱仪，源于基于回旋管的 DNP NMR 实验的特殊需求：窄带 设备，这是当前一代回旋管能够容纳大量自由基的唯一方法 DNP 增强的生物分子研究的要求是有能力改变拉莫尔 参与实验的电子的频率。需要低温系统来实现最低的 魔角旋转和定向生物分子样品均可使用的温度，如 DNP 增强程度随温度降低呈线性变化。 NHMFL 的 NMR 用户设施已经支持 NIH 资助的研究项目范围广泛，在本提案中，我们描述了 16 个 NIH 资助的项目， 处于开发和利用结构生物学新型 ssNMR 工具的前沿，并将直接受益 来自拟议文书。

项目成果

Gadolinium based endohedral metallofullerene Gd2@C79N as a relaxation boosting agent for dissolution DNP at high fields.

• DOI: 10.1039/c7cc09765d
• 发表时间: 2018-03
• 期刊: Chemical communications
• 影响因子: 4.9
• 作者: Xiaoling Wang;Johannes E McKay;Bimala Lama;J. van Tol;Tinghui Li;Kyle Kirkpatrick;Zhehong Gan;Stephen Hill;Joanna R Long;Harry C. Dorn

Mechanistic Insights into the Structural Stability of Collagen-Containing Biomaterials Such as Bones and Cartilage.

• DOI: 10.1021/acs.jpcb.1c01431
• 发表时间: 2021-05-13
• 期刊: The journal of physical chemistry. B
• 作者: Tiwari N;Wi S;Mentink-Vigier F;Sinha N
• 通讯作者: Sinha N

Computationally Assisted Design of Polarizing Agents for Dynamic Nuclear Polarization Enhanced NMR: The AsymPol Family.

• DOI: 10.1021/jacs.8b04911
• 发表时间: 2018-09-05
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Mentink-Vigier F;Marin-Montesinos I;Jagtap AP;Halbritter T;van Tol J;Hediger S;Lee D;Sigurdsson ST;De Paëpe G
• 通讯作者: De Paëpe G

Atomic resolution of cotton cellulose structure enabled by dynamic nuclear polarization solid-state NMR

• DOI: 10.1007/s10570-018-2095-6
• 发表时间: 2019-01-01
• 期刊: CELLULOSE
• 影响因子: 5.7
• 作者: Kirui, Alex;Ling, Zhe;Wang, Tuo
• 通讯作者: Wang, Tuo

Molecular Rationale for Improved Dynamic Nuclear Polarization of Biomembranes.

• DOI: 10.1021/acs.jpcb.6b02885
• 发表时间: 2016-08-18
• 期刊: The journal of physical chemistry. B
• 作者: Smith AN;Twahir UT;Dubroca T;Fanucci GE;Long JR
• 通讯作者: Long JR