14.1 T magnet with +/-1280 G Field Regulation and Integrated MAS Cryogenic System

项目总结 请拨资金用于购买14.1T直径为89毫米的磁体和综合现场调节，以 扫描主场？1280G，并购买集成魔角旋转(MAS)低温系统。 磁体和低温装置将是600 MHz DNP固体增强核磁共振系统的重要组成部分 生物分子系统和材料的核磁共振波谱研究。这台仪器将成为第一个大型 Scale DNP核磁共振用户设施在美国境内，并将作为非常成功的用户计划的一部分进行操作 国家强磁场实验室的。这一工具也将作为发展的焦点 用于增强膜等灵敏度受限生物分子系统表征的RF探头 与NIH资助相关的蛋白质、病毒衣壳组件、淀粉样纤维和生物膜。此外，还优化了 这些精致样品的样品制备技术将得到改进。对可清扫汽车的需求 宽带14.1T磁体，而不是传统的SS核磁共振中使用的常规固定磁场磁体 光谱仪是回旋管DNP核磁共振实验的一种特殊需求：窄带 设备，当代回旋管可以容纳大量的自由基的唯一方式 DNP增强的生物分子研究所需的是具有改变Larmor的能力 参与实验的电子的频率。需要低温系统才能使最低 魔角旋转和定向生物分子样品的可能温度，如DNP 随着温度的降低，增强效果呈线性增长。NHMFL的核磁共振用户设施已经支持 NIH资助的广泛的研究项目，在这份提案中，我们描述了NIH资助的16个项目 处于开发和利用用于结构生物学的新的单核磁共振工具的前沿，并将直接受益 从拟议的文书中删除。

PROJECT SUMMARY Funds are requested for the purchase of a 14.1 T magnet with an 89 mm bore and integrated field regulation to sweep the main field ¿1280 G and to purchase an integrated magic angle spinning (MAS) cryogenic system. The magnet and cryogenics will be vital parts of a 600 MHz DNP NMR system for DNP-enhanced solid state NMR spectroscopic studies of biomolecular systems and materials. This instrument will be part of the first large scale DNP NMR user facility within the U.S., and will be operated as part of the highly successful user program of the National High Magnetic Field Laboratory. This instrument will also serve as the focal point for developing RF probes to enhance the characterization of sensitivity-limited biomolecular systems such as membrane proteins, viral capsid assemblies, amyloid fibrils, and biofilms relevant to NIH funding. In addition, optimized techniques for sample preparation of these delicate samples will be refined. The need for a sweepable widebore 14.1 T magnet, as opposed to the conventional fixed-field magnet used in conventional ssNMR spectrometers, stems from a special need of the gyrotron-based DNP NMR experiment: being narrow-band devices, the sole way that the current generation of gyrotrons can accommodate the multitude of radicals that are demanded for DNP-enhanced biomolecular investigations is to have a capability to change the Larmor frequency of the electrons involved in the experiment. The cryogenic system is required to enable the lowest temperatures possible for both magic angle spinning and oriented biomolecular samples, as the DNP enhancement scales linearly with lower temperatures. The NMR user facilities of the NHMFL already support a broad range of NIH-funded research projects and in this proposal we describe sixteen NIH-funded projects that are at the forefront of developing and utilizing new ssNMR tools for structural biology and will directly benefit from the proposed instrument.