TR&D3-SCH

TR

• 批准号: 10217179
• 负责人: TIMOTHY A CROSS
• 金额: $ 12.67万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217179
• 关键词: Biological; Biological Process; Biomedical Research; Cell Nucleus; Cellular Membrane; Chemistry; Chills; Complex; Coupled; Crystallization; Crystallography; Detection; Development; Dimensions; Feedback; Future; Goals; High temperature of physical object; Hybrids; Lead; Lipid Bilayers; Measurement; Membrane Proteins; Metals; Molecular Structure; Molecular Weight; NMR Spectroscopy; Noise; Oxygen; Power Sources; Proteins; Protons; Regulation; Relaxation; Residual state; Resolution; Resources; Sampling; Scientist; Series; Signal Transduction; Site; Spectrum Analysis; Speed; Structure; Technology; Temperature; Testing; Time; Work; biological systems; cold temperature; experimental study; frontier; improved; macromolecule; magnetic field; new technology; next generation; restraint; solid state nuclear magnetic resonance; structural biology; tool; water flow

TR&D3 - Project Summary/Abstract Significant increases in field strength have always led to new frontiers in the scientific arenas approachable by NMR spectroscopy. Often the challenges and what has been viewed as the drawbacks to high fields have proven to some of their greatest advantages. The Series Connected Hybrid (SCH) magnet, at a field of 36T, that will become operational in the summer of 2016 at the NHMFL will provide a view into the future for NMR spectroscopy; a future in which High Temperature Superconducting (HTS) magnets will have the potential for doubling the field strengths of current Low-Temperature superconducting (LTS) magnets. The SCH magnet is a hybrid magnet having an outer superconducting coil of 13T and inner stacks of Bitter plates that will be powered by 14MW and cooled by deionized water flowing through the stacks at 1700 gal/min. The SCH magnet will be the highest homogeneity and the highest stability resistive magnet that has been constructed, but the homogeneity and stability will not be the same as that for LTS magnets. The BTRR will develop a biomedical research focus on this magnet through the development of additional technology using a cross section of scientists committed to developing and pushing the scientific frontiers of NMR spectroscopy at a field strength that is more than 50% higher than any other NMR spectrometer in the world. More than a decade of effort has now gone into developing new technologies to enhance the stability and homogeneity of this magnet. Two approaches are being taken: first, an advanced lock unit has been developed by Bruker and tested at the NHMFL and secondly, Prof. Jeffrey Schiano from Penn State has developed a cascade field regulation technology that takes advantage of an inductive pickup coil as well as NMR measurements to provide a feedback field correction. A new 1H detection HXY 1.3 mm MAS probe will be developed for structural biology solid state NMR. 1H detection has the potential to revolutionize solid state NMR in much the same way 1H detection revolutionized solution NMR 30 years ago. This revolution will open will facilitate the structural characterization of membrane proteins in lipid bilayers and potentially in native cellular membrane. This technology will be coupled with oriented samples ssNMR that provides complementary structural restraints. The signal to noise per unit of spectrometer time for spin ½ nuclei improves with approximately B03, but for quadrupolar nuclei the rate of enhancement can be even greater due to a wide variety of factors including relaxation effects. While there are many odd halves quadrupolar nuclei in biological systems the most common and important one is 17O. Oxygen atoms are the primary sites in biological macromolecules of catalytic and functional chemistry. A goal in this effort is to work toward developing 17O spectroscopy as a routine spectroscopic tool.

Tr&d3-项目摘要/摘要 场强的显著增强总是导致科学领域的新领域。 可通过核磁共振光谱分析来接近。通常情况下，挑战和被视为缺点的 高地已经证明了它们的一些最大优势。串联混合动力(SCH)磁体，位于 一个36T的油田将于2016年夏天在NHMFL投入使用，从这里可以看到 核磁共振光谱学的未来；高温超导(HTS)磁体的未来 将当前低温超导(LTS)磁体的场强提高一倍的潜力。这个 SCH磁体是一种混合磁体，具有13T的外部超导线圈和内部堆叠的BITY磁片 它将由14兆瓦的电力提供动力，并以1700加仑/分钟的速度通过电堆的去离子水进行冷却。这个 SCH磁体将是最高的均匀性和最高稳定性的阻性磁体 但其均匀性和稳定性将不同于LTS磁体。BTRR将 通过开发附加技术，重点研究这种磁铁的生物医学研究 致力于开发和推动核磁共振光谱学科学前沿的科学家群体，请访问 一个比世界上任何其他核磁共振光谱仪都高出50%以上的场强。 经过十多年的努力，现已开发出新技术，以增强稳定性和 这块磁铁的均匀性。目前正在采取两种方法：第一，开发了一种先进的锁具装置 首先，来自宾夕法尼亚州立大学的杰弗里·斯基亚诺教授开发了一种 利用感应拾取线圈和核磁共振的级联调场技术 测量以提供反馈场校正。一个新的1H检测HXY 1.3 mm MAS探头将是 为结构生物学开发的固态核磁共振。1H检测有可能给固态技术带来革命性的变化 核磁共振的方式与30年前1H检测使溶液核磁共振发生革命性变化的方式大致相同。这场革命将开启 将有助于膜蛋白在脂质双层中的结构表征，并可能在天然的 细胞膜。这项技术将与定向样品ss核磁共振相结合，提供 结构性约束相辅相成。 对于自旋1/2的原子核，每单位光谱仪时间的信噪比提高了大约B03，但是 对于四极核，由于多种因素的影响，增强的速度可能会更大，包括 松弛效应。虽然在生物系统中有许多奇数一半的四极核，但最常见的 重要的一点是17O。氧原子是生物大分子中催化和催化的主要位置 功能化学。这项工作的一个目标是致力于将17O光谱分析作为例行公事 光谱工具。